Gamma irradiated soluble extracts of Toxoplasma gondii tachyzoites induced better humoral and cellular immune response due to preferential uptake by APCs scavenger receptors
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2017
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American Journal of Tropical Medicine and Hygiene
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Resumo
Toxoplasmosis occurs in one-third of the adult world population, without
adequate vaccines and causing disease in fetus or specific groups. Aside
to sterilizing effect, gamma radiation acts on antigens inducing enhanced
antisera production against snake venoms or cell and humoral response
to recombinant leprosy proteins. Gamma radiation affects proteins directly
or indirectly in water by action of oxidant radicals from water radiolysis.
Early reports showed gamma irradiated crotoxin had enhanced uptake by
macrophages, limited by scavenger receptors competitors, as probucol.
Irradiated tachyzoites induced adequate immune response with protection,
attributed to mitotic death and DNA damage. Irradiated proteins could
take a part in this process and we study the immune response induced
by gamma irradiated soluble extracts of Toxoplasma gondii tachyzoites,
using mice immunized with native proteins as controls. Mice immunized
with irradiated extracts without adjuvants showed significant protection
after challenge with ME-49 (p<0.05) and RH (p<0.0001) strains compared
to controls. There are increased specific and high avidity IgG production
(p<0.05) when compare d to controls group. By flow cytometry and in
vitro culture, spleens of mice immunized irradiated extract presented
increased proliferation of CD4+, CD8+ and B cells and IFN-γ production
as compared to controls. J774 cells had increased uptake of biotinylated
irradiated extracts as compared to the uptake of native extract (p<0.05),
due to longer and continuous uptake. All these data points to an
alternative and effective uptake and immune processing of irradiated T.
gondii extracts, probably due to specific receptor of oxidized proteins
as scavenger receptors, resulting in enhanced immunity. This data also
implies that irradiated proteins could be involved in the protection induced
by irradiated parasites. Use of antigen gamma radiation can be a simple
process to enhance vaccine efficiency, avoiding the use of adjuvants.
Como referenciar
COSTA, ANDREA da; ZORGI, NAHIARA E.; NASCIMENTO, NANCI do; GALISTEO JUNIOR, ANDRES J.; ANDRADE JUNIOR, HEITOR F. de. Gamma irradiated soluble extracts of Toxoplasma gondii tachyzoites induced better humoral and cellular immune response due to preferential uptake by APCs scavenger receptors. American Journal of Tropical Medicine and Hygiene, v. 95, n. 5, p. 174-174, 2017. S. DOI: 10.4269/ajtmh.abstract2016. Disponível em: http://repositorio.ipen.br/handle/123456789/31159. Acesso em: 14 Mar 2025.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.