Genotoxic evaluation of [DOTA,Tyrsup(3)]octreotate labeled with sup(131)I and sup(177)Lu in human peripheral lymphocytes in vitro by micronucleus assay

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dc.contributor.authorSUZUKI, M.F.pt_BR
dc.contributor.authorSILVA, M.A.pt_BR
dc.contributor.authorCALDEIRA FILHO, J.S.C.pt_BR
dc.contributor.authorCOLTURATO, M.T.pt_BR
dc.contributor.authorARAUJO, E.B.pt_BR
dc.contributor.authorBARTOLINI, P.pt_BR
dc.contributor.authorOKAZAKI, K.pt_BR
dc.coverageInternacionalpt_BR
dc.creator.eventoINTERNATIONAL NUCLEAR ATLANTIC CONFERENCE; ENCONTRO NACIONAL DE APLICACOES NUCLEARES, 7thpt_BR
dc.date.accessioned2014-11-17T18:44:30Zpt_BR
dc.date.accessioned2014-11-18T18:49:43Zpt_BR
dc.date.accessioned2015-04-01T20:39:46Z
dc.date.available2014-11-17T18:44:30Zpt_BR
dc.date.available2014-11-18T18:49:43Zpt_BR
dc.date.available2015-04-01T20:39:46Z
dc.date.eventoago. 28 - set. 2, 2005pt_BR
dc.description.abstractThe radiolabeled receptor-binding peptides have being used for cancer diagnosis and therapy. The octreotate, a somatostatin analogue peptide, bound to various tumors expressing sst receptors (thyroid, pancreas, prostrate, melanoma and lymphomas). The amount and the type of receptors for somatostatin influence the tissue uptake. The [DOTA, Tyr3 ]octreotate has been used because of its high affinity to somatostatin subtype receptors sstr2 and sstr5. The pharmacokinetic study showed that the blood clearance is rapid and only 9% of the intravenous injected activity remains in human blood after one hour. The aim of this study was to evaluate the cytogenetic effect of radiolabeled [DOTA, Tyr3 ]octreotate in blood cells in vitro, using the cytokinesis-block micronucleus (MN) assay. This technique allows evaluating the mutagenic effects of both endogenous and exogenous agents at chromosome level. Blood samples of healthy donors were collected in heparinized syringes and exposed to different activities of [DOTA, Tyr3 ]octreotate labeled with with 131I (n=3) and 177Lu (n=3), where radioactive concentration ranged from 600 to 5600 kBq/mL, corresponding to an injected activity of 3.1 to 28.9 GBq in a reference man of 70 kg weight. 131I and 177Lu are beta- and gamma-emitters. After one-hour exposition to radiopharmaceuticals at 37o C, the cells were washed with culture medium for removing the non internalised octreotate and cultivated for 72 hours, according to criteria adopted by the IAEA. The results showed a positive correlation between radioactive concentrations (X) and the frequency of binucleated cells with micronuclei (Y) (P<0.05). The model for the best fit of data was the linear one (Y = a + bX). The equation for [131I-DOTA, Tyr3 ]octreotate was Y = (1.634 ± 0.236) + (0.912 ± 0.137) 10-3 X and for [177Lu-DOTA, Tyr3 ]octreotate was Y = (1.715 ± 0.342) + (0.743 ± 0.135) 10-3 X. The non labeled molecule, [DOTA, Tyr3 ]octreotate, has no influence in the induction of cytogenetic damage. The micronucleus assay with rat pancreatic tumor cells (AR42J) that express the sstr2 receptor for somatostatin, submitted to these radiopharmaceuticals, are in course.
dc.event.siglaINAC,2005; ENAN,7pt_BR
dc.identifier.citationSUZUKI, M.F.; SILVA, M.A.; CALDEIRA FILHO, J.S.C.; COLTURATO, M.T.; ARAUJO, E.B.; BARTOLINI, P.; OKAZAKI, K. Genotoxic evaluation of [DOTA,Tyrsup(3)]octreotate labeled with sup(131)I and sup(177)Lu in human peripheral lymphocytes in vitro by micronucleus assay. In: INTERNATIONAL NUCLEAR ATLANTIC CONFERENCE; ENCONTRO NACIONAL DE APLICACOES NUCLEARES, 7th, ago. 28 - set. 2, 2005, Santos, SP. <b>Anais...</b> Disponível em: http://repositorio.ipen.br/handle/123456789/18254.
dc.identifier.orcidhttps://orcid.org/0000-0003-0456-5589
dc.identifier.urihttp://repositorio.ipen.br/handle/123456789/18254pt_BR
dc.local.eventoSantos, SPpt_BR
dc.publisherSao Paulo: ABEN, 2005pt_BR
dc.rightsopenAccesspt_BR
dc.subjectdose-response relationshipspt_BR
dc.subjectexperimental datapt_BR
dc.subjectgenetic radiation effectspt_BR
dc.subjectin vitropt_BR
dc.subjectiodine 131pt_BR
dc.subjectlabelled compoundspt_BR
dc.subjectlutetium 177pt_BR
dc.subjectlymphocytespt_BR
dc.subjectneoplasmspt_BR
dc.subjectsomatostatinpt_BR
dc.subjecttoxicitypt_BR
dc.titleGenotoxic evaluation of [DOTA,Tyrsup(3)]octreotate labeled with sup(131)I and sup(177)Lu in human peripheral lymphocytes in vitro by micronucleus assaypt_BR
dc.typeTexto completo de eventopt_BR
dspace.entity.typePublication
ipen.autorMARIA TEREZA COLTURATO
ipen.autorKAYO OKAZAKI
ipen.autorPAOLO BARTOLINI
ipen.autorELAINE BORTOLETI DE ARAUJO
ipen.autorJOSE DE SOUZA CALDEIRA FILHO
ipen.autorMARTA ADELINA DA SILVA
ipen.autorMIRIAM FUSSAE SUZUKI
ipen.codigoautor434
ipen.codigoautor1220
ipen.codigoautor1503
ipen.codigoautor664
ipen.codigoautor1155
ipen.codigoautor3281
ipen.codigoautor557
ipen.contributor.ipenauthorMARIA TEREZA COLTURATO
ipen.contributor.ipenauthorKAYO OKAZAKI
ipen.contributor.ipenauthorPAOLO BARTOLINI
ipen.contributor.ipenauthorELAINE BORTOLETI DE ARAUJO
ipen.contributor.ipenauthorJOSE DE SOUZA CALDEIRA FILHO
ipen.contributor.ipenauthorMARTA ADELINA DA SILVA
ipen.contributor.ipenauthorMIRIAM FUSSAE SUZUKI
ipen.date.recebimento05-10pt_BR
ipen.event.datapadronizada2005pt_BR
ipen.identifier.ipendoc10585pt_BR
ipen.notas.internasAnaispt_BR
ipen.type.genreArtigo
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sigepi.autor.atividadeSUZUKI, M.F.:557:18:Spt_BR
sigepi.autor.atividadeSILVA, M.A.:3281:7:Npt_BR
sigepi.autor.atividadeCALDEIRA FILHO, J.S.C.:1155:12:Npt_BR
sigepi.autor.atividadeCOLTURATO, M.T.:434:12:Npt_BR
sigepi.autor.atividadeARAUJO, E.B.:664:12:Npt_BR
sigepi.autor.atividadeBARTOLINI, P.:1503:18:Npt_BR
sigepi.autor.atividadeOKAZAKI, K.:1220:18:Npt_BR
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