Flow cytometry based micronucleus assay for evaluation of genotoxic potential of 2-ACBs in hepatic cells HepG2

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dc.contributor.authorBARBEZAN, ANGELICA B.
dc.contributor.authorSANTOS, CARLA J.B.
dc.contributor.authorCARVALHO, LUMA R.
dc.contributor.authorVIEIRA, DANIEL P.
dc.contributor.authorSANTELLI, GLAUCIA M.M.
dc.contributor.authorVILLAVICENCIO, ANNA L.C.H.
dc.coverageInternacionalpt_BR
dc.creator.eventoINTERNATIONAL NUCLEAR ATLANTIC CONFERENCEpt_BR
dc.date.accessioned2018-01-04T12:10:40Z
dc.date.available2018-01-04T12:10:40Z
dc.date.eventoOctober 22-27, 2017pt_BR
dc.description.abstractFood irradiation is approved for use in more than 60 countries for applications and purposes in a wide variety of foods, being an effective and safe method for preservation and long-term storage. 2-Alkylcyclobutanones (2-ACBs) are the only known radiolytic products generated from foods that contain fatty acids (Triglycerides) when irradiated. The acids analyzed in this study are palmitic and stearic, which when irradiated form 2-Dodecylcyclobutanones (2-dDCB) and 2-Tetradecylcyclobutanone (2-tDCB). Part of the 2-ACBs ingested is excreted through feces and part is deposited in adipose tissues. In vitro studies so far have been only in colon cells. The work used a human hepatoma cell line (HepG2) since the accumulation of fat in this organ is quite common. Micronucleus test was selected to evaluate possible genotoxic effects of 2-dDCB and 2-tDCB compounds when exposed to high concentrations (447, 1422 and 2235 μM) for 4 and 24 hours. Tests were performed in quadriplicates using flow cytometric analysis. None detectable genotoxic damage was observed after 4 hours of exposure to the compounds, and cytotoxic effects were only significant at the highest concentration (2235 μM) of 2-dDCB. After 24 hours of exposure, slight genotoxic damage was observed at all concentrations evaluated, and cytotoxic effects were only present when exposed to compound 2-tDCB. Although there is a genotoxic and cytotoxic effect in some of the situations tested, the two compounds predominantly induced proliferation reduction effects of this hepatic tumor cell line.pt_BR
dc.event.siglaINACpt_BR
dc.identifier.citationBARBEZAN, ANGELICA B.; SANTOS, CARLA J.B.; CARVALHO, LUMA R.; VIEIRA, DANIEL P.; SANTELLI, GLAUCIA M.M.; VILLAVICENCIO, ANNA L.C.H. Flow cytometry based micronucleus assay for evaluation of genotoxic potential of 2-ACBs in hepatic cells HepG2. In: INTERNATIONAL NUCLEAR ATLANTIC CONFERENCE, October 22-27, 2017, Belo Horizonte, MG. <b>Proceedings...</b> Rio de Janeiro, RJ: Associação Brasileira de Energia Nuclear, 2017. Disponível em: http://repositorio.ipen.br/handle/123456789/28220.
dc.identifier.orcidhttps://orcid.org/0000-0001-6199-7877
dc.identifier.orcidhttps://orcid.org/0000-0002-0007-534X
dc.identifier.urihttp://repositorio.ipen.br/handle/123456789/28220
dc.localRio de Janeiro, RJpt_BR
dc.local.eventoBelo Horizonte, MGpt_BR
dc.publisherAssociação Brasileira de Energia Nuclearpt_BR
dc.rightsopenAccesspt_BR
dc.subjectcell flow systems
dc.subjectdodecyl radicals
dc.subjectfood processing
dc.subjecthepatomas
dc.subjecthexadecanoic acid
dc.subjectin vitro
dc.subjectirradiation
dc.subjectoctadecanoic acid
dc.subjectradiation effects
dc.subjecttoxicity
dc.titleFlow cytometry based micronucleus assay for evaluation of genotoxic potential of 2-ACBs in hepatic cells HepG2pt_BR
dc.typeTexto completo de eventopt_BR
dspace.entity.typePublication
ipen.autorCARLA JULIANA BALBINO DOS SANTOS
ipen.autorANNA LUCIA CASANAS HAASIS VILLAVICENCIO
ipen.autorDANIEL PEREZ VIEIRA
ipen.autorLUMA RAMIREZ DE CARVALHO
ipen.autorANGELICA BUENO BARBEZAN
ipen.codigoautor14679
ipen.codigoautor1372
ipen.codigoautor3158
ipen.codigoautor12133
ipen.codigoautor6417
ipen.contributor.ipenauthorCARLA JULIANA BALBINO DOS SANTOS
ipen.contributor.ipenauthorANNA LUCIA CASANAS HAASIS VILLAVICENCIO
ipen.contributor.ipenauthorDANIEL PEREZ VIEIRA
ipen.contributor.ipenauthorLUMA RAMIREZ DE CARVALHO
ipen.contributor.ipenauthorANGELICA BUENO BARBEZAN
ipen.date.recebimento18-01pt_BR
ipen.event.datapadronizada2017pt_BR
ipen.identifier.ipendoc24045pt_BR
ipen.notas.internasProceedingspt_BR
ipen.type.genreArtigo
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relation.isAuthorOfPublicationc2352608-be9c-4a73-be8c-571f10bb53d2
relation.isAuthorOfPublication8e39b9fc-86ca-4b06-bf2a-cb8f163165d8
relation.isAuthorOfPublication27082032-b0c8-462c-8d6c-767873119adf
relation.isAuthorOfPublication.latestForDiscovery27082032-b0c8-462c-8d6c-767873119adf
sigepi.autor.atividadeBARBEZAN, ANGELICA B.:6417:210:Spt_BR
sigepi.autor.atividadeSANTOS, CARLA J.B.:14679:-1:Npt_BR
sigepi.autor.atividadeCARVALHO, LUMA R.:12133:810:Npt_BR
sigepi.autor.atividadeVIEIRA, DANIEL P.:3158:810:Npt_BR
sigepi.autor.atividadeVILLAVICENCIO, ANNA L.C.H.:1372:210:Npt_BR
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