Photodynamic inactivation of antibiotic resistant strain of Pseudomonas aeruginosa in vivo

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dc.contributor.authorHASHIMOTO, M.C.E.pt_BR
dc.contributor.authorTOFFOLI, D.J.pt_BR
dc.contributor.authorPRATES, R.A.pt_BR
dc.contributor.authorCOURROL, LILIA C.pt_BR
dc.contributor.authorRIBEIRO, M.S.pt_BR
dc.coverageInternacionalpt_BR
dc.creator.eventoWORLD CONGRESS OF THE INTERNATIONAL PHOTODINAMIC ASSOCIATION CONFERENCE PHOTODYNAMIC THERAPY: BACK TO THE FUTURE, 12thpt_BR
dc.date.accessioned2014-11-17T18:22:24Zpt_BR
dc.date.accessioned2014-11-18T18:31:37Zpt_BR
dc.date.accessioned2015-04-02T00:35:33Z
dc.date.available2014-11-17T18:22:24Zpt_BR
dc.date.available2014-11-18T18:31:37Zpt_BR
dc.date.available2015-04-02T00:35:33Z
dc.date.eventoJune 11-15, 2009pt_BR
dc.description.abstractBurns are frequently contamined by pathogenic microorganisms and the widespread occurrence of antibiotic resistant strains of Pseudomonas aeruginosa in hospitals is a matter of growing concern. Hypocrellin B (HB) is a new generation photosensitizer extracted from the fungus Hypocrella bambusae with absorption bands at 460, 546 and 584 nm. Lanthanide ions change the HB molecular structure and a red shift in the absorption band is observed as well as an increase in the singlet oxygen quantum yield. In this study, we report the use of HB:La+3 to kill resistant strain of P. aeruginosa infected burns. Burns were produced on the back of mice and wounds were infected subcutaneously with 1x109 cfu/mL of P. aeruginosa. Three-hours after inoculation, the animals were divided into 4 groups: control, HB:La+3, blue LED and HB:La+3+blue LED. PDT was performed using 10µM HB:La+3 and 500mW light-emitting diode (LED) emitting at λ=470nm±20nm during 120s. The animals of all groups were killed and the infected skin was removed for bacterial counting. Mice with photosensitizer alone, light alone or untreated infected wounds presented 1x108 cfu/g while mice PDT-treated showed a reduction of 2 logs compared to untreated control. These results suggest that HB:La+3 associated to blue LED is effective in diminishing antibiotic resistant strain P. aeruginosa in infected burns.
dc.identifier.citationHASHIMOTO, M.C.E.; TOFFOLI, D.J.; PRATES, R.A.; COURROL, LILIA C.; RIBEIRO, M.S. Photodynamic inactivation of antibiotic resistant strain of Pseudomonas aeruginosa in vivo. In: WORLD CONGRESS OF THE INTERNATIONAL PHOTODINAMIC ASSOCIATION CONFERENCE PHOTODYNAMIC THERAPY: BACK TO THE FUTURE, 12th, June 11-15, 2009, Seattle, WA. <b>Proceedings...</b> Disponível em: http://repositorio.ipen.br/handle/123456789/16702.
dc.identifier.urihttp://repositorio.ipen.br/handle/123456789/16702pt_BR
dc.local.eventoSeattle, WApt_BR
dc.rightsopenAccesspt_BR
dc.subjectpseudomonaspt_BR
dc.subjecthypocrellin bpt_BR
dc.subjectburnspt_BR
dc.subjectskinpt_BR
dc.subjectphotosensitivity
dc.subjecttherapy
dc.titlePhotodynamic inactivation of antibiotic resistant strain of Pseudomonas aeruginosa in vivopt_BR
dc.typeTexto completo de eventopt_BR
dspace.entity.typePublication
ipen.autorMARCOS SOBRAL RIBEIRO
ipen.autorLILIA CORONATO COURROL
ipen.autorRENATO ARAÚJO PRATES
ipen.autorDANIEL JOSÉ TOFFOLI
ipen.autorMARIA CRISTINA EIKO HASHIMOTO
ipen.codigoautor3273
ipen.codigoautor1750
ipen.codigoautor4028
ipen.codigoautor5945
ipen.codigoautor5881
ipen.contributor.ipenauthorMARCOS SOBRAL RIBEIRO
ipen.contributor.ipenauthorLILIA CORONATO COURROL
ipen.contributor.ipenauthorRENATO ARAÚJO PRATES
ipen.contributor.ipenauthorDANIEL JOSÉ TOFFOLI
ipen.contributor.ipenauthorMARIA CRISTINA EIKO HASHIMOTO
ipen.date.recebimento09-12pt_BR
ipen.event.datapadronizada2009pt_BR
ipen.identifier.ipendoc14341pt_BR
ipen.notas.internasProceedingspt_BR
ipen.type.genreArtigo
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relation.isAuthorOfPublicatione8c0783f-8cac-4bcb-946d-5634e2d893b8
relation.isAuthorOfPublication.latestForDiscoverye8c0783f-8cac-4bcb-946d-5634e2d893b8
sigepi.autor.atividadeHASHIMOTO, M.C.E.:5881:920:Spt_BR
sigepi.autor.atividadeTOFFOLI, D.J.:5945:910:Npt_BR
sigepi.autor.atividadePRATES, R.A.:4028:920:Npt_BR
sigepi.autor.atividadeCOURROL, LILIA C.:1750:920:Npt_BR
sigepi.autor.atividadeRIBEIRO, M.S.:3273:-1:Npt_BR
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