Radionuclidic purity of 68Ga eluted from 68Ge/68Ga generators by Thin Layer Chromatography
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EJNMMI Radiopharmacy and Chemistry
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Background: 68Ga is a PET emitter radionuclide with an important role
in nuclear medicine diagnosis procedures. The physical half-life of 68
min is compatible with the pharmacokinetics of many biomolecules
and low molecular weight substrates. Another important feature is its
availability from a generator system, where the parent radionuclide,
68Ge (t1/2=270.95 days) is adsorbed on a column and daughter, 68Ga, is
eluted in ionic form 68Ga3+. The objective of this work was to develop
a new method for the determination of the radionuclidic purity [1] of
68Ga, allowing a fast determination of the 68Ge breakthrough in 68Ga
eluates.
Materials and methods: The method to evaluate the 68Ge impurity
in 68Ga samples recommended by the European Pharmacopeia is to
measure the activity of 68Ga eluted from the generator, allow 24 h
decay and measure the same sample. All 68Ga activity will come from
the decay of 68Ge, so the level of 68Ge is calculated. The proposed
methodology was based on the different behaviour of Ge and Ga in
thin layer/paper chromatography [2]. Several solvents and strips were
tested, as well as different solutions added to the spot point to interact with the species. The detection and quantification limits were calculated for the best system to evaluate the possibility of reaching the
maximum level of 68Ge given in the monograph.
Results: The best system was achieved using TLC-SG-IB-F strips, acetone as solvent and a solution of 3 mol L−1
HCl added to the spot point
of the strip. The retention factors were 0.1 for 68Ge and 0.5-0.6 for 68Ga,
allowing to cut the strips into 2 pieces for measuring in the detector.
The detection and quantification limits showed that the strip sample
could be measured 2 h after the beginning of the quality control procedure, then correcting to the full growth of 68Ge. Samples were measured using the traditional and the proposed method and the values
were similar.
Conclusions: The proposed method is simple, fast and allow the evaluation of the 68Ge impurity in samples of 68Ga freshly eluted from the
generators.
Como referenciar
TCHOBNIAN, TANIA P.B.; OSSO JUNIOR, JOAO A. Radionuclidic purity of 68Ga eluted from 68Ge/68Ga generators by Thin Layer Chromatography. EJNMMI Radiopharmacy and Chemistry, v. 9, n. 65, p. 21-21, 2024. Suppl 1. DOI: 10.1186/s41181-024-00294-8. Disponível em: https://repositorio.ipen.br/handle/123456789/49134. Acesso em: 20 Jan 2026.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.