Effective methodology for maintaining Toxoplasma gondii in vitro using paramagnetic iron nanoparticles to support three-dimensional cell culture
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2021
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Biophysical Reviews
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INTRODUCTION
Toxoplasma gondii is a protozoan parasite that infects approximately one
billion people worldwide. Upon infection, the host may die due to latent
infection or presence with chronic cysts in brain, retina or muscle tissue.
Humans can become infected consuming water or foods contaminated
with oocysts or eating undercooked meat. Its virulent form is difficult to
replicate in vitro, requiring additional steps using experimental animals.
The use of nanotechnology can contribute to this in vitro production,
through the three-dimensional cultivation of mouse fibroblast cells
(NIH / 3T3 ATCC ® CRL-1658™) and nanoparticles synthesized with
radiation.
OBJECTIVES
The objective of this work was to demonstrate the three-dimensional
culture of fibroblast cells aggregated to nanoparticles for inoculation the
T. gondii.
MATERIALS AND METHODS
This methodology was created to facilitate parasite management and replication.
For the production of nanoparticles, the work used concentrations
of iron sulfate II heptahydrate (Fe2SO4.7H2O, CAS 7782-63-0) and
glycine (NH2CH2COOH, CAS 56-40-6) diluted in ultrapure water free ofO2 at pH 12. This solutionwas irradiated by electron beam of the IPEN
/ CNEN-SP Radiation Technology Center in doses of at least 15 and at
most 30kGy. Paramagnetic iron oxide nanoparticles (PION’s) were then
adsorbed on cell membranes, and cells were kept together by a magnetic
field. Structured spheroids (4 day of culture) were infected with 106
parasites (RH strain) and the infection was evaluated by transmission
electron microscopy.
DISCUSSION AND RESULTS
Tachyzoiteswere found inside 3T3 cells, assuring that the spheroid can be
a suitable culture substrate to T. gondii in vitro propagation.
CONCLUSION
A three-dimensionalmethodology for in vitro cultivation of the parasite is
perhaps the key for applications in the study of toxoplasmosis, as it has a
fast, cheap, efficient production (yield and reduction of contamination).
Como referenciar
NASCIMENTO, ANA C.G.; GALISTEO JUNIOR, ANDRES J.; SILVA, GIOVANA D. da; ROCHA, LEONARDO W.P. de S.; VIEIRA, DANIEL P. Effective methodology for maintaining Toxoplasma gondii in vitro using paramagnetic iron nanoparticles to support three-dimensional cell culture. Biophysical Reviews, v. 13, n. 6, p. 1466-1466, 2021. DOI: 10.1007/s12551-021-00845-2. Disponível em: http://repositorio.ipen.br/handle/123456789/32891. Acesso em: 06 Mar 2025.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.