Blyes and fet: a preliminary understanding between effects and total estrogenic activity on surface waters
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Applied Research in Toxicology
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Resumo
Endocrine disrupting chemicals (EDCs) are included in a
range of substances and are present in practically all
environmental matrices, generally at low concentrations.
For the occurrence evaluation of these substances, several
in vitro tests are employed, among of them Bioluminescent
Yeast Estrogen Screening (BLYES), which measures the
total estrogenic activity in EEQ. However, this assay is not
able to provide information about adverse effects to
aquatic organisms. In order to observe aquatic
communities effects, organic extracts of surface water
samples from rivers and reservoirs of São Paulo state with
results above the detection limit in BLYES (> 0.1 EEQ)
were performed with embryonic assays with Danio rerio.
The methodology was conducted according OECD 236, to
verify acute effects such as: absence of heart beats,
absence of somites, no tail detachment and coagulated
embryo. Embryonic malformations were evaluated, such
as: reduction of organism size, cardiac and vitelline edema,
spine curvature and reduction of heart beating, which are
characteristics of chronic effects. Among the 21 tested
samples, 24% showed acute effects for Danio rerio and
14%, chronic effects. The effects observed probably would
be associated with EDCs and/or other contaminants that
may bind to the receptor in BLYES assay. The information
obtained by embryonic assay with Danio rerio was suitable
to show the effects of these groups of contaminants of the
organic extracts and would complement the BLYES
response. Accomplishing tests to evaluate effects on
embryo reproductive system to establish a correlation
between estrogenic activity and acute and chronic effects
observed on FET is necessary.
Como referenciar
MARTINI, G.A.; VIVEIROS, W.; QUINAGLIA, G.; FERREIRA, M.L.; ROGERO, S.O.; ROGERO, J.R. Blyes and fet: a preliminary understanding between effects and total estrogenic activity on surface waters. Applied Research in Toxicology, v. 2, p. 43-43, 2017. suppl. 1. Disponível em: http://repositorio.ipen.br/handle/123456789/28112. Acesso em: 24 Mar 2026.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.