Photodynamic therapy induces epidermal thickening in hairless mice skin: an optical coherence tomography assessment
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2014
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OPTICAL COHERENCE TOMOGRAPHY AND COHERENCE DOMAIN OPTICAL METHODS IN BIOMEDICINE, 18th
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Photodynamic therapy (PDT) promotes skin improvement according to many practitioners, however the immediately in
vivo assessment of its response remains clinically inaccessible. As a non-invasive modality, optical coherence
tomography (OCT) has been shown a feasible optical diagnostic technique that provides images in real time, avoiding
tissue biopsies. For this reason, our investigation focused on evaluates the PDT effect on a rodent model by means of
OCT. Therefore, a normal hairless mouse skin has undergone a single-session PDT, which was performed with topical 5-
aminolevulinic acid (ALA) cream using a red (630 nm) light emitting diode (LED) which reached the light dose of 75
J/cm2. As the optical imaging tool, an OCT (930 nm) with axial resolution of 6.0 microns in air was used, generating
images with contact to the mouse skin before, immediately after, 24 hours, and 2 weeks after the correspondent
procedure. Our result demonstrates that, within 24 hours after ALA-PDT, the mouse skin from the PDT group has shown
epidermal thickness (ET), which has substantially increased after 2 weeks from the treatment day. Moreover, the skin
surface has become evener after ALA-PDT. Concluding, this investigation demonstrates that the OCT is a feasible and
reliable technique that allows real-time cross-sectional imaging of skin, which can quantify an outcome and predict
whether the PDT reaches its goal.
Como referenciar
JORGE, ANA E.S.; CAMPOS, CAROLINA P.; FREITAS, ANDERSON Z. de; BAGNATO, VANDERLEI S. Photodynamic therapy induces epidermal thickening in hairless mice skin: an optical coherence tomography assessment. In: IZATT, JOSEPH A. (ed.); FUJIMOTO, JAMES G. (ed.); TUCHIN, VALERY V. (ed.). In: OPTICAL COHERENCE TOMOGRAPHY AND COHERENCE DOMAIN OPTICAL METHODS IN BIOMEDICINE, 18th, February 01, 2014, San Francisco, CA, USA. Proceedings... Bellingham, WA, USA: International Society for Optics and Photonics, 2014. p. 89343N-1 - 89343-3. (SPIE Proceedings Series, 8934). DOI: 10.1117/12.2040443. Disponível em: http://repositorio.ipen.br/handle/123456789/27653. Acesso em: 30 Dec 2025.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.