Human bone morphogenetic protein (hBMP)-2 characterization by physical chemical, immunological and biological assays
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Journal of Biotechnology
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Commercial preparations of human-met-BMP-2 (GenScript)
and of CHO-derived hBMP-2 (Infuse-Medtronic) provided a complete
characterization of this protein, which belongs to the
“transforming growth factors β” superfamily, via SDS-PAGE,
Western blotting, reversed-phase HPLC, high-performance size-exclusion
chromatography and MALDI-TOF-MS.
E.coli-derived met-hBMP-2 has shown a large presence of dimer
(MM= 26,054 Da), versus a theoretic value of 26,072 Da. More complex
was the distribution of the CHO-derived product, whose
exact MM has never been reported due to variable glycosylation:
via MALDI-TOF-MS a dimer (28,732 Da) and a large amount of
monomer (14,377 Da) were found. A novel method based on RP-HPLC
was also validated for hBMP-2 qualitative and quantitative
analysis directly in ongoing culture media. The classical “in vitro”
bioassay, via alkaline phosphatase induction in murine myoblastic
cells C2C12, confirmed that hBMP-2 bioactivity is mostly related to
the dimer, being ∼6-fold higher for the CHO-derived glycosylated
form.
Considering that hBMP-2 is a highly effective osteoinductors,
plays an important role during bone regeneration and repair, as well as during embryonic development, and presents an extremely
high aggregate value, we believe that these data pave the way to
the characterization of this important factor when obtained by DNA
recombinant techniques in different host cells.
Como referenciar
SUZUKI, M.F.; OLIVEIRA, J.E.; DAMIANI, R.; LIMA, E.R.; AMARAL, K.C.; SILVA, F.M.; BARTOLINI, P. Human bone morphogenetic protein (hBMP)-2 characterization by physical chemical, immunological and biological assays. Journal of Biotechnology, v. 305, p. S10-S10, 2019. S. DOI: 10.1016/j.jbiotec.2019.05.050. Disponível em: http://repositorio.ipen.br/handle/123456789/30934. Acesso em: 29 Apr 2026.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.