Incorporation of the methylene blue photosensitizer in the presence of glucose in strains of candida albicans with multidrug efflux systems

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dc.contributor.authorSILVA, TAMIRES
dc.contributor.authorALVARENGA, LETICIA
dc.contributor.authorLEAL, CINTIA
dc.contributor.authorMIRANDA, BIANCA G.
dc.contributor.authorSUZUKI, LUIZ C.
dc.contributor.authorKATO, ILKA
dc.contributor.authorRIBEIRO, MARTHA
dc.contributor.authorPAVANI, CHRISTIANE
dc.contributor.authorPRATES, RENATO
dc.coverageInternacionalpt_BR
dc.date.accessioned2017-10-17T17:11:35Z
dc.date.available2017-10-17T17:11:35Z
dc.date.issued2017pt_BR
dc.description.abstractBackground: Infectious diseases are responsible for 60% of deaths in developing countries.Candida albicans cause severe infection in immunocompromised patients and the commonly used treatments have not been effective against microbial resistance. Photodynamic therapy (PDT) is a new promising strategy to microbial inactivation based on the use of photosensitizer (PS) in the presence of oxygen and activation by light to form reactive oxygen species.C.albicanshas glucose sensors which could facilitate the entrance of the PS. The objective of this study was to evaluate the role of glucose in the photodynamic inactivation of C. albicans. Study: C. albicans ATCC 10231, YEM 12, YEM 13, YEM 14, and YEM15were aerobically grown on Sabouraud agar and incubated at 308C for 24 hours. Microbial inoculants were divided into 4 groups with and without glucose: Control; Only irradiation; PS toxicity and PDT groups with three irradiation times.After going throughthe treatments, the colony-forming units were counted and the data were subjected to statistical analysis (ANOVA) and Tukey test. To measure the concentration ofMB, fluorescence spectroscopy and flow cytometry were used. Results: We observed that yeast with overexpression of Major Facilitator Superfamily membrane pore tend to accumulate more MB in its cytoplasm, whereas strains that overexpress ABC pumps demonstrated the greater survival against the photodynamic challenge. Conclusion: PDT is an efficient strategy against C. albicans and the presence of glucose can intervene in the photodynamic effect. The presence of MB in the intracellular region of the yeast predisposes to greater photodynamic inactivation, however, its germicidal capacity is related to the type and characteristics of the microbial.pt_BR
dc.format.extent438-438pt_BR
dc.identifier.citationSILVA, TAMIRES; ALVARENGA, LETICIA; LEAL, CINTIA; MIRANDA, BIANCA G.; SUZUKI, LUIZ C.; KATO, ILKA; RIBEIRO, MARTHA; PAVANI, CHRISTIANE; PRATES, RENATO. Incorporation of the methylene blue photosensitizer in the presence of glucose in strains of candida albicans with multidrug efflux systems. <b>Lasers in Surgery and Medicine</b>, v. 49, n. 4, p. 438-438, 2017. Disponível em: http://repositorio.ipen.br/handle/123456789/27922.
dc.identifier.fasciculo4pt_BR
dc.identifier.issn0196-8092pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-4203-1134
dc.identifier.urihttp://repositorio.ipen.br/handle/123456789/27922
dc.identifier.vol49pt_BR
dc.relation.ispartofLasers in Surgery and Medicinept_BR
dc.rightsopenAccesspt_BR
dc.sourceAMERICAN SOCIETY FOR LASER MEDICINE AND SURGERY, 37th, Apr 2017, San Diego, CA, USApt_BR
dc.titleIncorporation of the methylene blue photosensitizer in the presence of glucose in strains of candida albicans with multidrug efflux systemspt_BR
dc.typeResumos em periódicospt_BR
dspace.entity.typePublication
ipen.autorRENATO ARAÚJO PRATES
ipen.autorMARTHA SIMOES RIBEIRO
ipen.autorILKA TIEMY KATO
ipen.codigoautor4028
ipen.codigoautor574
ipen.codigoautor3089
ipen.contributor.ipenauthorRENATO ARAÚJO PRATES
ipen.contributor.ipenauthorMARTHA SIMOES RIBEIRO
ipen.contributor.ipenauthorILKA TIEMY KATO
ipen.date.recebimento17-10pt_BR
ipen.identifier.fi2.312pt_BR
ipen.identifier.ipendoc23233pt_BR
ipen.identifier.iwosWoSpt_BR
ipen.range.fi1.500 - 2.999
ipen.type.genreResumo
relation.isAuthorOfPublicationfde20222-8ca2-4a9e-941b-516590cac130
relation.isAuthorOfPublication36215a53-0150-4910-91d7-9559717b62d7
relation.isAuthorOfPublication6c5e2bd6-1ae0-41e8-a0f6-9b29d0c1b115
relation.isAuthorOfPublication.latestForDiscovery6c5e2bd6-1ae0-41e8-a0f6-9b29d0c1b115
sigepi.autor.atividadeKATO, ILKA:3089:920:Npt_BR
sigepi.autor.atividadeRIBEIRO, MARTHA:574:920:Npt_BR
sigepi.autor.atividadePRATES, RENATO:4028:920:Npt_BR
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