Europium tetracycline biosensor for the determination of cholesterol
Carregando...
Data
Data de publicação
2007
Autores IPEN
Orientador
Título da Revista
ISSN da Revista
Título do Volume
É parte de
É parte de
É parte de
É parte de
ADVANCED BIOMEDICAL AND CLINICAL DIAGNOSIS, 5th
Exportar
Resumo
Development of cholesterol biosensors is of great importance in clinical analysis because the concentration of
cholesterol in blood is a fundamental parameter for the prevention and diagnosis of a number of clinical disorders such
as heart disease, hypertension and arteriosclerosis. In general, determination of cholesterol is based on
spectrophotometry; but this method involves complicated procedures and the cost is high because expensive enzyme
must be used in each assay.
We report here the observation, for the first time, of the enhancement of Europium-Tetracycline complex
emission in cholesterol solutions. This enhancement was initially observed with the addition of the enzyme cholesterol
oxidase, which produces H2O2, the agent driver of the Europium tetracycline complex, to the solution. However, it was
found that the enzyme is not needed to enhance the luminescence. A calibration curve was determined, resulting in an
easy-handling immobilization method with a cheap stable material. This method shows that the complex can be used as a
sensor to determine cholesterol in biological systems with good selectivity, fast response, miniature size, and
reproducible results.
Como referenciar
COURROL, LILIA C.; SILVA, FLAVIA R. de O.; SAMAD, RICARDO E.; MANSANO, RONALDO D.; VIEIRA JUNIOR, NILSON D. Europium tetracycline biosensor for the determination of cholesterol. In: ADVANCED BIOMEDICAL AND CLINICAL DIAGNOSIS, 5th, January, 2007, San Jose, USA. Proceedings... Disponível em: http://repositorio.ipen.br/handle/123456789/19311. Acesso em: 26 Mar 2026.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.