Metacyclic promastigotes of Leishmania amazonensis selection using gamma irradiation
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2005
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INTERNATIONAL NUCLEAR ATLANTIC CONFERENCE; ENCONTRO NACIONAL DE APLICACOES NUCLEARES, 7th
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Leishmania spp. causes a spectrum of human diseases, ranging from self-healing skin lesions to severe and
lethal visceral disease. In previous work we demonstrated that the protein and nucleic acid metabolism and
oxidative respiration were severely affected by irradiation, in a dose response way, but a small but
representative fractions are relatively radio resistant, surviving after 800 Gy of 60Co irradiation. The best
explanation could be a selection of metacyclic promastigotes. In these forms, the G0 state allows the adequate
correction of DNA repair after the irradiation insult. In this work, we are looking for the ideal radiation dose to
select the higher proportion of metacyclic forms of L.. (L.) amazonensis in culture. Parasites were grown in
RPMI 1640 medium, plus 20% fetal calf serum, than they were irradiated with differents doses rangin between
25 and 400 Gy. Parasites irradiated at 400 Gy infected, proportionally, more cells than parasites irradiated at
other doses. To confirm this metacyclogenesis, a complement lysis assay was performed with 5, 10 and 20% of
male guinea pig blood serum at 20ºC for 3 hours, and parasites counted. Guinea pig serum a 10% promotes
more lysis, with 200 Gy irradiated parasites being less affected, probably due to metacyclic selection. These
preliminary results suggests that the ionizing radiation, specially between 200 and 400 Gy, could be a alternative
tool for the selection of metacyclic forms of Leishmania amazonensis in culture.
Como referenciar
BONETTI, F.C.; ANDRADE JUNIOR, H.F.; NASCIMENTO, N. Metacyclic promastigotes of Leishmania amazonensis selection using gamma irradiation. In: INTERNATIONAL NUCLEAR ATLANTIC CONFERENCE; ENCONTRO NACIONAL DE APLICACOES NUCLEARES, 7th, ago. 28 - set. 2, 2005, Santos, SP. Anais... Disponível em: http://repositorio.ipen.br/handle/123456789/17655. Acesso em: 30 Dec 2025.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.