Optical and histological evaluation in human tendon tissue sterilized by ionizing radiation
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2017
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INTERNATIONAL NUCLEAR ATLANTIC CONFERENCE
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Sterilization by irradiation is a technique that is used by tissue banks aiming to eliminate contamination of human
allografts, being a safe method, free of residue and used as final sterilization. After the tissue procurement, these
undergo a series of processing stages and then are packaged and preserved by freezing. Despite aseptic care of the
material those may be subjected to sterilization in the final packing by ionizing radiation, raising the security level
of sterility of the tissue. The aim of this study was to evaluate the effects of application of ionizing radiation,
produced by 60Co source in human tendons preprocessed (A-alcohol + antibiotic; B- H2O2 + ultrasound) obtained
through collaboration with tissue banks and preserved by freezing in -80° C, the radiation absorbed doses in
processing were 12.5, 15 and 25 kGy, each one with their corresponding non-irradiated control, to examine possible
structural or morphological alterations. The irradiated samples and their controls were analyzed by means of optical
coherence tomography (OCT) and optical coherence tomography polarization sensitive (PS-OCT), and histological
tests had been stained with hematoxylin-eosin (HE). According to the results the tissue processed with
alcohol/antibiotic in conjunction with irradiation proved to be the most effective.
Como referenciar
FUNARI, ANA P.; ANTEBI, URI; SANTOS, LUIZ A.; VIEIRA, DANIEL P.; MIRANDA, JURANDIR T. de; ALVES, NELSON M.; FREITAS, ANDERSON Z. de; MATHOR, MONICA B. Optical and histological evaluation in human tendon tissue sterilized by ionizing radiation. In: INTERNATIONAL NUCLEAR ATLANTIC CONFERENCE, October 22-27, 2017, Belo Horizonte, MG. Proceedings... Rio de Janeiro, RJ: Associação Brasileira de Energia Nuclear, 2017. Disponível em: http://repositorio.ipen.br/handle/123456789/28277. Acesso em: 27 Mar 2026.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.