Identification of irradiated wheat by germination test, DNA comet assay and electron spin resonance

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dc.contributor.authorBARROS, A.C.pt_BR
dc.contributor.authorFREUND, M.T.L.pt_BR
dc.contributor.authorVILLAVICENCIO, A.L.C.H.pt_BR
dc.contributor.authorDELINCEE, H.pt_BR
dc.contributor.authorARTHUR, V.pt_BR
dc.contributor.editorSADAT, T.pt_BR
dc.contributor.editorEHLERMANN, D.pt_BR
dc.contributor.editorMILLER, A.pt_BR
dc.coverageInternacionalpt_BR
dc.creator.eventoINTERNATIONAL MEETING ON RADIATION PROCESSING, 12thpt_BR
dc.date.accessioned2014-11-17T18:13:45Zpt_BR
dc.date.accessioned2014-11-18T18:14:30Zpt_BR
dc.date.accessioned2015-04-02T03:21:37Z
dc.date.available2014-11-17T18:13:45Zpt_BR
dc.date.available2014-11-18T18:14:30Zpt_BR
dc.date.available2015-04-02T03:21:37Z
dc.date.eventoMar. 25-30, 2001pt_BR
dc.description.abstractIn several countries, there has been an increase in the use of radiation for food processing thus improving the quality and sanitary conditions, inhibiting pathogenic microorganisms, delaying the natural aging process and so extending product lifetime. The need to develop analytical methods to detect these irradiated products is also increasing. The goal of this research was to identify wheat irradiated using different radiation doses. Seeds were irradiated with a gamma 60Co source (Gammacell 220 GC) in the Centro de Energia Nuclear na Agricultura and the Instituto de Pesquisas Energeticas e Nucleares. Dose rate used were 1.6 and 5.8 kGy/h. Applied doses were 0.0, 0.10, 0.25, 0.50, 0.75, 1.0, and ! 2.0 kGy. After irradiation, seeds were analysed over a 6 month period. Three different detection methods were employed to determine how irradiation had modified the samples. Screening methods consisted of a germination test measuring the inhibition of shooting and rooting and analysis of DNA fragmentation. The method of electron spin resonance spectroscopy allowed a better dosimetric evaluation. These techniques make the identification of irradiated wheat with different doses possible. r 2002 Elsevier Science Ltd. All rights reserved.
dc.event.siglaIMRP,12pt_BR
dc.format.extent423-426pt_BR
dc.identifier.citationBARROS, A.C.; FREUND, M.T.L.; VILLAVICENCIO, A.L.C.H.; DELINCEE, H.; ARTHUR, V. Identification of irradiated wheat by germination test, DNA comet assay and electron spin resonance. In: SADAT, T. (ed.); EHLERMANN, D. (ed.); MILLER, A. (ed.). In: INTERNATIONAL MEETING ON RADIATION PROCESSING, 12th, Mar. 25-30, 2001, Avignon, France. <b>Proceedings...</b> p. 423-426. Disponível em: http://repositorio.ipen.br/handle/123456789/16364.
dc.identifier.orcidhttps://orcid.org/0000-0001-6199-7877
dc.identifier.urihttp://repositorio.ipen.br/handle/123456789/16364pt_BR
dc.local.eventoAvignon, Francept_BR
dc.rightsopenAccesspt_BR
dc.sourcePublicado tambem em: Radiat. Phys. Chem., , v. 63, n. 3/6, p. 423-426, 2002pt_BR
dc.subjectfood processingpt_BR
dc.subjectirradiationpt_BR
dc.subjectwheatpt_BR
dc.subjectgamma radiationpt_BR
dc.subjectcobalt 60pt_BR
dc.subjectgerminationpt_BR
dc.subjectdnapt_BR
dc.subjectelectrophoresispt_BR
dc.subjectstrand breakspt_BR
dc.subjectelectron spin resonancept_BR
dc.titleIdentification of irradiated wheat by germination test, DNA comet assay and electron spin resonancept_BR
dc.typeTexto completo de eventopt_BR
dspace.entity.typePublication
ipen.autorANNA LUCIA CASANAS HAASIS VILLAVICENCIO
ipen.codigoautor1372
ipen.contributor.ipenauthorANNA LUCIA CASANAS HAASIS VILLAVICENCIO
ipen.date.recebimento03-09pt_BR
ipen.event.datapadronizada2001pt_BR
ipen.identifier.ipendoc09115pt_BR
ipen.notas.internasProceedingspt_BR
ipen.type.genreArtigo
relation.isAuthorOfPublication8ce58b2f-23cf-45b5-a8c4-0fb067965096
relation.isAuthorOfPublication.latestForDiscovery8ce58b2f-23cf-45b5-a8c4-0fb067965096
sigepi.autor.atividadeBARROS, A.C.:3793:-1:Spt_BR
sigepi.autor.atividadeFREUND, M.T.L.:-1:-1:Npt_BR
sigepi.autor.atividadeVILLAVICENCIO, A.L.C.H.:1372:-1:Npt_BR
sigepi.autor.atividadeDELINCEE, H.:3738:-1:Npt_BR
sigepi.autor.atividadeARTHUR, V.:2635:-1:Npt_BR
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