Evaluation of moniotoxic activity of bothropstoxin-1 irradiated with sup(60)Co gamma rays
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2009
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INTERNATIONAL NUCLEAR ATLANTIC CONFERENCE; MEETING ON NUCLEAR APPLICATIONS, 9th; MEETING ON REACTOR PHYSICS AND THERMAL HYDRAULICS, 16th; MEETING ON NUCLEAR INDUSTRY, 1st
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Ionizing radiation has been successfully employed to modify the immunological properties of biomolecules and
has been proven to be a powerful tool to attenuate snake venoms toxicity without affecting their immunogenic
properties. Snake venoms and their isolated toxins showed structural modifications after gamma radiation
process, in aqueous solution, mainly by water radiolysis sub products. Free radical scavengers, such as NaNO3
and t-butanol, present selective effects on specific radical from water radiolysis. The NaNO3 has affinity by
aqueous electron, while the t-butanol has affinity by hydroxyl radical. At the present work, we have investigated
the miotoxic activity of bothropstoxin-1 (BTHX-1), a K49 phospholipase, present in Bothrops jararacussu
crude venom, before and after irradiation process, with or without scavenger substances presence. BTHX-1 was
irradiated with 2kGy of 60Co gamma rays, in aqueous solution and in the presence of oxygen. BALB/c mice
were inoculated with either native or irradiated toxin, with or without scavenger substances. After 3 hours, blood
samples were collected and the miotoxic activity was evaluated by LDH (lactate dehydrogenase) release. The
muscular tissue damage was directly related to the LDH amounts released. Irradiated bothropstoxin-1, with or
without NaNO3 substance, caused less damage than their native counterpart. But irradiated toxin, in the presence
of t-butanol, was so miotoxic as the native BTHX-1. These results indicate that irradiation of toxic proteins can
promote significant modifications on their structures, but still retaining many of the original biological
properties of their native counterparts. Additionally, some scavengers substances can change these gamma
radiation effects.
Como referenciar
MIRCO, JESSICA; BAPTISTA, JANAINA A.; CAPRONI, PRISCILA; YOSHITO, DANIELE; NASCIMENTO, NANCI. Evaluation of moniotoxic activity of bothropstoxin-1 irradiated with sup(60)Co gamma rays. In: INTERNATIONAL NUCLEAR ATLANTIC CONFERENCE; MEETING ON NUCLEAR APPLICATIONS, 9th; MEETING ON REACTOR PHYSICS AND THERMAL HYDRAULICS, 16th; MEETING ON NUCLEAR INDUSTRY, 1st, September 27 - October 2, 2009, Rio de Janeiro, RJ. Proceedings... Disponível em: http://repositorio.ipen.br/handle/123456789/16578. Acesso em: 24 Mar 2026.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.