Biochemical characterization of skin burn wound healing using ATR-FTIR
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2018
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SBFOTON INTERNATIONAL OPTICS AND PHOTONICS CONFERENCE
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Resumo
Efficient biochemical characterization of skin
burn healing stages can improve clinical routine to adjust
patients treatment. The golden standard for diagnosing skin
burning stages is the histological biopsy. This practice is
often expensive and technically challenging. There have been
advances in the treatment, and diagnostic of the critical skin
burned patients due to the increase of multidisciplinary
collaboration. The contributions from different fields of
biomedical engineering motivate to develop a better
procedure for clinical applications. Considering the difficulty
of monitoring wound healing the Fourier Transform
Infrared coupled with an Attenuated Total Reflectance
(ATR-FTIR) accessory is an analytical technique that can
provide information regarding spectral biomarkers in
biological materials. This study aimed to evaluate the
classification feasibility provided by ATR-FTIR technique in
the burned skin to follow the regenerative process in vivo. 40
skin burned samples from the Wistar rats dorsum at 3,7, 14,
21 days after burn were compared with the corresponded
healthy group samples, by registering their infrared
absorption spectra in FTIR Thermo Nicolet 6700 coupled to
a diamond crystal ATR. The spectra were separated in the
region 900 to 1800 cm-1 for further chemometric calculations.
The second derivative of spectra was applied for
discrimination, which results demonstrated differences from
control and burns wounded groups, as well as among, burn
wounded groups, using Amide I (1628 cm-1) and Amide II
(1514 cm-1) bands. Amide I and Amide II bands are two
significant bands of the infrared protein spectrum. The
Amide I band is mainly associated with the C=O stretching
vibration (70-80%) and is directly related to the backbone
conformation. The Amide II band results from the N-H
bending vibration (40-60%) and from the C-N stretching
vibration (18-40%). This band is conformationally sensitive.
These bands suggest proteins activity changing associate to
inflammatory and maturation stages when it is compared
with the healthy group. The statistical difference with amide
I occur in proliferation and maturation stages. These
findings indicate that ATR-FTIR is suitable to detect the
burn wound healing stages and in the future can be an
auxiliary instrument for clinical routine.
Como referenciar
CASTRO, PEDRO; ZORN, TELMA; ZEZELL, DENISE. Biochemical characterization of skin burn wound healing using ATR-FTIR. In: SBFOTON INTERNATIONAL OPTICS AND PHOTONICS CONFERENCE, October 08-10, 2018, Campinas, SP. Proceedings... Piscataway, NJ, USA: IEEE, 2018. DOI: 10.1109/SBFoton-IOPC.2018.8610943. Disponível em: http://repositorio.ipen.br/handle/123456789/29822. Acesso em: 30 Dec 2025.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.