Characterization of the europium tetracycline complex as a biomarker for atherosclerosis
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2016
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BIOPHOTONICS: PHOTONIC SOLUTIONS FOR BETTER HEALTH CARE, 5th
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Resumo
Atherosclerosis is a narrowing of the arteries caused by an increase of atheromatous plaque: material formed by
macrophage cells containing cholesterol and fatty acids, calcium and a variable amount of fibrous connective tissue. The
elation between vulnerable plaques and cardiovascular events can be determined using plaque biomarkers. In this work,
atherosclerotic plaques stained with different molar ratios of europium, in a potential plaque biomarker, europium
tetracycline complex, were studied by fluorescence microscopy. The tetracycline antibiotic used was chlortetracycline.
The growth of atherosclerotic plaque was followed during 60 days in New Zealand rabbits divided in two groups: an
experimental group (EG), with nine animals and a control group (CG) with three animals. The animals in the EG
received a diet with 1% of cholesterol and the animals of GC received a normal diet. The aortic arch of the animals with
60 days were cut in the vertical plane in 6 μm thick slices, which were mounted on glass slides and stained with
hematoxylin an eosin and europium chlortetracycline complex (EuCTc). The fluorescence images were obtained exciting
the EuCTc absorption band with a filter cube D (BP 355 – 425) and the emission was collected with a LP 470
suppression filter. Light intensity, detector gain and acquisition time were fixed for comparisons. The 20× magnified
images were collected with 12 bit (or 4096 gray tones) resolution. The mean value of gray scale for each molar ratio of
EuCTc was different, indicating that the complex interacts with the components of atherosclerotic plaque and the best
molar ratio was 1.5 EuCTc. These results indicate the potential use of the EuCTc biomarker for atherosclerotic plaque
characterization.
Como referenciar
COURROL, LILIA C.; SILVA, MONICA N. da; SICCHIERI, LETICIA B. Characterization of the europium tetracycline complex as a biomarker for atherosclerosis. In: POPP, JURGEN (ed.); TUCHIN, VALERY (ed.); MATTHEWS, DENNIS L. (ed.); PAVONE, FRANCESCO S. (ed.). In: BIOPHOTONICS: PHOTONIC SOLUTIONS FOR BETTER HEALTH CARE, 5th, April 03, 2016, Brussels, Belgium. Proceedings... p. 98872D-1 - 98872D-7. (SPIE Proceedings Series, 9887). Disponível em: http://repositorio.ipen.br/handle/123456789/27525. Acesso em: 30 Dec 2025.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.