SABRINA METAIRON
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Artigo IPEN-doc 26908 Determination of inorganic elements in blood of mice immunized with Bothrops Snake venom using XRF and NAA2015 - SILVA, L.F.F.L. da; ZAMBONI, C.B.; BAHOVSCHI, V.; METAIRON, S.; SUZUKI, M.F.; SANTANNA, O.A.; RIZZUTTO, M.A.In this work, mice genetically modified [HIII line] were immunized against different Bothrops snake venoms to produce anti-Bothrops serum (antivenom). The Neutron Activation Analysis (NAA) and Energy Dispersive X-Ray Fluorescence (EDXRF) techniques were used to evaluate Ca and Fe concentrations in blood of these immunized mice in order to establish a potential correlation between both phenotypes: antibody response and blood constituents after Bothrops venom administration. The results were compared with the control group (mice not immunized) and with human being estimative. These data are important for clinical screening of patients submitted to immunological therapy as well as the understanding of the envenoming mechanisms.Artigo IPEN-doc 25900 Ions concentration in blood samples of SJL/J dystrophic mice strains using X-ray fluorescence spectrometry2019 - GIOVANNI, D.N.S.; ALMEIDA, M.R.; ZAMBONI, C.B.; METAIRON, S.; BALDUINO, K.N.; SUZUKI, M.F.; BUENO JUNIOR, C.R.Star This study proposes an investigation of ions in whole blood of the dystrophic animal model SJL/J (mice strain with dysferlin protein deficiency) and in the control group (C57BL/6J) using the Energy Dispersive X-ray Fluorescence (EDXRF) spectrometry technique. The comparison between control and dystrophic mice results shown an increase in blood for P, S, K and Fe (p < 0.05) while a decrease in Ca (p < 0.05). This elemental analysis will contribute to evaluate the best diagnostic, care and treatment procedures, for the Progressive Muscular Dystrophy.Artigo IPEN-doc 25194 Evaluation of ions and metals in the blood of GRMD dogs submitted to hASCs therapy by NAA and XRF techniques2019 - METAIRON, SABRINA; ZAMBONI, CIBELE B.; SUZUKI, MIRIAM F.; BUENO, CARLOS R.The elements Br, Ca, Cl, Cr, Fe, K, Mg, Na, P, Rb, S, and Zn were investigated in the whole blood samples of Golden Retriever dogs submitted to cell therapy (hASCs). These analyses were performed over 2 years using Neutron Activation Analysis and X-Ray Fluorescence techniques. The results were compared with control and untreated dog's. A significant increase was observed in K blood levels. There was also variation in blood levels of Br, Cr, Fe, Rb, S, and Zn.Artigo IPEN-doc 24352 Iron determination in whole blood samples of dystrophic mouse strains using X-Ray fluorescence spectrometry2017 - METAIRON, SABRINA; ZAMBONI, CIBELE B.; GIOVANNI, DALTON N. da S.; SUZUKI, MIRIAM F.; BUENO JUNIOR, CARLOS R.; RIZZUTTO, MARCIA de A.Several diseases can be monitored by assessing the variation of specific ion concentration in body fluids. This study proposes an alternative analysis, based on the X-ray fluorescence spectrometry technique, for the investigation of iron (Fe concentration determination) in whole blood of the dystrophic animal models. We performed blood analyses from three different strains with Muscular Dystrophy (Dmdmdx/ J, SJL/J and A/J) and from healthy (C57BL/6J) mice strain adopted as the control group. The results emphasize that iron levels in whole blood male samples of SJL/J and A/J are significantly higher. Related to the gender for Dmdmdx/J and SJL/J mouse strains the comparison emphasizes that iron level increase for males. The study of Fe behavior in whole blood mice samples with muscular dystrophy contributes to its diagnosis, providing a better understanding of this disease.Resumo IPEN-doc 23901 Iron determination in whole blood samples of dystrophic mice strains using X-ray fluorescence spectrometry2017 - SILVA, DALTON G.N. da; METAIRON, SABRINA; ZAMBONI, CIBELE B.; SUZUKI, MIRIAM F.; BUENO JUNIOR, CARLOS R.; RIZZUTTO, MARCIA de A.The use of alternative analytical techniques to investigate specific electrolytes in body fluids has increased in past years with the goal of adding advantages and simplifications, compared to the procedures used in conventional clinical practice. Specifically, in this study, a portable X-ray spectrometer (PXRFS) from Amptek (model X-123 SDD) with Ag X-ray mini-tube has been employed for the whole blood iron measurements in mice species with Muscular Dystrophy (Dmdmdx/J, SJL/J and A/J). The term whole blood refers to solid (cells) and liquid (plasma) components in blood. Usually the conventional procedures for clinical tests are performed in serum using samples of 0.5 to 1.0 mL. However, when the biological material is scarce (case of small size animal model, i.e. mice), the possibility to use whole blood became a fascinating alternative for clinical practice: the Energy Dispersive X-Ray Fluorescence (EDFRX) analyses, using PXRFS, can be done using 50 μL (the total body blood in mice is around 1.0 mL). In this study, we investigated whole blood samples of dystrophic mice strains, a genetic disease that cannot be reversed. In this investigation, twenty samples were investigated. The blood samples were obtained from Jackson Laboratory (Maine, USA) and further inbred at IPEN. The whole blood was collected by the retro–orbital venous plexus and immediately transferred to filter paper (Whatman n41), prior to its coagulation. No anticoagulants or reagents have been used to prevent interference on the results. This procedure simplify the preparation and conservation of biological samples: it is not a destructive procedure and the sample does not need refrigeration. The collection was performing according to the rules approved by Animal Research Ethics Committee (087/99). A comparative study between EDXFX and INAA (Instrumental Neutron Activation Analysis) data was also carried out and the results are in good agreement. This alternative procedure was capable, in a few minutes, to determine whole blood Fe concentrations higher than 6.5 mgL-1, without chemical digestion using direct and non-destructive analysis. The study of Fe behavior in whole blood samples of mice with muscular dystrophy contributes to its diagnosis.Resumo IPEN-doc 23843 Whole blood differences of inorganic elements in dystrophic animal models2016 - METAIRON, S.; ZAMBONI, C.B.; SUZUKI, M.F.The Duchenne Muscular Dystrophy (DMD) disease is characterized by presenting an irreversible progressive degeneration of skeletal muscle. Currently, no effective treatment is available and the DMD research progress depends on animal models. In order to evidence in more details the alterations that DMD disease may cause in blood a comparison of inorganic elements between two animal models (mouse and dog) with muscular dystrophy were made. The dystrophic models were obtained from different research centers at São Paulo city. Several inorganic elements of clinical and nutritional relevance were investigated using Neutron Activation Analysis (NAA). These measurements were performed in the nuclear reactor IEA-R1 (3.5- 4.5MW, pool type) at IPEN/CNEN-SP, Brazil. The knowledge of the elemental composition in these animal models may also help to identify the physiologic difference among them. Moreover, these data can be useful for biochemistry analyses contributing for studying in more details the anomalies caused by DMD.Resumo IPEN-doc 23842 Analytic techniques to assist the antivenom production in Brazil2016 - METAIRON, S.; ZAMBONI, C.B.; SUZUKI, M.F.According to World Health Organization (WHO) the number of deaths by poisonous animals in developed countries is significant. The situation in Brazil has been monitored by SINAN (Sistema de Informação de Agravos e Notificação) and currently 400 accidents occur every month, of which 80% are due to venomous snakebites of the genus Bothrops. To meet the large number of incidences, Instituto Butantan (research center, at Brazil) has produced various types of antivenomous including anti-Bothrops serum. However, the high diversity of snakes (~380 species), a large portion of them being venomous, it can sometimes result of an inappropriate antivenom. In addition, recent investigation showed that the Bothrops venom of certain species can vary significantly according to geographic distribution (in the Northern region the snake poison of jararaca is 30 times more potent than that found in the Southeast region). In this study, the inorganic elements concentration in blood of mice immunized with different species of Bothrops snake venoms were investigated using NAA and XRF techniques. Comparing the results permit to assess whether it is possible to use a single anti-Bothrops serum. Moreover, these results were compared with human estimative to prevent damage due to toxicity of these elements in the immunological therapy.Artigo IPEN-doc 22423 Determination of iron content in whole blood in different mouse strains using a portable XRFS spectrometer2016 - ZAMBONI, CIBELE B.; METAIRON, SABRINA; SUZUKI, MIRIAM F.; BAHOVSCHI, VANESSA; RIZZUTTO, MARCIA A.Artigo IPEN-doc 22408 Inorganic elements in blood of mice immunized with snake venom using NAA and XRF techniques2016 - METAIRON, S.; ZAMBONI, C.B.; SUZUKI, M.F.; SILVA, L.F.F.L. da; RIZZUTTO, M.A.Resumo IPEN-doc 21552 Inorganic elements in blood of mice (HIII) immunized with snake venom using INAA and EDXRF techniques2015 - METAIRON, SABRINA; ZAMBONI, CIBELE; SUZUKI, MIRIAM; SILVA, LAIS L. da; RIZZUTTO, MARCIA
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