Study of 99mTc-DMSA biodistribution in experimental animals
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2017
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INTERNATIONAL NUCLEAR ATLANTIC CONFERENCE
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99mTc-DMSA, succimer (99m Tc), is a radiopharmaceutical commonly used in nuclear medicine for renal
function evaluation by imaging. In order to achieve adequate labeling of the product with good radiochemical
yield and standardized biological distribution, the interval of 185 - 3700 MBq should be kept in a maximum
volume of 3 mL for product labeling. Moreover, one should avoid exposing the reconstituted solution to oxygen
and using the product after four hours post labeling. The aim of the study was to quantify and evaluate the
influence of different DMSA complexes on biological distribution of the radiopharmaceutical in experimental
animals, taking in account variations in the labeling parameters. Radiochemical purity was determined by paper
and thin layer chromatography using both acetone/Whatman 3MM, 0.9% NaCl/TLC-SG and npropanol/
H2O/acetic acid (4:3:1 V/V/V)/TLC-SG systems respectively for quantification of 99mTcO4
- and
99mTcO2 plus some 99mTc-DMSA complexes. The labeling activity did not significantly affect the extent of the
main complex generation. The presence of oxygen and the concentration of 99Tc did not markedly change the
percentage of the radiochemical impurities in the preparation. Radiochemical purity tests of the DMSA-99mTc
based on IPEN-CNEN DMSA-TEC reagent and on another producer’s reagent showed similar results. Although
the routine method used by IPEN-CNEN to determine the radiochemical yield of 99mTc-DMSA was not able to
discriminate among 99mTc-DMSA complexes, the renal uptake and the kidney to liver plus spleen uptake ratio in
rats met the official compendia criteria for the radiopharmaceutical.
Como referenciar
CASTRO, THAIS O.M. de; SILVA, NATANAEL G. da; COLTURATO, MARIA T.; FELGUEIRAS, CARLOS F.; MENGATTI, JAIR; FUKUMORI, NEUZA T.O.; MATSUDA, MARGARETH M.N.; ARAUJO, ELAINE B. de. Study of 99mTc-DMSA biodistribution in experimental animals. In: INTERNATIONAL NUCLEAR ATLANTIC CONFERENCE, October 22-27, 2017, Belo Horizonte, MG. Proceedings... Rio de Janeiro, RJ: Associação Brasileira de Energia Nuclear, 2017. Disponível em: http://repositorio.ipen.br/handle/123456789/28281. Acesso em: 12 May 2024.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.