Effects of near-infrared low level laser irradiation on melanoma cells
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2019
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BRAZILIAN CONGRESS ON BIOMEDICAL ENGINEERING, 26th
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Resumo
Low-level laser (LLL) therapy promotes biostimulating
effects in cell cultures growing in nutritional deficit.
However, the effects of LLLs on tumor cell lines remain
controversial. Studies indicate stimulatory, inhibitory or
even no influence in this type of cells. Therefore, the aim
of this study was to evaluate the influence of LLL
irradiation on the cell viability (with and without
nutritional deficit) of human melanoma SKMEL 37 cells
and murine melanoma B16F10 cells using an infrared
laser (k = 780 nm) with different radiant exposures. The
cell lines were subjected to the LLL 24 h after they were
seeded in a 96-well plate at a density of 5 104 cells per
well. The analysis of cell proliferation by mitochondrial
activity occurred at intervals of 24 and 72 h after laser
irradiation. At each time, culture medium was removed
and 180 μL of PBS and 20 μL of MTT were added. The
plates were incubated for 4 h and the absorbance was read
in a microplate reader at 570 nm. Results showed a
non-significant statistical difference among the groups for
both cell lines regardless the nutritional medium. The
metabolic pattern was similar among the groups. It is
concluded that irradiation with 780 nm laser light at
radiant exposures of 30, 90 and 150 J/cm2 and an output
power of 40 mW does not promote cell proliferation on
melanoma cell lines.
Como referenciar
CONTATORI, CAROLINA G. de S.; SILVA, CAMILA R.; RIBEIRO, MARTHA S. Effects of near-infrared low level laser irradiation on melanoma cells. In: COSTA-FELIX, RODRIGO (ed.); MACHADO, JOÃO C. (ed.); ALVARENGA, ANDRÉ V. (ed.). In: BRAZILIAN CONGRESS ON BIOMEDICAL ENGINEERING, 26th, October 21-25, 2018, Armação de Buzios, RJ. Proceedings... Singapore: Springer Nature Singapore, 2019. p. 797-801. (IFMBE Proceedings, volume 70/2). DOI: 10.1007/978-981-13-2517-5_122. Disponível em: http://repositorio.ipen.br/handle/123456789/30149. Acesso em: 24 Apr 2024.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.