Semi quantitative evaluation of skin burn wound healing
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2019
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ENCONTRO DE OUTONO DA SOCIEDADE BRASILEIRA DE FÍSICA, 42.
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Resumo
The role of skin wound healing the biochemical mechanisms are still not fully understood and spectroscopy may
shine some light on this information. The Fourier Transform Infrared spectroscopy coupled with Attenuated
Total Re°ectance (ATR-FTIR) has been demonstrated potential to monitor the biomolecule activity upon
biological samples. This work aims to evaluate the feasibility of using ATR-FTIR to discriminate burned
skin throughout wound stages. Water vapor at 90±C was applied to the dorsum of Wistar rats resulting in
standardized third-degree burns. Tissue samples were collected after 3±, 7±, 14± and 21 days post burn injury.
For the acquisition of the spectra, 150 scans were averaged with a resolution of 4 cm¡1 and wavenumbers ranging
from 4000 to 400 cm¡1. Analysis of the spectra was calculated using MATLABR°R2015a (MathWorks, Natick,
MA) software. Fingerprint region (900 - 1800 cm¡1) and high wavenumber (2800 - 3000 cm¡1) of spectra were
o®set-corrected and vector normalized. Area under the curve (AUC) of collagen (1236 cm¡1), amide II (1540
cm¡1), amide I (1632 cm¡1) and lipid (2852 cm¡1) of each group were performed by integration method. The
FTIR results exhibit the high biochemical activity of the tissue in the 14o post burn injury when it is compared
with control group which correspond to the stage when the new tissue formation is still ongoing. The lipid
content decrease in the 21 day, which suggest that the metabolic activity and structural reorganization decrease
as the wound healing progress. For future works, micro-FTIR imaging will be used spatially discriminate the
bands.
Como referenciar
CASTRO, PEDRO A.A. de; ZEZELL, DENISE M.; MORAIS, MYCHEL R.P.T.; ZORN, TELMA M. Semi quantitative evaluation of skin burn wound healing: ATR-FTIR study. In: ENCONTRO DE OUTONO DA SOCIEDADE BRASILEIRA DE FÍSICA, 42., 26-31 de maio, 2019, Aracaju, SE. Resumo... São Paulo: Sociedade Brasileira de Física, 2019. Disponível em: http://repositorio.ipen.br/handle/123456789/30218. Acesso em: 26 Apr 2024.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.