Effects of low-level laser irradiation on VEGF expression of melanoma cell lines
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2020
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Lasers in Medical Science
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Impact of low-level laser irradiation on tumor cell lines remains
controversial. Vascular endothelial growth factor (VEGF) is a key
molecule to form new blood vessels, which contribute for cancer
development and growth. The aim of this study was to evaluate
the effects of different light fluences on human melanoma
SKMEL 37 cells and murine melanoma B16F10 cells using a
near infrared laser (λ= 780 nm) with output power of 40 mW
delivering energies of 1.2, 3.6 and 6 J (fluences of 30, 90 and
150 J/cm2, respectively). The cell lines were irradiated 24 h after
they were seeded in a 96-well plate at a density of 5x103 cells
per well, in triplicate at three different days. Following irradiation,
both cell line supernatants were stored in Eppendorf tubes at -
20°C until VEGF-A expression measurement. Specific ELISA kits
were used according to cell line (murine or human). Samples and
standard solutions were added in a 96-well plate antibody-coated
and incuba ted over night at 4°C. Reagent dilution and set time
followed fabricant instructions. The stop solution was added and
the absorbance was read in a microplate reader at 450 nm.
Results showed a non-statistically significant difference among
treated and control groups for both cell lines. These findings
indicate that irradiation with near infrared laser does not influence
VEGF expression on melanoma cell lines regardless the fluence
used and should be tested to prevent cancer growth in preclinical
assays.
Como referenciar
CONTATORI, C.G. de S.; SILVA, C.R.; YOSHIMURA, T.M.; RIBEIRO, M.S. Effects of low-level laser irradiation on VEGF expression of melanoma cell lines. Lasers in Medical Science, v. 35, n. 1, p. 269-269, 2020. DOI: 10.1007/s10103-019-02900-7. Disponível em: http://repositorio.ipen.br/handle/123456789/31110. Acesso em: 24 Apr 2024.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.