RENATA DAMIANI
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Resumo IPEN-doc 24397 Carbohydrate composition and site-occupancy determination in pituitary and recombinant preparations of human thyrotropin2017 - BARTOLINI, PAOLO; RIBELA, MARIA T.C.P.; DAMIANI, RENATA; SILVA, FELIPE D.; LIMA, ELIANA R.; OLIVEIRA, JOAO E.; PERONI, CIBELE N.; TORJESEN, PETER A.; SOARES, CARLOS R.Human thyrotropin (hTSH) is a glycoprotein with three potential glycosylation sites: two in the -subunit and one in the -subunit. Carbohydrate site-occupancy is frequently neglected in glycoprotein characterization, even if related to folding, trafficking, initiation of inflammation, host defence and congenital disorders of glycosylation (CDG). For the first-time N-glycoprofiling analysis was applied to site-occupancy determination of two native pituitary hTSH, in comparison with three CHO-derived preparations of hTSH, a widely used biopharmaceutical. A single methodology provided: (i) average N-glycan mass; (ii) mass fraction of each monosaccharide and of sulfate; (iii) percent carbohydrate. The results indicate that occupancy (65–87%) and carbohydrate mass (12–19%) can be 34–57% higher in recombinant hormones. The average glycan mass is 24% lower in pituitary hTSH and contains ∼3-fold fewer moles of galactose (P < 0.005) and sialic acid (P < 0.01). The number of moles of fucose per mole of hTSH was found 2.5-fold higher in the pituitary preparations. One of these native preparations, presenting the smallest glycan mass, lowest occupancy, GalNAc, sulfate, Gal and sialic acid contents, also presented the lowest in vivo bioactivity and circulatory half-life. This methodology, extremely important for comparing a recombinant biopharmaceutical to its native equivalent, can be applied to any physiologically or clinical relevant glycoprotein.Artigo IPEN-doc 22995 N-Glycoprofiling analysis for carbohydrate composition and site-occupancy determination in a poly-glycosylated protein: human thyrotropin of different origins2017 - RIBELA, MARIA T.C.P.; DAMIANI, RENATA; SILVA, FELIPE D.; LIMA, ELIANA R.; OLIVEIRA, JOAO E.; PERONI, CIBELE N.; TORJESEN, PETER A.; SOARES, CARLOS R.; BARTOLINI, PAOLOHuman thyrotropin (hTSH) is a glycoprotein with three potential glycosylation sites: two in the -subunit and one in the -subunit. These sites are not always occupied and occupancy is frequently neglected in glycoprotein characterization, even though it is related to folding, trafficking, initiation of inflammation and host defense, as well as congenital disorders of glycosylation (CDG). For the first time N-glycoprofiling analysis was applied to the site-occupancy determination of two native pituitary hTSH, in comparison with three recombinant preparations of hTSH, a widely used biopharmaceutical. A single methodology provided the: (i) average N-glycan mass; (ii) mass fraction of each monosaccharide and of sulfate; and (iii) percent carbohydrate. The results indicate that the occupancy (65%–87%) and carbohydrate mass (12%–19%) can be up to 34%–57% higher in recombinant hormones. The average glycan mass is 24% lower in pituitary hTSH and contains ~3-fold fewer moles of galactose (p < 0.005) and sialic acid (p < 0.01). One of the two native preparations, which had the smallest glycan mass together with the lowest occupancy and GalNAc, sulfate, Gal and sialic acid contents, also presented the lowest in vivo bioactivity and circulatory half-life. The methodology described, comparing a recombinant biopharmaceutical to its native equivalent, can be applied to any physiologically or clinical relevant glycoprotein.Artigo IPEN-doc 20704 N-glycoprofiling analysis in a single glycoprotein model: a comparison between recombinant and pituitary glycosylated human prolactin2015 - CAPONE, MARCOS V.N.; SUZUKI, MIRIAM F.; OLIVEIRA, JOAO E.; DAMIANI, RENATA; SOARES, CARLOS R.J.; BARTOLINI, PAOLOResumo IPEN-doc 20256 Effects of butyrate and manganese on productivity, sialylation, N-glycosylation site occupancy and biological properties of CHO-derived thyrotropin2014 - DAMIANI, RENATA; OLIVEIRA, JOAO E.; ALMEIDA, BEATRIZ E.; SANT'ANA, PATRICIA M.; DALMORA, SERGIO L.; BARTOLINI, PAOLO; RIBELA, MARIA T.C.P.Resumo IPEN-doc 11433 Comparative studies of pituitary (NIDDK, USA) and recombinant (Thyrogen and IPEN) human thyroid stimulating hormone (hTSH) for what concerns cabohydrate structures and charge heterogeneity2006 - OLIVEIRA, J.E.; LOUREIRO, R.F.; CARVALHO, C.M.; DAMIANI, R.; BARTOLINI, P.; RIBELA, M.T.C.P.Resumo IPEN-doc 16586 RP-HPLC qualitative and quantitative analysis of glycoprotein hormones in the presence of high amounts of human serum albumin: hLH, hCG, hFSH and hTSH2011 - ALMEIDA, B.E.; OLIVEIRA, J.E.; DAMIANI, R.; DALMORA, S.L.; BARTOLINI, P.; RIBELA, M.T.C.P.Resumo IPEN-doc 16585 First expression of the antagonist of mouse prolactin (S177D-mPRL) by adherent dhfr-CHO cell using p658 vector2011 - SUZUKI, M.F.; ARTHUSO, F.S.; OLIVEIRA, J.E.; CAPONE, M.V.; DAMIANI, R.; OLIVEIRA, N.A.J.; GOULART RODRIGUES, H.; RIBELA, M.T.C.P.; SOARES, C.R.J.; BARTOLINI, P.Resumo IPEN-doc 16441 Glycosylation and pharmacokinetics of human thyrotropin2011 - DAMIANI, R.; OLIVEIRA, J.E.; ALMEIDA, B.E.; VORAUER-UHL, K.; BARTOLINI, P.; RIBELA, M.T.C.P.Resumo IPEN-doc 16160 Charge isomer distribution of different pituitary and recombinant human thyrotropin (hTSH) preparations2010 - DAMIANI, R.; OLIVEIRA, J.E.; ALMEIDA, B.E.; BARTOLINI, P.; RIBELA, M.T.C.P.Resumo IPEN-doc 16159 International collaborative study to establish the 2nd WHO International Standard of Human Recombinant Fillicle Stimulating Hormone for Bioassai2010 - OLIVEIRA, J.E.; ALMEIDA, B.E.; DAMIANI, R.; BARTOLINI, P.; RIBELA, M.T.C.P.The aim of the International Collaborative Study proposed by the National Institute for Biological Standards and Control (WHO) is to calibrate a new preparation of human follicle stimulating hormone (hFSH) relative to the 1st International Standard for bioassay of recombinant-hFSH (WHO 92/642), which is almost exhausted and requires to be replaced. The candidate preparation and four other samples, which correspond to this preparation stored at elevated temperatures (K, L, M, N), were analyzed qualitatively and quantitatively in our laboratory, by reversed-phase high-performance liquid chromatography (RPHPLC). The candidate content determined in six assays (8.59 ± 0.41 μg/ampoule) was 20% lower, when compared with the current standard. Based on this, an average value of 110 IU FSH per ampoule could be assigned to this preparation. The main isoform of the candidate preparation showed a lower retention time (22.5 ± 0.83 min) relative to WHO 92/642 (22.8 ± 0.81 min) and also to an internal reference preparation (22.6 ± 0.86 min). These differences, though, were not significant (p>0.05). Practically identical contents were also determined for K, L, M and N ampoules (8.44 ± 0.20; 8.56 ± 0.33; 8.57 ± 0.38; 8.69 ± 0.30 μg/ampoule, respectively). No significant differences in FSH retention times between the candidate preparation and the ampoules stored at elevated temperatures were observed.