In this study, ions of clinical relevance in non-stimulated human whole saliva
obtained from healthy subject’s donors (adults and children) at São Paulo city (Brazil),
were investigated. The Instrument Neutron Activation Analysis (INAA) and Energy
Dispersive X-Ray Fluorescence (EDXRF) techniques were used. The comparison
concentration between adults and children for Cl, K, Ca and Fe showed significant
differences for all elements, emphasizing the need of adopting different reference
values.