BEATRIZ ELANE DE ALMEIDA

BEATRIZ ELANE DE ALMEIDA

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Effects of butyrate and manganese on productivity, sialylation, N-glycosylation site occupancy and biological properties of CHO-derived thyrotropin
2014 - DAMIANI, RENATA; OLIVEIRA, JOAO E.; ALMEIDA, BEATRIZ E.; SANT'ANA, PATRICIA M.; DALMORA, SERGIO L.; BARTOLINI, PAOLO; RIBELA, MARIA T.C.P.
RP-HPLC qualitative and quantitative analysis of glycoprotein hormones in the presence of high amounts of human serum albumin: hLH, hCG, hFSH and hTSH
2011 - ALMEIDA, B.E.; OLIVEIRA, J.E.; DAMIANI, R.; DALMORA, S.L.; BARTOLINI, P.; RIBELA, M.T.C.P.
Glycosylation and pharmacokinetics of human thyrotropin
2011 - DAMIANI, R.; OLIVEIRA, J.E.; ALMEIDA, B.E.; VORAUER-UHL, K.; BARTOLINI, P.; RIBELA, M.T.C.P.
Charge isomer distribution of different pituitary and recombinant human thyrotropin (hTSH) preparations
2010 - DAMIANI, R.; OLIVEIRA, J.E.; ALMEIDA, B.E.; BARTOLINI, P.; RIBELA, M.T.C.P.
International collaborative study to establish the 2nd WHO International Standard of Human Recombinant Fillicle Stimulating Hormone for Bioassai
2010 - OLIVEIRA, J.E.; ALMEIDA, B.E.; DAMIANI, R.; BARTOLINI, P.; RIBELA, M.T.C.P.
The aim of the International Collaborative Study proposed by the National Institute for Biological Standards and Control (WHO) is to calibrate a new preparation of human follicle stimulating hormone (hFSH) relative to the 1st International Standard for bioassay of recombinant-hFSH (WHO 92/642), which is almost exhausted and requires to be replaced. The candidate preparation and four other samples, which correspond to this preparation stored at elevated temperatures (K, L, M, N), were analyzed qualitatively and quantitatively in our laboratory, by reversed-phase high-performance liquid chromatography (RPHPLC). The candidate content determined in six assays (8.59 ± 0.41 μg/ampoule) was 20% lower, when compared with the current standard. Based on this, an average value of 110 IU FSH per ampoule could be assigned to this preparation. The main isoform of the candidate preparation showed a lower retention time (22.5 ± 0.83 min) relative to WHO 92/642 (22.8 ± 0.81 min) and also to an internal reference preparation (22.6 ± 0.86 min). These differences, though, were not significant (p>0.05). Practically identical contents were also determined for K, L, M and N ampoules (8.44 ± 0.20; 8.56 ± 0.33; 8.57 ± 0.38; 8.69 ± 0.30 μg/ampoule, respectively). No significant differences in FSH retention times between the candidate preparation and the ampoules stored at elevated temperatures were observed.
Physico-chemical quality control of human chrionic gonadotropin preparations
2010 - ALMEIDA, B.E.; OLIVEIRA, J.E.; DAMIANI, R.; DALMORA, S.L.; BARTOLINI, P.; RIBELA, M.T.C.P.
Reversed-phase higg-performance liquid chromatography characterization of human menopausal gonadotropin preparations
2010 - ALMEIDA, B.E.; OLIVEIRA, J.E.; DAMIANI, R.; SPIGOTI, G.; DALMORA, S.L.; BARTOLINI, P.; RIBELA, M.T.C.P.
Sodium butyrate greatly enhances human thyrotropin (hTSH) synthesis by serum-free CHO cell culture with alterations of the carbohydrate moiety
2012 - DAMIANI, R.; OLIVEIRA, J.E.; ALMEIDA, B.E.; BARTOLINI, P.; RIBELA, M.T.C.P.
Quantitative analysis of different preparations of human thyrotropin (hTSH): a comparison between RP-HPLC and the in vivo bioassay based on thyroxine stimulaton mice
2012 - ALMEIDA, B.E.; DAMIANI, R.; OLIVEIRA, J.E.; RIBELA, M.T.C.P.; BARTOLINI, P.
Physico-chemical characterization of alpha and beta subunit of recombinant human glycoprotein hormones: hTSH and hLH
2007 - CARVALHO, CRISTIANE M.; OLIVEIRA, JOAO E.; DAMIANI, RENATA; ALMEIDA, BEATRIZ E.; CECCHI, CLAUDIA R.; BARTOLINI, PAOLO; RIBELA, MARIA T.C.P.