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Resumo IPEN-doc 26936 Estudo da liberação de clorexidina incorporada em nanopartículas de montmorilonita em compósitos a base de BisGMA/TEGDMA2016 - CAMPOS, L.M.P.; BOARO, L.C.C.; SANTOS, T.M.R.; VARCA, G.H.C.; SANTOS, L.K.G.; LUGAO, A.B.; PARRA, D.F.O objetivo desse estudo foi desenvolver nanocompósitos experimentais adicionados com nanopartículas de Montmorilonita (MMT) incorporadas com diacetato de clorexidina (CHX) como carga, em uma matriz polimérica a base de BisGMA/TEGDMA (1:1). Foram confeccionados compósitos experimentais (n=5) adicionados com carga nas concentrações de 0.01, 0.5, 2 e 10% em massa (p/p). A liberação da CHX foi avaliada in vitro em uma solução neutra (pH 7, 50mm) a 37 oC, por 366 horas. A quantificação de CHX foi avaliada por espectrofotometria de UV (λ=255 nm). A formação de nanocompósitos foi avaliada por meio das metodologias Microscopia Eletrônica de Transmissão (MET) e Difração de Raio-X (DRX). Foi observada uma liberação de fármaco constante em todos os grupos analisados, de 0 a 366 horas. Ao atingir 366 horas, o grupo adicionado com 0,01% de carga apresentou 0.126 g.L-1 de liberação de CHX, o grupo com 0.5% apresentou 0.139 g.L-1, o grupo com 2% apresentou 0.150 g.L-1 e o grupo com 10% apresentou 0.151 g.L-1. Observou-se por meio do DRX e MET que houve intercalação entre a MMT (adicionada com CHX) e a matriz polimérica, em todos os grupos avaliados. Concluiu-se que houve a formação de nanocompósitos e que os mesmos promoveram constante liberação de CHX entre 0 a 366 horas. Esses resultados evidenciaram um forte potencial no desenvolvimento de nanocompósitos contendo fármaco antimicrobiano para aplicação odontológica.Resumo IPEN-doc 24966 Conversion of endodontic sealer modified with nanoparticles carrying antibacterial drugs2017 - SANCHES, L.K.F.; CAMPOS, L.M.P.; PARRA, D.F.; BRAGA, R.R.; SANTOS, M.; BOARO, L.C.C.Purpose/aim: The aim of this study was to evaluate the polymeric conversion of a commercial endodontic sealer modified with montmorillonite nanoparticles carrying to different antibacterial drugs: clorhexidine (CHX) and metronidazole (MET). Materials and methods: The sealer used was AH Plus sealer, and 5% in weight was added of the nanoparticles carrying one of the drugs CHX or MET. Cylindrical specimens were made with 5 mm diameter and 1 mm thick (n = 5). The degree of conversion (DC) was determined using near FTIR. DC was evaluated using the absorption peak at 4529 cm−1 that decrease during polymerization, and the absorption peak at 4623 cm−1 which is unaltered as normalization. The measurements were obtained for each sample immediately, 4 h and 24 h after mixing the two pastes of the sealer. A group with no modification by the nanoparticle was added as a control. The data was analyzed using one-way ANOVA/Tukey (α = 5%). Results: Results are presented in Table 1. Table 1. Means (SD) for degree of conversion (%) at 4 and 24 h. No statistical difference was presented among groups for neither periods of evaluation. Drug DC (%) 4 h 24 h Control 35 (11) A 68 (8) A CHX 31 (11) A 81 (11) A MET 38 (6) A 77 (14) A Conclusions: Within the limitations of this study it can be concluded that addition of the nanoparticle carrying both drug did not influence the polymerization of the sealer.Resumo IPEN-doc 24964 Antibacterial activity of endodontic sealer modified with nanoparticles2017 - SILVA, L.T.S.; COGO-MULLER, K.; BRANDT, W.C.; CAMPOS, L.M.P.; PARRA, D.F.; SANTOS, M.; BOARO, L.C.C.Purpose/aim: The aim of this study was to evaluate the antibacterial activity of a commercial endodontic sealer modified with montmorillonite nanoparticles carrying to different drugs: clorhexidine (CHX) and metronidazole (MET). Materials and methods: The sealer used was AH Plus sealer, and 5% in weight was added of the nanoparticles carrying one of the drugs CHX or MET. Cylindrical specimens were made with 5 mm diameter and 1 mm thick (n = 5). The antibacterial activity was evaluated by the inhibition halo test. This method was conducted according to Clinical Standard Laboratory Institute M2-A8 protocol, with some modifications (CLSI, 2003). The bacteria selected were the E. Fecallis which is the most frequently bacteria found in recidivate apical infection. Discs specimens were positioned over the agar and plates were incubated in the appropriate conditions for 48 h. After this period, inhibition zones were measured using a calliper rule. A group with no modification by the nanoparticle was added as a control. The data was analyzed using Mann–Whitney (alpha = 5%). Results: Results are presented in Table 1. For the control group no inhibition zone was formed, so the statistical analyze was performed only between the experimental groups. Table 1. Means (SD) for inhibition halo (mm). No statistical difference was presented between the drugs. Drug Inhibition halo (mm) Control 0 CHX 4.8 (1.4) A MET 4.0 (1.6) A Conclusions: Within the limitations of this study it can be concluded that the addition of the nanoparticle carrying both drugs added an antibacterial activity that was not found in the commercial sealer.Resumo IPEN-doc 23686 BisGMA/TEGDMA based material with antibacterial activity2016 - BOARO, L.; CAMPOS, L.M.; VARCA, G.H.C.; MARQUES, P.A.; PIMENTEL, A.C.; ROMAN-TORRES, C.V.; BRANDT, W.C.; COGO-MULLER, K.; BRAGA, R.R.; PARRA, D.F.