SHEILA CYNTHIA GOUW-SOARES

SHEILA CYNTHIA GOUW-SOARES

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Effect of a diode laser irradiation in root canals contamined with Enterococcus faecalis
2002 - RADAELLI, C.R. de M.; ANTUNES, A.; CAI, S.; ZEZELL, D.M.; GOUW-SOARES, S.
High success rates were achieved in conventional endodontic treatment of vital pulp teelh. However, in non-vital pulp a decrease of success occurs due to difficulties to achieve a complete disinfection of root canal system. Some bacteria, such as Enterococcus faecalis, are frequently found in endodontic treatment failure due to their high resistance to the usual process of canal shaping and cleaning. The purpose of this work was to evaluate the effectiveness of a diode laser irradiation in bacterial reduction of contaminated canals as well as to analyze the effect of the laser irradiation associated with the use of calcium hydroxide paste. Eighty two root canals infected with Enterococcus faecalis suspension (1x10(6) CFU/ml) were irradiated with a high power diode laser (810nm, Opus 10- Sharplan - Israel) with two different parameters: 2.5W and 3 W (cw), using a 360 μm fiber at an angle of approximately 5 degrees to the dentine surface during 5 seconds in 5 applications, within 20 seconds intervals between. Temperature was monitored at apex using a K-pipe thermocouple. Maximum temperature was 7.45ºC. After these proceedings specimens were vortexed in peptone water and dilutions performed. Aliquots were plated on m-Enterococcus agar. Colonies Forming Units (CFU) of all groups was counted. Statistically the results showed a significant reduction of bacteria on all groups after laser irradiation. A high reduction rate was achieved: 98.5% immediately after laser irradiation; 48 hours after, the reduction rate was 96.73% and finally a 100% reduction was achieved with the association of laser irradiation and calcium hydroxide paste. These high rates of bacteria reduction were achieved using the parameter of 3W (cw), 4.24 KW/cm2. The use of high power diode laser 810nm is highly efficient and suitable to reduce Enterococcus faecalis in infected root canals.
Comparative study of dentine permeability after apicectomy and surface treatment with 9.6 μm CO2 and Er:YAG laser irradiation
2002 - GOUW-SOARES, S.; STABHOLZ, A.; LAGE-MARQUES, J.L.; ZEZELL, D.M.; EDUARDO, C.P.
Failure of the apicectomies is generally attributed to dentine surface permeability and to the lack of an adequate marginal sealing of the retrofilling material which allow the percolation of microorganisms and their products from the root canal system to the periodontal region compromising the periapical healing. The purpose of this study was to evaluate the dentine and the marginal permeability after apicectomy and surface treatment with 9.6μm CO2 laser and 2.94μm Er:YAG laser irradiation. Sixty-five single rooted human endodontically treated teeth were divided in five experimental groups: group I (control), the apicectomy performed with high speed bur; group II, similar procedure to the group I followed by dentinal surface treatment with the 9.6μm CO2 laser; group III, similar procedure to group 1 followed by dentinal surface treatment with the Er:YAG laser; group IV, apicectomy and surface treatment with 9.6μm CO2 laser, and the group V, apicectomy and surface treatment with the Er:YAG laser. The analysis of methylene blue dye infiltration through the dentinal surface and the retrofilling material demonstrated that the samples from the groups which were irradiated with the lasers showed significantly less infiltration indexes than the ones from the control group. These results were compatible to the structural morphological changes evidenced through the SEM analysis. The samples from the groups II and IV (9.6μm CO2 laser) showed clean smooth surfaces, fusion and recrystallized dentine distributed homogeneously throughout the irradiated area sealing the dentinal tubules. The samples from the groups III and V (Er:YAG laser 2.94μm) also presented clean surfaces, without smear layer but slightly rough compatible to the ablationed dentine and without evidence of dentinal tubules. Through the conditions of this study, the Er:YAG 2.94μm and the 9.6μm CO2 laser used for root canal resection and dentine surface treatment showed a reduction of permeability to methylene blue dye.
Treatment of oral mucositis induced by ionizing radiation in hamsters with low power laser
2008 - GALLETTA, VIVIAN; CORREA, MELISSA F.; CORREA, L.; GOUW SOARES, SHEILA; ZEZELL, D.M.
Preparo cavitario in vitro com laser de Er:YAG. Estudo em microscopia eletronica de varredura
1997 - TANJI, E.Y.; EDUARDO, C.P.; HAYPEK, P.; SOARES, S.C.G.; ZEZELL, D.M.
Analise do comportamento termico do laser de diodo no esmalte, dentina e cemento dental - estudo in vitro
2002 - PELINO, J.E.P.; HAYPEK, P.; SOARES, S.C.G.; BACHMANN, L.; ZEZELL, D.M.; EDUARDO, C.P.
Monitoramento da variação da temperatura na desinfecção intracanal com laser de diodo
2002 - RADAELLI, C.M.; PEREIRA, A.A.; ZEZELL, D.M.; SOARES, S.C.G.
0 uso de Lasers como coadjuvantes na terapêutica endodôntica está consagrado na literatura por diversos pesquisadores. Diversas são as aplicações, dentre elas, destaca-se o alto potencial na vaporização de restos teciduais, de smear layer, e os altos índices de redução microbiana no sistema. de canais radiculares. Diversos estudos, da aplicação dos lasers em endodontia foram realizados com os lasers de Nd:YAG, Ho:YAG, Er:YAG e outros, porém poucos com o laser de diodo. 0 laser de diodo é um laser promissor por ser um aparelho portátil, sendo facilmente transportável, com alto poder de penetração, permitindo desinfecção em profundidade, o que o torna altamente atrativo. Nosso objetivo foi estabelecer parâmetros seguros para seu uso. sem provocar um aumento de temperatura lesivo, a elevação da temperatura na regido crementária não deve ser acima de 10(SUB circ)C. O controle do aumento da temperatura foi realizado com o uso de um termopar do tipo T, constituído por uma liga de cobre-costantan. com 130mm. O dispositivo foi fixado ao cemento na região de 1/3 apical e pasta térmica foi aplicada entre o cemento e o termopar. produzindo maior contato térmico entre o cemento e a ponta do termopar. O terço apical permaneceu em um banho térmico entre 36 e 37°C. Os parâmetros do laser utilizados permitiram verificar um índice satisfatório de redução microbiana e també m estimar parâmetros adequados para a nao ocorrência de lesões devido a efeitos térmicos.
Estudo in vitro de parametros seguros de irradiacao do laser de diodo de alta intensidade no tratamento de condutos radiculares
2002 - RADAELLI, C.A.R.M.; PEREIRA, A.A.; CAI, S.; ZEZELL, D.M.; SOARES, S.C.G.
Er:YAG laser cavity preparation with variable pulsewidth: a microleakage study
2000 - NAVARRO, R.S.; SOARES, S.C.G.; HAYPEK, P.; ZEZELL, D.M.; YOUSSEF, M.N.; EDUARDO, C.P.
Adhesion of fibroblasts cells on dentine cut surfaces by Er:YAG laser treated or not by Nd:YAG laser
2006 - GOUW SOARES, S.; LOBATO, A.; MARQUES, M.; NOGUEIRA, G.E.C.; PELINO, J.E.P.; ZEZELL, D.M.
Gingivoplastía: Informe de 3 casos con láser diodo
2013 - TEIXEIRA, L.R.; AZEVEDO, L.H.; TEIXEIRA, B.C.; GOUW SOARES, S.; ZEZELL, DENISE M.