FERNANDO BACCI EFFENBERGER
3 resultados
Resultados de Busca
Agora exibindo 1 - 3 de 3
Resumo IPEN-doc 31001 Enhancing Rhizobium performance in common bean plants through Fe3O4 nanoparticles2024 - MASUNAGA, SUELI H.; SOUZA-TORRES, ANGEL de; GOVEA-ALCAIDE, ERNESTO; GOMES-PADILLA, ERNESTO; EFFENBERGER, FERNANDO B.; ROSSI, LIANE M.; LOPEZ-SANCHEZ, RAUL; JARDIM, RENATO de F.This study investigates the effects of Fe3O4 nanoparticles (NPs) on nodulation, nitrogen fixation, and growth of common bean plants. Plants were exposed to Fe3O4 NPs, Rhizobium inoculation, and Fe3O4 NPs + Rhizobium inoculation. Results showed that treated plants exhibited improved symbiotic performance, with increased nitrogenase activity, nodule leghaemoglobin, and iron content. Additionally, the number of active nodules per plant and nodule dry weight significantly increased. Symbiotic nitrogen fixation was enhanced, leading to higher shoot and root total nitrogen content. Treatment combining Fe3O4 NPs and Rhizobium inoculation yielded the best results. Fe3O4 NPs were taken up by plants, accumulating in organs including nodules. Moreover, treated plants displayed increased root and shoot lengths, leaf area, and dry weights. Magnetization curves indicated the accumulation of Fe3O4 NPs in nodules, enhancing symbiotic performance. The study suggests a novel strategy for improving common bean growth through Fe3O4 NPs and Rhizobium inoculation, offering potential benefits for sustainable agriculture, and reducing nitrogen fertilizer use. These findings contribute to a non-genetic approach in legume research, emphasizing long-term improvements in common bean growth and symbiosis [1-2].Artigo IPEN-doc 30763 Impacts of Rhizobium inoculation and Fe3O4 nanoparticles on common beans plants2024 - GOVEA-ALCAIDE, E.; DESOUZA, A.; GOMEZ-PADILLA, E.; MASUNAGA, S.H.; EFFENBERGER, F.B.; ROSSI, L.M.; LOPEZ-SANCHEZ, R.; JARDIM, R.F.We have carried out a systematic investigation on the impact of Fe3O4 nanoparticles (NPs) and Rhizobium inoculation on nodulation and growth of common bean plants (cv. Red Guama, Phaseolus vulgaris). Three distinct treatments were conducted on the common bean plants: (i) exposure to Fe3O4 NPs; (ii) Rhizobium inoculation; and (iii) a combined treatment involving Fe3O4 NPs + Rhizobium inoculation, with non-treated plants as controls. Temperature and magnetic field dependence of magnetization, M(T, H), measurements were performed on both the soil, and dried organs of the plants including roots, nodules, stems, and leaves. M(T, H) analyses indicated a systematic increase in magnetization across organs of plants treated with Fe3O4 NPs and combined Fe3O4 NPs + Rhizobium. We have found the magnetic contribution, generally related to Fe content in the soil and plant organs, significantly increased in plants exposed to Fe3O4 NPs, further indicating absorption, translocation, and accumulation of Fe3O4 NPs in the areal parts of the plants. Plants treated with Fe3O4 NPs and combined Fe3O4 NPs + Rhizobium exhibited Fe3O4 NPs accumulation in all organs with increasing concentrations of 69.7 to 74.1 NNPs/g in roots, 5.6 to 7.7 NNPs/g in stems, and 3.1 to 5.5 NNPs/g in leaves, respectively. The iron concentration in nodules was found to be close to 65 NNPs/g. No appreciable difference in the absorption index AI of roots between plants treated with Fe3O4 NPs (~ 1.73%) and Fe3O4 NPs + Rhizobium (~ 1.79%) has been observed. The translocation index TI increased by ~ 46% in plants treated with Fe3O4 NPs + Rhizobium (6.9%) compared to Fe3O4 NPs (4.3%). Treated plants showed improved symbiotic performance including nodule leghaemoglobin and iron content, number of active nodules per plant, and nodule dry weight. The best result was obtained using the combined treatment of Fe3O4 NPs + Rhizobium. This study provides evidence that M(T,H) measurements constitute a valuable tool in monitoring the uptake, translocation, and accumulation of Fe3O4 NPs in plant organs of common bean plants.Artigo IPEN-doc 28161 Crystalline and magnetic properties of CoO nanoparticles locally investigated by using radioactive indium tracer2021 - SANTOS, RENATA V.; CABRERA-PASCA, GABRIEL A.; COSTA, CLEIDILANE S.; BOSCH-SANTOS, BRIANNA; OTUBO, LARISSA; PEREIRA, LUCIANO F.D.; CORREA, BRUNO S.; EFFENBERGER, FERNANDO B.; BURIMOVA, ANASTASIA; FREITAS, RAFAEL S.; CARBONARI, ARTUR W.We herein report a comprehensive investigation on the magnetic, structural, and electric properties of CoO nanoparticles with different sizes by local inspection through hyperfine interactions measured in a wide range of temperatures (10–670 K) by using radioactive 111In(111Cd) tracers with the perturbed angular correlations technique. Small cobalt oxide nanoparticles with the characteristic size of 6.5 nm have been prepared by the wet chemical route that turned out to be essential to incorporate radioactivity tracers during nucleation and growth of the particles. Nanocrystalline samples with 22.1 nm size were obtained by thermal treatments under low pressure of helium at 670 K. The hyperfine data were correlated with X-ray diffraction, ZFC–FC magnetic measurements, and transmission electron microscopy to describe the structure, magnetic properties, size, and shape of samples. An analysis of the temperature evolution of hyperfine parameters revealed that the structural distortion and the magnetic disorder in the core and on the surface layer play an important role in the magnetic behavior of CoO nanoparticles.