LENITA DE FREITAS TALLARICO
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Artigo IPEN-doc 20271 Developmental toxicity, acute toxicity and mutagenicity testing in freshwater snails Biomphalaria glabrata (Mollusca: Gastropoda) exposed to chromium and water samples2014 - TALLARICO, LENITA de F.; BORRELY, SUELI I.; HAMADA, NATALIA; GRAZEFFE, VANESSA S.; OHLWEILER, FERNANDA P.; OKAZAKI, KAYO; GRANATELLI, AMANDA T.; PEREIRA, IVANA W.; PEREIRA, CARLOS A. de B.A protocol combining acute toxicity, developmental toxicity and mutagenicity analysis in freshwater snail Biomphalaria glabrata for application in ecotoxicological studies is described. For acute toxicity testing, LC50 and EC50 values were determined; dominant lethal mutations induction was the endpoint for mutagenicity analysis. Reference toxicant potassium dichromate (K2Cr2O7) was used to characterize B. glabrata sensitivity for toxicity and cyclophosphamide to mutagenicity testing purposes. Compared to other relevant freshwater species, B. glabrata showed high sensitivity: the lowest EC50 value was obtained with embryos at veliger stage (5.76 mg/L). To assess the model applicability for environmental studies, influent and effluent water samples from a wastewater treatment plant were evaluated. Gastropod sensitivity was assessed in comparison to the standardized bioassay with Daphnia similis exposed to the same water samples. Sampling sites identified as toxic to daphnids were also detected by snails, showing a qualitatively similar sensitivity suggesting that B. glabrata is a suitable test species for freshwater monitoring. Holding procedures and protocols implemented for toxicity and developmental bioassays showed to be in compliance with international standards for intra-laboratory precision. Thereby, we are proposing this system for application in ecotoxicological studies.Resumo IPEN-doc 16432 Evaluation of toxic and mutagenic potential of water samples of Tietê river (Suzano, SP, Brazil) in Biomphalaria glabrata (SAY, 1818)2008 - TALLARICO, LENITA de F.; GRAZEFFE, VANESSA S.; OKAZAKI, KAYO; OHLWEILER, FERNANDA P.; KAWANO, TOSHIE; PEREIRA, CARLOS A. de B.; NAKANO, ELIANAA wide range of industrial and domestic wastes are discharged in the River Tieté in the region of Suzano and the biological treatment of effluents is carried out by Wastewater Treatment Plant of SABESP (WWTP Suzano). The samples were collected and transported by SABESP (Companhia de Sancamento Bésico do Estado de Sio Paulo). The following points were chosen: PO - Ponte Nova Dam, P1 — 200 meters upstream to the plant-, P2— the affluent of plant, P3 — the effluent treated by plant before discharge in the river and P4 — 200 meters from the discharge in the river—The samples were evaluated by analyzing acute toxicity and dominant lethal test was performed for detection of germ cell mutations in freshwater snails Biomphalaria glabrata. Acute toxicity assays were conducted in adult specimens and embryo at blastulae, gastrulae, trocophore and veliger stages. Four sampling were performed. In samples at august 2006 and august 2008, the station affluent was toxic for embryos and adults. The results were similar, with LC50 values; 43,04%, 41,56%, 57,16% and 60,06% for embryos at blastulae, gastrulae, trocophore and veliger stages respectively and 100% for adult snails in first sample and 48,24%, 43,71%, 55,43% and 62,64% for embryos at blastulae, gastrulae, trocophore and veliger stages respectively and 84,16% for adult snails in august 2008. Sampling at february 2007 was toxic only for adult snails, with a LC,, value of 41,25% and sampling at february 2008 was not toxic for adults and embryos. In all samplings, after discharge into the river, treated effluents were not toxic for B. glabrata adults and embryos. There was no mutagenicity in all samples. In these study, the potential impact of effluent discharge to the biota of Tieté River was showed. These results show the importance of the WWTP biological treatment of effluents in reducing acute toxicity.Artigo IPEN-doc 13194 Establishment of the comet assay in the freshwater snail Biomphalaria glabrata (Say, 1818)2008 - GRAZEFFE, VANESSA S.; TALLARICO, LENITA de F.; PINHEIRO, ALESSANDRO de S.; KAWANO, TOSHIE; SUZUKI, MIRIAM F.; OKAZAKI, KAYO; PEREIRA, CARLOS A. de B.; NAKANO, ELIANA