CARLOS ALBERTO DE BRAGANÇA PEREIRA
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Artigo IPEN-doc 20271 Developmental toxicity, acute toxicity and mutagenicity testing in freshwater snails Biomphalaria glabrata (Mollusca: Gastropoda) exposed to chromium and water samples2014 - TALLARICO, LENITA de F.; BORRELY, SUELI I.; HAMADA, NATALIA; GRAZEFFE, VANESSA S.; OHLWEILER, FERNANDA P.; OKAZAKI, KAYO; GRANATELLI, AMANDA T.; PEREIRA, IVANA W.; PEREIRA, CARLOS A. de B.A protocol combining acute toxicity, developmental toxicity and mutagenicity analysis in freshwater snail Biomphalaria glabrata for application in ecotoxicological studies is described. For acute toxicity testing, LC50 and EC50 values were determined; dominant lethal mutations induction was the endpoint for mutagenicity analysis. Reference toxicant potassium dichromate (K2Cr2O7) was used to characterize B. glabrata sensitivity for toxicity and cyclophosphamide to mutagenicity testing purposes. Compared to other relevant freshwater species, B. glabrata showed high sensitivity: the lowest EC50 value was obtained with embryos at veliger stage (5.76 mg/L). To assess the model applicability for environmental studies, influent and effluent water samples from a wastewater treatment plant were evaluated. Gastropod sensitivity was assessed in comparison to the standardized bioassay with Daphnia similis exposed to the same water samples. Sampling sites identified as toxic to daphnids were also detected by snails, showing a qualitatively similar sensitivity suggesting that B. glabrata is a suitable test species for freshwater monitoring. Holding procedures and protocols implemented for toxicity and developmental bioassays showed to be in compliance with international standards for intra-laboratory precision. Thereby, we are proposing this system for application in ecotoxicological studies.Resumo IPEN-doc 16429 Efeitos citotóxicos e genotóxico de efluentes do rio Tietê, avaliados em celulas de ovário de hamster chines (CHO-K1), antes e após a passagem pela estação de tratamento de esgotos de Suzano, SP, Brasil2008 - SUZUKI, M.F.; TALLARICO, L.F.; BORRELY, S.I.; GRAZEFFE, V.S.; OHLWEILLER, F.; HAMADA, N.; PEREIRA, C.A.B.; NAKANO, E.; OKAZAKI, K.Resumo IPEN-doc 16433 Emprego de ensaios de toxicidade visando a avaliação da eficiência da ETE Suzano2008 - HAMADA, N.; TALLARICO, L.F.; SUZUKI, M.F.; GRAZEFFE, V.S.; PIRES, O.F.; OKAZAKI, K.; KAWANO, T.; PEREIRA, C.A.B.; NAKANO, E.; SPOSITO, R.D.; BORRELY, S.I.Resumo IPEN-doc 11572 Application of biological assays in the evaluation of water quality in Rio Tiete2006 - TALLARICO, L.F.; SUZUKI, M.F.; BORRELY, S.I.; GRAZEFFE, V.S.; OHLWEILER, F.P.; OKAZAKI, K.; OIKAWA, H.; BARROS, G.A.; HAMADA, N.; KAWANO, T.; PEREIRA, C.A.B.; NAKANO, E.Resumo IPEN-doc 16430 Assessment of Tietê river water using the mollusk biocomphalaria glabrata (Say, 1818)2008 - TALLARICO, L.F.; GRAZEFFE, V.S.; OHLWEILER, F.P.; OKAZAKI, K.; KAWANO, T.; PEREIRA, C.A. de B.; NAKANO, E.