MAYELLE MARIA PAZ LIMA

MAYELLE MARIA PAZ LIMA

Página do item completo

Resultados de Busca

Agora exibindo 1 - 5 de 5

High-throughput production of malignant melanoma spheroids (sk-mel-37) using simple plate treatment and automated fluorescence microscopy analysis
2024 - PRUDENTE, S.R.; SANTOS, E.C. dos; SILVA, G.D. da; LIMA, M.M.P.; FALCAO, P.L.; SILVA, T.M. da; VIEIRA, D.P.
The role of Prolactin (PRL) in activating the signaling pathways of the Jak STAT complex as a potential candidate in radioresistant MDAMB-231 cells in 3D cultures
2024 - FALCAO, P.L.; VIEIRA, D.P.; SILVA, G.D. da; NUNES, A.P.; MENEZES, F.; PRUDENTE, S.R.; SILVA, T.M. da; SANTOS, E.C. dos; PAZ, M.M.; SOARES, C.R.J.
Development of spheroids in co-culture of prostate tumor with human fibroblasts, using the hanging-drop technique with plate inversion for analysis of gamma irradiation by 60Co
2024 - SILVA, T.M.; RODRIGUES, A.A.; SILVA, G.D.; SANTOS, E.C.; LIMA, P.F.; PRUDENTE, S.R.; PAZ, M.M.; VIEIRA, D.P.
Development of human fibroblast spheroids with hanging-drop inverted plates
2023 - RODRIGUES, ALEX A.; SAMPAIO, MARLOS C.; SANTOS, ESTHER C. dos; PRUDENTE, SULEYNA R.; LIMA, MAYELLE M.P.; SILVA, GIOVANA D. da; MATHOR, MONICA B.; VIEIRA, DANIEL P.
Introduction and objective: 2D cultures have limitations in cell growth. 3D cultures, on the other hand, have become a valuable and powerful tool for biomedical research in recent decades. Due to their resemblance to living systems and cellular interactions, this type of culture can be developed using various methodologies, including nanoparticles, hydrogels, and layers of agarose, among others. Considering the need for testing and validating new molecules and effective therapies for treating various diseases, the objective of this study is to standardize a 3D human fibroblast culture model. Methodology: HF002-J, human fibroblast cells, were cultured at 37ºC in a humid atmosphere containing 5% CO2, maintained in RPMI 1640 medium supplemented with 10% fetal bovine serum and 1% antibiotics. When reaching 60- 70% confluence, the cells were detached using a 0.05% trypsin solution. Spheroids were prepared using the hangingdrop technique adapted from [1] 440 µL of medium containing cell variations ranging from 2 × 103 to 6 × 104 cells per well of a 96-well plate were deposited, generating a positive meniscus. The plate was inverted and incubated as described. Results and discussion: The present study aimed to evaluate the development of cellular spheroids after 4 days of culture using different cell preparations. Our results demonstrated that the preparations used produced compact spheroids, characterized by homogeneous sizes in the range of 500 to 1000 μm. When analysing the images obtained by wide-field fluorescence microscopy, we observed that the proportions of unviable cells labelled with fluorophores varied significantly according to the initial number of cells used in the preparations. Notably, increasing the initial number of cells resulted in a proportional increase in the number of non-viable cells present in the formed spheroids. These results suggest that the initial cell density can affect the development and viability of the formed spheroids. It is possible that too high cell density led to greater competition for nutrients and space, resulting in greater cell mortality and less viable spheroids. Conclusions: Based on the results obtained, it was possible to develop an initial prototype of spheroids from human fibroblast cells that can resemble tissues in vivo due to their cellular interactions, thus providing a new tool for the study of drugs and treatments.
Desenvolvimento de esferóides de melanoma humano como modelo alternativo ao uso de animais de laboratório
2023 - RODRIGUES, ALEX A.; SAMPAIO, MARLOS C.; SILVA, GIOVANA D. da; SANTOS, ESTHER C. dos; PRUDENTE, SULEYNA R.; LIMA, MAYELLE M.P.; VIEIRA, DANIEL P.