DALTON GIOVANNI NOGUEIRA DA SILVA
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Artigo IPEN-doc 26475 Multielemental analyses of Lonomia obliqua (Lepidoptera, Saturniidae) caterpillar using EDXRF and INAA techniques2019 - ZAMBONI, C.B.; GIOVANNI, D.N.S.; SALVADOR, V.L.R.; SATO, I.M.; MENDONĆA, R.Z.; WEN, F.H.; SIMONS, M.S.In this study, multielemental analysis of Lonomia obliqua (Lepidoptera, Saturniidae) caterpillar was performed using energy dispersive Xāray fluorescence and instrumental neutron activation analysis techniques. This caterpillar is poisonous and has the ability to cause fatal hemorrhagic effects in humans after contact. The need of this study is related to morphological changes (mainly size and color) observed in some caterpillars used for preparation of antilonomic serum (antivenom). The samples were classified as healthy (caterpillars of control) and unhealthy (caterpillars visibly modified). The XRF measurements were performed in an energy dispersive Xāray fluorescence spectrometer and the instrumental neutron activation analysis using the IEAR1 nuclear reactor at IPEN. The results show significant differences for several elements (mainly, P, Cl, Ca, Ti, Mn, Fe, Ni, and Zn) in unhealthy caterpillars that can affect the development of this species as well as the quality and yield of the antivenom. Furthermore, its elemental characterization contributes for the understanding the potential pharmacological (procoagulant and antithrombotic) in the prevention of lifeāthreatening blood clots.