PATRICIA ALVES DO NASCIMENTO
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Resumo IPEN-doc 07622 Avaliacao do efeito biologico da radiacao beta do sup(90)Sr em celulas sanguineas humanas pela tecnica de eletroforese de microgel (teste do cometa)2001 - OLIVEIRA, E.M.; SUZUKI, M.F.; SILVA, M.A.; NASCIMENTO, P.A.; OKAZAKI, K.Resumo IPEN-doc 06981 Avaliacao do dano radioinduzido e capacidade de reparo do DNA em pacientes com cancer de mama por meio da tecnica do cometa ('single cell gel electrophoresis')2000 - NASCIMENTO, P.A.; SILVA, M.A.; SUZUKI, M.F.; OLIVEIRA, E.M.; OKAZAKI, K.Resumo IPEN-doc 06975 Avaliação citogenética de linfócitos de pacientes com metástase óssea após a administração de EDTMP [Sm-153] para tratamento paliativo da dor2000 - SILVA, M.A.; NASCIMENTO, P.A.; SUZUKI, M.F.; OLIVEIRA, E.M.; ROGERO, J.R.; GUIMARAES, M.I.C.C.; BUCHPIGUEL, C.A.; OKAZAKI, K.Durante os últimos anos houve um aumento no interesse sobre a utilizaçõo de agentes radioterópicos como tratamento paliativo da dor óssea causada por metástases ósseos disseminadas. Objetivo: O objetivo deste trabalho é avaliar os efeitos do EDTMP [Sm-153] em linfócitos sanguíneos de pacientes com câncer de próstata. Método: Foram obtidos amostras sanguíneos de 12 pacientes com adenocorcinona de próstata, sendo 6 pacientes sem tratamento radioterópico (52 o 82 anos) e de 6 pacientes com tratamento prévio de radioterapia (60 a 88 anos). As amostras sanguíneos (5 mL) foram coletadas antes e uma hora após o administração endovenoso de EDTMP [Sm-153] (37 MBq/kg). As células foram processadas poro o análise de aberrações cromossômicas estruturais e numéricas. Resultados: Os principais tipos de aberrações cromossômicas estruturais observados foram os fragmentos acêntricos, quebras, gaps, dicêntricos , anéis e double minute. O número de aberrações/ célula na amostra sanguíneo uma hora após o administração de EDTMP [Sm-153] foi cerco de 2,13 vezes mais elevado em pacientes sem tratamento radioterápico ou quimioterápico, apresentando diferença estatisticamente significante em relação aos dados basais. Entretanto, nos pacientes submetidos o prévio tratamento radioterápico houve uma pequena redução no número de aberrações/célula, não sendo estatisticamente significante (p<0,05). Conclusão: O EDTMP [Sm-153] tem sido apontado como um radiofármaco terapêutico promissor no tratamento de pacientes com dor óssea resultante de metástases ósseas pois possui urna meio vida físico de 46,8 horas e é emissor de partículas ?. O estudo demonstrou que a frequência de aberrações/célula nas amostras submetidas ao EDTMP [Sm-153] foi mais elevada que nas amostras analisadas antes do administração (basal) do radionuclídeo nos pacientes sem tratamento radioterápico anterior.Resumo IPEN-doc 06979 Efeito citogenetico da radiacao beta do sup(90)Sr em celulas sanguineas humanas2000 - OLIVEIRA, E.M.; SILVA, M.A.; NASCIMENTO, P.A.; SUZUKI, M.F.; MURAKAMI, D.; OKAZAKI, K.Resumo IPEN-doc 06980 Avaliacao de dano radioinduzido por sup(153)Sm-EDTMP em linfocitos perifericos humanos2000 - SUZUKI, M.F.; MURAKAMI, D.; SILVA, M.A.; NASCIMENTO, P.A.; OLIVEIRA, E.M.; GUIMARAES, M.I.C.C.; OKAZAKI, K.Dissertação IPEN-doc 06883 Artigo IPEN-doc 06655 Evaluation of radioinduced damage and repair capacity in blood lymphocytes of breast cancer patients2001 - NASCIMENTO, P.A.; SILVA, M.A.; OLIVEIRA, E.M.; SUZUKI, M.F.; OKAZAKI, K.Genetic damage caused by ionizing radiation and repair capacity of blood lymphocytes from 3 breast cancer patients and 3 healthy donors were investigated using the comet assay. The comets were analyzed by two parameters: comet tail length and visual classification. Blood samples from the donors were irradiated in vitro with a 60Co source at a dose rate of 0.722 Gy/min, with a dose range of 0.2 to 4.0 Gy and analyzed immediately after the procedure and 3 and 24 h later. The basal level of damage and the radioinduced damage were higher in lymphocytes from breast cancer patients than in lymphocytes from healthy donors. The radioinduced damage showed that the two groups had a similar response when analyzed immediately after the irradiations. Therefore, while the healthy donors presented a considerable reduction of damage after 3 h, the patients had a higher residual damage even 24 h after exposure. The repair capacity of blood lymphocytes from the patients was slower than that of lymphocytes from healthy donors. The possible influence of age, disease stage and mutations in the BRCA1 and BRCA2 genes are discussed. Both parameters adopted proved to be sensitive and reproducible: the dose-response curves for DNA migration can be used not only for the analysis of cellular response but also for monitoring therapeutic interventions. Lymphocytes from the breast cancer patients presented an initial radiosensitivity similar to that of healthy subjects but a deficient repair mechanism made them more vulnerable to the genotoxic action of ionizing radiation. However, since lymphocytes from only 3 patients and 3 normal subjects were analyzed in the present paper, additional donors will be necessary for a more accurate evaluation.Artigo IPEN-doc 07134 Evaluation of the effect of 90Sr β-radiation on human blood cells by chromosome aberration and single cell gel electrophoresis (comet assay) analysis2001 - OLIVEIRA, E.M.; SUZUKI, M.F.; NASCIMENTO, P.A.; SILVA, M.A.; OKAZAKI, K.Among various environmental genotoxins, ionizing radiation has received special attention because of its mutagenic, carcinogenic and teratogenic potential. In this context and considering the scarcity of literature data, the objective of the present study was to evaluate the effect of 90Sr β-radiation on human cells. Blood cells from five healthy donors were irradiated in vitro with doses of 0.2-5.0 Gy from a 90Sr source (0.2 Gy/min) and processed for chromosome aberration analysis and for comet assay. The cytogenetic results showed that the most frequently found aberration types were acentric fragments, double minutes and dicentrics. The α and β coefficients of the linear-quadratic model, that best fitted the data obtained, showed that 90Sr β-radiation was less efficient in inducing chromosome aberrations than other types of low linear energy transfer (LET) radiation such as 3H β-particles, 60Co γ-rays, 137Cs and 192Ir and X-rays. Apparently, 90Sr β-radiation in the dose range investigated had no effect on the modal chromosome number of irradiated cells or on cell cycle kinetics. Concerning the comet assay, there was an increase in DNA migration as a function of radiation dose as evaluated by an image analysis system (tail moment) or by visual classification (DNA damage). The dose-response relation adequately fitted the non-linear regression model. In contrast to the cytogenetic data, 90Sr β-radiation induced more DNA damage than 60Co γ-radiation when the material was analyzed immediately after exposures. A possible influence of selective death of cells damaged by radiation was suggested.Artigo IPEN-doc 09856 High-level synthesis of recombinant murine endostatin in chinese hamster ovary cells2004 - CHURA-CHAMBI, R.M.; TORNIERI, P.H.; SPENCER, P.J.; NASCIMENTO, P.A.; MATHOR, M.B.; MORGANTI, L.