PATRICIA ALVES DO NASCIMENTO

PATRICIA ALVES DO NASCIMENTO

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Avaliacao da capacidade de reparo do dano radioinduzido em leucocitos sanguineos de pacientes com cancer de mama pela tecnica de eletroforese alcalina ('Comet Assay')
1998 - NASCIMENTO, P.A.; SILVA, M.A.; OLIVEIRA, E.M.; SUZUKI, M.F.; OKAZAKI, K.
Repair capacity of radioinduced DNA damage in breast cancer patients
1998 - NASCIMENTO, P.A.; SILVA, M.A.; OLIVEIRA, E.M.; SUZUKI, M.F.; OKAZAKI, K.
Capacidade de reparo do dano radioinduzido no DNA em pacientes com cancer de mama
1998 - NASCIMENTO, P.A.; SILVA, M.A.; OLIVEIRA, E.M.; SUZUKI, M.F.; OKAZAKI, K.
Estudo da radiolesao no DNA e proficiencia de reparo em linfocitos de individuos sadios
1997 - NASCIMENTO, P.A.; SUZUKI, M.F.; OKAZAKI, K.
Avaliacao do efeito biologico da radiacao beta do sup(90)Sr em celulas sanguineas humanas pela tecnica de eletroforese de microgel (teste do cometa)
2001 - OLIVEIRA, E.M.; SUZUKI, M.F.; SILVA, M.A.; NASCIMENTO, P.A.; OKAZAKI, K.
Avaliacao do dano radioinduzido e capacidade de reparo do DNA em pacientes com cancer de mama por meio da tecnica do cometa ('single cell gel electrophoresis')
2000 - NASCIMENTO, P.A.; SILVA, M.A.; SUZUKI, M.F.; OLIVEIRA, E.M.; OKAZAKI, K.
Efeito citogenetico da radiacao beta do sup(90)Sr em celulas sanguineas humanas
2000 - OLIVEIRA, E.M.; SILVA, M.A.; NASCIMENTO, P.A.; SUZUKI, M.F.; MURAKAMI, D.; OKAZAKI, K.
Avaliacao do dano radioinduzido no DNA e reparo em linfocitos humanos pelo metodo do Cometa ('single cell gel electrophoresis')
1997 - NASCIMENTO, P.A.; SUZUKI, M.F.; OKAZAKI, K.
Avaliacao do dano radioinduzido e capacidade de reparo do dna em pacientes com cancer de mama por meio da tecnica do cometa [ ' single cell gel electrophoresis ' ]
2000 - NASCIMENTO, PATRICIA A. do
Evaluation of radioinduced damage and repair capacity in blood lymphocytes of breast cancer patients
2001 - NASCIMENTO, P.A.; SILVA, M.A.; OLIVEIRA, E.M.; SUZUKI, M.F.; OKAZAKI, K.
Genetic damage caused by ionizing radiation and repair capacity of blood lymphocytes from 3 breast cancer patients and 3 healthy donors were investigated using the comet assay. The comets were analyzed by two parameters: comet tail length and visual classification. Blood samples from the donors were irradiated in vitro with a 60Co source at a dose rate of 0.722 Gy/min, with a dose range of 0.2 to 4.0 Gy and analyzed immediately after the procedure and 3 and 24 h later. The basal level of damage and the radioinduced damage were higher in lymphocytes from breast cancer patients than in lymphocytes from healthy donors. The radioinduced damage showed that the two groups had a similar response when analyzed immediately after the irradiations. Therefore, while the healthy donors presented a considerable reduction of damage after 3 h, the patients had a higher residual damage even 24 h after exposure. The repair capacity of blood lymphocytes from the patients was slower than that of lymphocytes from healthy donors. The possible influence of age, disease stage and mutations in the BRCA1 and BRCA2 genes are discussed. Both parameters adopted proved to be sensitive and reproducible: the dose-response curves for DNA migration can be used not only for the analysis of cellular response but also for monitoring therapeutic interventions. Lymphocytes from the breast cancer patients presented an initial radiosensitivity similar to that of healthy subjects but a deficient repair mechanism made them more vulnerable to the genotoxic action of ionizing radiation. However, since lymphocytes from only 3 patients and 3 normal subjects were analyzed in the present paper, additional donors will be necessary for a more accurate evaluation.