JORGE GABRIEL DOS SANTOS BATISTA

JORGE GABRIEL DOS SANTOS BATISTA

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Advances in silver nanoparticles
2024 - RODRIGUES, ADRIANA S.; BATISTA, JORGE G.S.; RODRIGUES, MURILO A.V.; THIPE, VELAPHI C.; MINARINI, LUCIENE A.R.; LOPES, PATRICIA S.; LUGAO, ADEMAR B.
Nanoparticles play a crucial role in the field of nanotechnology, offering different properties due to their surface area attributed to their small size. Among them, silver nanoparticles (AgNPs) have attracted significant attention due to their antimicrobial properties, with applications that date back from ancient medicinal practices to contemporary commercial products containing ions or silver nanoparticles. AgNPs possess broad-spectrum biocidal potential against bacteria, fungi, viruses, and Mycobacterium, in addition to exhibiting synergistic effects when combined with certain antibiotics. The mechanisms underlying its antimicrobial action include the generation of oxygen-reactive species, damage to DNA, rupture of bacterial cell membranes and inhibition of protein synthesis. Recent studies have highlighted the effectiveness of AgNPs against various clinically relevant bacterial strains through their potential to combat antibiotic-resistant pathogens. This review investigates the proteomic mechanisms by which AgNPs exert their antimicrobial effects, with a special focus on their activity against planktonic bacteria and in biofilms. Furthermore, it discusses the biomedical applications of AgNPs and their potential non-preparation of antibiotic formulations, also addressing the issue of resistance to antibiotics.
Fungal nanobionics
2023 - THIPE, VELAPHI C.; BATISTA, JORGE G.S.; LEBRE, DANIEL T.; LUGAO, ADEMAR B.; KATTI, KATTESH V.
Fungi have proven to be one of the most beneficial microorganisms that have contributed and continue to play a pivotal role in human civilization. The nexus between mycology and nanotechnology provides a cornucopia of metabolites that can be utilized in nanotechnology to usher in a separate branch of study—"Myconanobiotechnology.” This is achieved by fungal nanobionics, which centers fungi as nanofactories for the large-scale industrial production of nanoparticles for the development of new next-generation products with industrial, agricultural, medicinal, and consumer application prospects in a wide range of sectors that stem from pharmaceuticals, agriculture, and energy, thus envisaging a completely different paradigm in relation to revolutionizing technologies. In this chapter, we cover a plethora of the biogenic-mediated synthesis of nanoparticles intracellularly and extracellularly with an in-depth understanding of the principles that govern fungal nanobionics. Furthermore, we provide an in-depth knowledge of the most recent advances in mycoinnovations that utilize fungal nanobionics in the ubiquitous role they play in a myriad of applications stemming from disinfectants, food packaging material additives, biosensors, medicine, cosmetics, textiles, electronics, composites, agricultural products (effective antimicrobial agents in nanofertilizers and nanopesticides), and most recently in energy through renewable, sustainable biofuel production.
Copper-Based Nanomaterials for Biologically Relevant Compounds
2024 - BATISTA, JORGE G. dos S.; RODRIGUES, MURILO A.V.; FREITAS, LUCAS F. de; FONSECA, ANA C.M.; RODRIGUES, ADRIANA de S.; CRUZ, CASSIA P.C. da; THIPE, VELAPHI C.; LUGAO, ADEMAR B.
Copper-based nanomaterials have emerged as a new platform in various scientific domains due to their unique physicochemical, biological, and compatibility properties. This review explores the multifaceted applications of copper nanomaterials in the areas of biosensing, drug delivery, cancer therapy, and environmental remediation. The innovative role of copper nanomaterials in biosensors and their exceptional sensitivity and specificity in detecting biomolecules are discussed. Moreover, the potential applications of copper-based nanomaterials in drug delivery systems, highlighting the controlled and targeted release of therapeutic agents’ approaches are explored. In the domain of cancer theragnostic, copper nanomaterials exhibit promising results thus offering practical tools for early diagnosis and advanced treatment modalities. Furthermore, the antimicrobial properties of copper nanoparticles are explored, emphasizing their importance in combating microbial threats and promoting a new era in antimicrobial research. Copper-based nanomaterials could have a significant role in environmental and bioremediation applications. The strong catalytic activity of copper nanoparticles makes them ideal candidates for environmental remediation. In summary, this comprehensive review consolidates the latest advances in copper nanomaterials research, providing valuable information for scientists and researchers involved in diverse fields to promote the development of new applications and technologies.
Silver nanoparticles reduced by tannic acid and sodium citrate
2023 - RODRIGUES, ADRIANA S.; BATISTA, JORGE G.S.; MINARINI, LUCIENE A.R.; LOPES, PATRICIA; LUGAO, ADEMAR B.
Introduction and objective: Green nanotechnology aims to reduce hazardous chemical waste in the environment through sustainable development. Phytochemicals are proposed to minimize environmental impacts and produce safe biological applications. Silver nanoparticles (AgNPs) have effective antimicrobial properties against Gram-positive and Gram-negative bacterias, fungi and viruses, being promising to reduce the microbial load. Antimicrobial systems based on AgNPs shows promise in combating bacteria. Methodology: Confirmation of the formation of silver nanoparticles was evaluated by UV-Vis spectrophotometry. The hydrodynamic size and polydispersion index were evaluated by dynamic light scattering. The zeta potential was used to assess stability through surface charge. The obtained morphology and average size were evaluated by transmission electronic microscopy. The cytotoxicity assay was performed to assess cellular viability of silver nanoparticles in HUVEC cells. The antimicrobial activity was analyzed by minimum inhibitory concentration through microdilution in broth and later the inoculum was performed in plates. Results and discussion: Characterization by spectrophotometry of AT_AgNPs and CT_AgNPs showed absorption bands at 430 nm. Hydrodynamic size analyzes revealed diameters of 57.87-97.45 nm, with polydispersion indices (PdI) ranging between 0.289 and 0.392. The zeta potential was determined between - 4.41 and -10.3 mV. Transmission electron microscopy (TEM) images revealed spherical morphology with sizes between 20-50 nm. AgNPs have been tested as a treatment against hospital microorganisms with risk classification level 2, including Gram-negative (Acinetobacter baumannii, Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa) and Gram-positive (Staphylococcus aureus) bacteria. The Minimum Inhibitory Concentration (MIC) was determined to evaluate the lowest concentration that inhibits the growth of microorganisms. The MIC values obtained with the AT_AgNPs were: 4.18 ug/mL (AB14 and EC23), 8.36 ug/mL (EC26 and KP43), 16.72 ug/mL (PA17), 66.9 ug/mL (PA3) and 133.8 ug/mL (SA). For CT_AgNPs, they were: 51.8 ug/mL (AB14, EC26 and PA17), without inhibition in EC23, KP43, PA3 and SA strains. Synergism was evaluated by mixing suspensions of the two nanoparticles (ATCT_AgNPs), resulting in MIC values of 1.48 ug/mL (PA3 and SA), 2.96 ug/mL (EC23, KP43 and PA17) and 5.93 ug/mL (AB14 and EC26). Conclusions: The results suggest that AT_AgNPs and the synergistic combination with CT_AgNPs have potential as effective antimicrobial agents against nosocomial bacteria. These AgNPs may represent a promising alternative for the development of new therapeutic strategies to combat bacterial infections. However, further studies are needed to investigate its activity in other contexts and its safety in clinical use.
Fabrication of green nanomaterials
2023 - THIPE, VELAPHI C.; FREITAS, LUCAS F.; LIMA, CAROLINE S.A.; BATISTA, JORGE G.S.; FERREIRA, ARYEL H.; OLIVEIRA, JUSTINE P.R. de; BALOGH, TATIANA S.; KADLUBOWSKI, SLAWOMIR; LUGAO, ADEMAR B.; KATTI, KATTESH V.
The purpose of this chapter is to discuss the production of biocompatible green nanomaterials for biomedical applications using green nanotechnology. To enhance drug loading and delivery, these nanomaterials are engineered with immunomodulatory ligands such as phytochemicals (Epigallocatechin gallate, Mangiferin, Resveratrol), proteins (albumin and papain), crosslinked hydrogels, and nanogels. The nanomaterials described herein are synthesized via redox potential of electron-dense phytochemicals that reduce metallic precursors to their stable corresponding nanoparticles and via water radiolysis with ionizing radiation as a green approach (due to the absence of any reducing agent) for use as radiosensitizers (albumin and papain nanoparticles) in nuclear medicine – theranostics applications. The phytochemicals facilitate the delivery of nanoparticles through receptor mediated endocytosis, while the proteins such as papain, due to their proteolytic action enhances the permeation of nanoparticles into tumor tissue, and albumin increase the pharmacokinetic efficiency of these nanoparticles. The nanoparticles developed have shown effectiveness against a variety of human cancers while posing no toxicity to normal tissue. Additionally, a pilot human clinical combing Ayurvedic medicine with green nanomedicine is given as a novel approach for treating breast cancer and other related illnesses. Finally, the importance of ecotoxicology for nanomaterials is discussed in order to provide safety data in relevant multiple species (fish, daphnia, algae, rodents, etc.) with paratope/epitope distributions for evaluating tissue cross-reactivity profiles in human tissues and to provide critical information on in vivo toxicity in order to predict the possible adverse effects of nanomaterials on human and environmental health as an effort to establish regulatory limits and ISO standards for nanomaterials.
The role of probiotics in maintaining immune homeostasis
2022 - THIPE, VELAPHI C.; MENTOR, SHIREEN; LIMA, CAROLINE S.A.; FREITAS, LUCAS F.; FONSECA, ANA C.M.; NOGUEIRA, KAMILA M.; RODRIGUES, ADRIANA S.; BATISTA, JORGE G.S.; FERREIRA, ARYEL H.; LUGAO, ADEMAR B.
The immune system is a complex architecture of a collective and coordinated network regulated by various pathways to thermodynamically maintain immune homeostasis. The gut microbiota plays a pivotal role that offers significant stimuli (i.e., gut-brain, gut-lung, and gut-liver axis) for both innate and adaptive immunity, mediating immune and metabolic homeostasis. An intricate correlation between changes in the gut microbiota (dysbiosis) and common diseases/disorders have been attributed to the invasion of pathogens, constant use of antibiotics, and hypercytokinemia—a hallmark of immune homeostasis imbalance. These factors contribute to the severity of inflammatory diseases such as cardiovascular diseases, neurological disorders, and of late the coronavirus disease, Covid-19. Probiotics (Lactobacillus spp. and Bifidobacterium spp.) have been considered as alternative and/or adjuvant therapeutic in restoring the balance of gut microbiota for maintaining immune homeostasis and integrity. The probiotics catalyze dietary fibers and proteins to generate short-chain fatty acids and tryptophan to promote antiinflammatory cytokines, reduce epithelium permeability, reinforcing immunity in the gut mucosa, and regulating the systemic immune response. Herein, we review our overarching understanding of current applications of probiotics in amelioration of gut microbiome, and the improvement of gut barrier function and maintaining immune homeostasis. We also highlight clinical trials on probiotics with reported results for the treatment of inflammatory diseases. Additionally, the looming global Covid-19 pandemic makes it prudent to highlight the role of probiotics in both the innate and adaptive human immune responses, especially amid the Covid-19 vaccination paradigm.
Cu and Cu-based nanomaterials as nanofungicides
2022 - THIPE, VELAPHI C.; FREITAS, LUCAS F.; LIMA, CAROLINE S.A. de; NOGUEIRA, KAMILA M.; BATISTA, JORGE G.S.; FERREIRA, ARYEL H.; LUGAO, ADEMAR B.
Green nanotechnology through the production of copper and copper oxide nanoparticles (CuNPs and CuONPs, respectively) as nanofungicides brings forth the opportunity attributed to their antimicrobial properties in addition to Cu being an essential metal micronutrient that functions as a cofactor for many enzymatic activities in plants. Herein, we explore the use of CuNPs and CuONPs as nanofungicides against toxigenic fungi and their mechanism of action. We also highlight the green nanoagriculture and the ecotoxicology and safety of CuNPs and CuONPs as nanofungicides to significantly aid as agricultural breakthroughs because such approaches will provide realistic sustainable nano Cu-enabled products deemed safe for agricultural practices.
Copper nanomaterials for eliminating the risk of mycotoxins
2022 - THIPE, VELAPHI C.; BATISTA, JORGE G.S.; LUGAO, ADEMAR B.
Mycotoxins continue to pose significant challenges in agriculture and health sector. The most feasible strategy to eliminate their manifestation and risk is the use of fungicides against mycotoxigenic fungal contamination as an indirect approach. However, conventional fungicides have not been effectively attributed to acquired fungicide resistance and high toxicity on plants, animals, and human health. Copper (Cu) has been recognized as an essential micronutrient metal/mineral required by plants that participate in various physiological processes (chlorophyll and photosynthesis) and as a cofactor for enzymatic reactions including activation of many metalloproteins and those involved in lignin synthesis. Herein, we review Cu nanomaterials for the risk of mycotoxin also; we explore their nanofungicidal activity and the comprehensive understanding of the multitude of the tripartite interactions of Cu nanomaterials with the ecosystem (plants, soil, and animals/humans), and green nanotechnological approach in limiting their toxicity profile. Lately, we discuss some of the future recommendations for Cu nanofungicides toward eliminating the risk of mycotoxicology.
Silver nanoparticles applications and ecotoxicology for controlling mycotoxins
2021 - THIPE, VELAPHI C.; LIMA, CAROLINE S.A.; NOGUEIRA, KAMILA M.; BATISTA, JORGE G.S.; FERREIRA, ARYEL H.; KATTI, KATTESH V.; LUGAO, ADEMAR B.
The state of the art of theranostic nanomaterials for lung, breast, and prostate cancers
2021 - FREITAS, LUCAS F.; FERREIRA, ARYEL H.; THIPE, VELAPHI C.; VARCA, GUSTAVO H.C.; LIMA, CAROLINE S.A.; BATISTA, JORGE G.S.; RIELLO, FABIANE N.; NOGUEIRA, KAMILA; CRUZ, CASSIA P.C.; MENDES, GIOVANNA O.A.; RODRIGUES, ADRIANA S.; SOUSA, THAYNA S.; ALVES, VICTORIA M.; LUGAO, ADEMAR B.
The synthesis and engineering of nanomaterials offer more robust systems for the treatment of cancer, with technologies that combine therapy with imaging diagnostic tools in the so‐called nanotheranostics. Among the most studied systems, there are quantum dots, liposomes, polymeric nanoparticles, inorganic nanoparticles, magnetic nanoparticles, dendrimers, and gold nanoparticles. Most of the advantages of nanomaterials over the classic anticancer therapies come from their optimal size, which prevents the elimination by the kidneys and enhances their permeation in the tumor due to the abnormal blood vessels present in cancer tissues. Furthermore, the drug delivery and the contrast efficiency for imaging are enhanced, especially due to the increased surface area and the selective accumulation in the desired tissues. This property leads to the reduced drug dose necessary to exert the desired effect and for a longer action within the tumor. Finally, they are made so that there is no degradation into toxic byproducts and have a lower immune response triggering. In this article, we intend to review and discuss the state‐of‐the‐art regarding the use of nanomaterials as therapeutic and diagnostic tools for lung, breast, and prostate cancer, as they are among the most prevalent worldwide.