FERNANDA DOS SANTOS ARTHUSO
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Artigo IPEN-doc 24236 Determination of recombinant Interferon-α2 in E. coli periplasmic extracts by reversed-phase high-performance liquid chromatography2018 - DIAS, PAULO V.S.; ARTHUSO, FERNANDA S.; OLIVEIRA, JOAO E.; SUZUKI, MIRIAM F.; SOUSA, JOSE M.; RIBELA, MARIA T.C.P.; BARTOLINI, PAOLO; SOARES, CARLOS R.J.Reversed-phase high-performance liquid chromatography (RP-HPLC) has been used to analyze Interferon α-2 (IFN-α2) as a pure protein or as a pharmaceutical preparation: a method for analyzing periplasmic IFN-α2 directly in osmotic shock extract has, however, never been reported. This work describes an RP-HPLC methodology for the qualitative and quantitative analysis of human IFN-α2a and IFN-α2b directly in bacterial periplasmic extracts or in purified preparations. The analytical method has been set up and validated for accuracy, precision, linearity, sensitivity and specificity. A recovery test indicated an average bias of ∼1%, intra-day and inter-day quantitative determinations presented relative standard deviations always ≤ 5%, while the working sensitivity was of ∼0.3 μg of IFN-α2 (RSD =5%). The method proved to be suitable for detecting and quantifying also glycosylated and oxidized forms and N-methionylated IFN-α2 molecules, it was, however, not able to distinguish between IFN-α2a and IFN-α2b. This rapid methodology allows the application of RP-HPLC as a powerful tool to monitor the production yield and quality of IFN-α2 in osmotic shock fluids, right after, or even during the fermentation process.Resumo IPEN-doc 17621 A practical and fast adaptation of CHO cells expressing human prolactin to grow in suspension and its application to laboratory production2011 - ARTHUSO, F.S.; CAPONE, M.V.N.; SUZUKI, M.F.; SOUSA, J.M.; OLIVEIRA, J.E.; BARTOLINI, P.; SOARES, C.R.J.Resumo IPEN-doc 16598 Purification and characterization recombinant glycosylated human prolactin synthesized in cho cells2008 - OLIVEIRA, T.L.; HELLER, S.R.; OLIVEIRA, J.E.; ARTHUSO, F.S.; GOULART, H.R.; SOUSA, J.M.; SUZUKI, M.F.; BARTOLINI, P.; SOARES, C.R.S.Resumo IPEN-doc 15608 Physico-chemical and biological characterization of glycosylated human prolactin produced at IPEN2010 - GOULART, H.R.; ARTHUSO, F.S.; CAPONE, M.V.N.; OLIVEIRA, T.L.; OLIVEIRA, J.E.; SUZUKI, M.F.; SOUSA, J.M.; BARTOLINI, P.; SOARES, C.R.J.Resumo IPEN-doc 12449 The effect of cycloheximide on the glycosylation of recombinant human prolactin synthesized in cho cells2007 - HELLER, S.R.; OLIVEIRA, T.L.; ARTHUSO, F.S.; SOUSA, J.M.; BARTOLINI, P.; SOARES, C.R.Resumo IPEN-doc 12448 Bioactive and properly folded prolactin is obtained in E.coli in a pilot bioreactor at 37 graus C by constitutive expression based on the unrepressed ?Psub(L) promoter2007 - OLIVEIRA, T.L.; HELLER, S.R.; ARTHUSO, F.S.; SOUSA, J.M.; BARTOLINI, P.; SOARES, C.R.