MURILO ALISON VIGILATO

MURILO ALISON VIGILATO

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Comparing solution blow spinning and electrospinning methods to produce collagen and gelatin ultrathin fibers
2024 - RODRIGUES, MURILO A.V.; BERTOLO, MIRELLA R.V.; HORN, MARILIA M.; LUGAO, ADEMAR B.; MATTOSO, LUIZ H.C.; PLEPIS, ANA M. de G.
Ultrathin fibers have been used to design functional nanostructured materials for technological and biomedical applications. Combining the use of renewable and compatible sources with the emerging alternative SBS (solution blow spinning) technique opens new opportunities for material applications. In this review, we introduce the benefits of SBS over the classical electrospinning technique by following studies that use collagen or gelatin. SBS offers distinct advantages over electrospinning in the preparation of ultrathin fibers based on natural proteins, including the absence of high-voltage sources and the possibility of using fewer toxic solvents. Notably, there is also the prospect of using SBS directly in injured tissues, opening new strategies for in situ structure assembly SBS is a suitable approach to produce fibers at the nanoscale that can be tailored to distinct diameters by blending or simply adjusting experimental conditions. The focus on producing collagen or gelatin fibers contributes to designing highly biocompatible mats with potential for promoting cellular growth and implantation, even though their applications can be found also in food packaging, energy, and the environment. Therefore, a comprehensive analysis of the topic is essential to evaluate the current strategies regarding these materials and allow for their expanded production and advanced applications.
Desenvolvimento de um dispositivo controlador de ondas mecânicas usando plataforma Arduino para aplicações em regeneração tecidual
2024 - SASDELLI, GABRIEL C.A.; SANTOS, LARISSA S. dos; RODRIGUES, MURILO A.V.; LUGAO, ADEMAR B.
Síntese e caracterização de nanopartículas de ouro
2024 - ALMEIDA, BIANCA O.E.; RODRIGUES, MURILO A.V.; LUGAO, ADEMAR B.
Advances in silver nanoparticles
2024 - RODRIGUES, ADRIANA S.; BATISTA, JORGE G.S.; RODRIGUES, MURILO A.V.; THIPE, VELAPHI C.; MINARINI, LUCIENE A.R.; LOPES, PATRICIA S.; LUGAO, ADEMAR B.
Nanoparticles play a crucial role in the field of nanotechnology, offering different properties due to their surface area attributed to their small size. Among them, silver nanoparticles (AgNPs) have attracted significant attention due to their antimicrobial properties, with applications that date back from ancient medicinal practices to contemporary commercial products containing ions or silver nanoparticles. AgNPs possess broad-spectrum biocidal potential against bacteria, fungi, viruses, and Mycobacterium, in addition to exhibiting synergistic effects when combined with certain antibiotics. The mechanisms underlying its antimicrobial action include the generation of oxygen-reactive species, damage to DNA, rupture of bacterial cell membranes and inhibition of protein synthesis. Recent studies have highlighted the effectiveness of AgNPs against various clinically relevant bacterial strains through their potential to combat antibiotic-resistant pathogens. This review investigates the proteomic mechanisms by which AgNPs exert their antimicrobial effects, with a special focus on their activity against planktonic bacteria and in biofilms. Furthermore, it discusses the biomedical applications of AgNPs and their potential non-preparation of antibiotic formulations, also addressing the issue of resistance to antibiotics.
Copper-Based Nanomaterials for Biologically Relevant Compounds
2024 - BATISTA, JORGE G. dos S.; RODRIGUES, MURILO A.V.; FREITAS, LUCAS F. de; FONSECA, ANA C.M.; RODRIGUES, ADRIANA de S.; CRUZ, CASSIA P.C. da; THIPE, VELAPHI C.; LUGAO, ADEMAR B.
Copper-based nanomaterials have emerged as a new platform in various scientific domains due to their unique physicochemical, biological, and compatibility properties. This review explores the multifaceted applications of copper nanomaterials in the areas of biosensing, drug delivery, cancer therapy, and environmental remediation. The innovative role of copper nanomaterials in biosensors and their exceptional sensitivity and specificity in detecting biomolecules are discussed. Moreover, the potential applications of copper-based nanomaterials in drug delivery systems, highlighting the controlled and targeted release of therapeutic agents’ approaches are explored. In the domain of cancer theragnostic, copper nanomaterials exhibit promising results thus offering practical tools for early diagnosis and advanced treatment modalities. Furthermore, the antimicrobial properties of copper nanoparticles are explored, emphasizing their importance in combating microbial threats and promoting a new era in antimicrobial research. Copper-based nanomaterials could have a significant role in environmental and bioremediation applications. The strong catalytic activity of copper nanoparticles makes them ideal candidates for environmental remediation. In summary, this comprehensive review consolidates the latest advances in copper nanomaterials research, providing valuable information for scientists and researchers involved in diverse fields to promote the development of new applications and technologies.
Ultrathin collagen and gelatin fibers
2023 - RODRIGUES, MURILO A.V.; MARTINS, VIRGINIA da C.A.; LUGAO, ADEMAR B.; MATTOSO, LUIZ H.C.; PLEPIS, ANA M. de G.
Introduction and objective: The main constituent of extracellular matrix (ECM) of various tissues is collagen, a biodegradable and biocompatible protein with excellent regenerative properties. A promising way to produce scaffolds, artificial ECM, involves the production of nanometric or submicrometric fibers, dimension of the natural fibers found in the ECM of many tissues. Solution blow spinning (SB-Spinning) allows the production of fibers with high feed rates and in situ deposition. Here we produce ultrathin collagen and gelatin (polymer obtained from collagen denaturation) fibers by SB-Spinning using solvents that preserve the integrity of the polymers and evaluate the morphology and properties of these fibers [1,2]. Methodology: Collagen (10 wt%) and gelatin (10 and 15 wt%) solutions were prepared in 90 wt% acetic acid under stirring overnight at room temperature. A glass syringe was used to spin 25 cm (for gelatin) or 20 cm (for collagen) away from the collector using 30 psi (gelatin) or 10 psi (collagen) of pressure at 3.6 to 10.8 mL/h for gelatin and at 3 to 6 mL/h for collagen. The fibers were characterized by scanning electron microscopy, differential scanning calorimetry and polyacrylamide gel electrophoresis. Results and discussion: Gelatin and collagen submicrometric fibers were produced from 90% acetic acid solutions, a benign solvent that allows the solubilization of high amounts of these polymers and present low toxicity with low cost, what reveals it as a promising benign solvent for this application. Gelatin fibers presented average diameters between 740 ± 299 nm and 909 ± 326 nm for 15% solutions and between 175 ± 64 nm and 196 ± 113 nm for 10% solutions, indicating the direct effect of polymer concentration on the diameter of fibers. Collagen fibers presented average diameters between 542 ± 185 nm and 543 ± 242 nm, being thicker than the gelatin fibers obtained with the same polymer concentration, an indicative of the preservation of the natural structure of these protein, the triple helix secondary structure. The preservation of collagen triple helix after the spinning process was confirmed by differential scanning calorimetry and gel electrophoresis results. Conclusions: Aqueous acetic acid was considered a good solvent for the solubilization of these collagenous proteins, being adequate to the production of biomaterials due to its low toxicity and ability to preserve the natural structure of collagen. It was also possible to understand the effect of some production parameters on the diameter of these fibers, an important step in the development of new biomimetic biomaterials produced by solution blow spinning.
Production of polycaprolactone/curcumin membranes and ITS effectiveness in cells growth, pH sensitivity and release of curcumin
2023 - SANTOS, LARISSA S. dos; RODRIGUES, MURILO A.V.; FREITAS, LUCAS F. de; FERREIRA, ARYEL H.; LUGAO, ADEMAR B.
Polyviniyl alcohol mats crosslinked with citric acid for regenerative medicine applications
2023 - SASDELLI, GABRIEL C.A.; RODRIGUES, MURILO A.V.; LUGAO, ADEMAR B.
Introduction: Currently, biomaterials have stood out in the medical field, seeking innovative solutions for wound healing. Papain, an enzyme with remarkable healing properties, shows promise in this context. However, its activity is compromised when in contact with water and at high temperatures. To overcome this challenge, this project has considered the use of polyvinyl alcohol (PVA) membranes, a low-toxicity, costeffective and hydrophilic polymer, offering an effective and safe approach to promote wound healing. However, the solubility of this polymer restrains its use, thus the aim of this project is to crosslink PVA fibers directly during solution blow spinning procedure. Methodology: Mats were produced using a Solution Blow Spinning (SBS) device with a rotary collector. The SBS device was 40 cm away from the collector and the solution was supplied with a pump at 3mL/h through a syringe using air at 6 psi of pressure. Mats were prepared using a 14 wt% PVA (65% high molecular weight/35% low molecular weight) solution in water and compared with solutions containing different concentrations of citric acid. The mechanical and rheological characterizations were done by tensile strength and viscosity tests, while infrared spectroscopy and scanning electron microscopy were employed to analyze fibers structure and morphology. Results and discussion: The mats produced in this study were consistently white, irrespective of citric acid presence. With increasing citric acid concentration, the mats showed improved resistance, making them more durable for various applications. The addition of citric acid also reduced mats water solubility, enhancing their long-term stability in different environments, notably biological fluids as exudate. Infrared spectroscopy analysis confirmed the crosslinking, while scanning electron microscopy revealed mats were composed by ultrafine fibers. These improved properties obtained by crosslinking make the mats highly suitable for practical applications of wound dressing, mainly by the use of safe crosslinking agent. The PVA solutions presented a higher viscosity with increasing citric acid content, presenting viscosity values of 0.9 ± 0.03 Pa s and 1.19 ± 0.07 Pa s for 0 wt% and 10 wt% of citric acid (in reference to the PVA mass) at 21,5 s-1. These findings hold significant promise for expanding crosslinked PVA mats usage in various types of wounds. Conclusions: Based on our results, it is possible to assert that with the addition of citric acid, which promoted polymer crosslinking, we obtained PVA dressings with ideal properties for wound use. Since the dressings are in perfect condition to withstand wound moisture, we can now consider the inclusion of papain in the mats, that can be stored dry and hydrated just in the moment of use, an attempt to preserve the hydrolysis of these enzyme.
Antitumoral efficacy of gold nanoparticles with polyphenols in breast cancer and metastatic cells in vitro
2023 - SPADREZANO, ISADORA; RODRIGUES, MURILO A.V.; VIEIRA, DANIEL P.; LUGAO, ADEMAR B.
Introduction and objective: Gold nanoparticles (AuNPs) present beneficial properties in cancer diagnosis and therapy, as they can be coated and biofunctionalized with bioactive molecules through surface modification using relatively non‐toxic reagents, enabling the reduction of metal ions, stabilization, and selective detection of cellular receptors [1]. The reduction of gold by phytochemicals to form nanoparticles represents promising methods in Green Nanotechnology. This study aims to compare the binding capacity and evaluate the antiproliferative potential of gold nanoparticles coated with tannic acid (TA‐AuNPs) against human breast tumor cell lines. Cytotoxicity was determined using the MTS method. Methodology: The synthesis of TA‐AuNPs was established through the chemical reduction, and the purification method was based on centrifugation, where the centrifuged sample (P1) was resuspended after removing and storing the supernatant (S1), thus generating three samples, including the non‐centrifuged (NC). The confirmation of TA‐AuNPs formation was achieved by UV‐Visible absorption spectroscopy, and the size determination of TA‐AuNPs was carried out using dynamic light scattering and Transmission Electron Microscopy (TEM) techniques. The Zeta potential was used to determine the stability of TA‐AuNPs. Human breast adenocarcinoma cell lines (MCF‐7 and MDA‐MB‐231) were used, and the cytotoxicity determination was performed using the MTS assay. Results and discussion: The synthesis of TA‐AuNPs showed a change in color, indicating the formation of spherical AuNPs. UV‐Visible analysis was performed for preliminary characterization, where the absorption band at a wavelength of 529 nm, correlated with the localized surface plasmon resonance band, indicates the presence of approximately 20 nm AuNPs in all samples. To evaluate the size of TA‐AuNPs, the samples were analyzed using the dynamic light scattering method. The hydrodynamic diameters measured indicate that centrifugation induces greater aggregation of the AuNPs. However, based on the polydispersity values ranging from 0.087 ‐ 0.186, the AuNPs showed uniform sizes. The Zeta potential was an effective indicator used as a criterion to classify and quantify stability, with the samples exhibiting magnitudes above 40 mV, indicating high stability. TEM images confirmed the presence of TA‐AuNPs in the samples and provided information about their dimensions, around approximately 20 nm with spherical morphology. The cytotoxicity assessment for validating TA‐AuNPs demonstrated that the coating plays a crucial role in the degree of internalization, and a higher quantity of specific receptors resulted in a greater internalization of TA‐ AuNPs. It was observed that there was a higher percentage of cell viability in MCF‐7 cells compared to MDA‐MB‐ 231 cells. Conclusions: The synthesis was efficient in obtaining TA‐AuNPs. The TA‐AuNPs showed a narrow size distribution, predominantly spherical morphology, and low PDI, indicating significant potential for biomedical applications in the healthcare field. The results satisfactorily and efficiently demonstrated that TA‐AuNPs are effective against breast cancer cells (MCF‐7) and exhibit higher efficiency in targeting metastatic breast cancer cells (MDA‐MB‐ 231), highlighting their significant potential for various medical applications in the field of nanomedicine.
Produção de mantas de policaprolactona para liberação de curcumina
2023 - SANTOS, LARISSA S. dos; RODRIGUES, MURILO A.V.; LUGAO, ADEMAR B.