JUSTINE PAULA RAMOS DE OLIVEIRA
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Resumo IPEN-doc 29448 A biological study of gelatin-PVA based scaffold functionalized with albumin for biomedical purposes2022 - VARCA, J.O.; KLINGBEIL, F.; NOGUEIRA, K.M.; LIMA, C.S.; CRUZ, C.C. da; FREITAS, L.F.; VARCA, G.H.; MATHOR, M.B.; LUGAO, A.B.Biomaterials have been designed for tissue reconstruction, bone regeneration and cell culture, and functionalized with presence of proteins, nanoparticles, peptides and other components to improve the biocompatibility for instance. This work shows a biological study of gelatin-PVA based scaffold with controlled pore size and functionalized with albumin for biomedical purposes. The in vitro study comprises cytotoxicity, cell adhesion and proliferation assessment. In practical terms, the gelatin-PVA scaffold crosslinked and sterilized by gamma radiation followed by freeze-drying was evaluated by cytotoxicity, adhesion and proliferation tests. The cytotoxicity results showed that the biomaterial produced was non-toxic, and adhesion and proliferation assays showed that the material was suitable for tissue engineering. The presence of albumin did not present a significant impact on the cell performance, at the assayed concentration.Resumo IPEN-doc 25541 Development of gelatin based scaffold by gamma radiation for application as platelet rich plasma support for wound treatment2018 - VARCA, JUSTINE P.R.O.; FERRARI, ANDRE; PIGNATA, DANILO R.; GUAZZELLI, STEFANE K.; KLINGBEIL, MARIA F.G.; VARCA, GUSTAVO H.C.; LUGAO, ADEMAR B.; MATHOR, MONICA B.Gelatin is a natural polymer originated from the collagen, and presents poor mechanical properties, however it is a natural and biocompatible polymer, and collagen is the main component of the extracellular matrix1. Poly-vynil (alcohol) is an artificial polymer with interesting mechanical properties and biocompatibility. Such polymers have been largely scientific studied for biomedical application2. Platelet Rich Plasma (PRP) has been widely scientific explored in many medical fields in the last decades, especially in orthopedic area and in athletic treatments3. Based on the PRP desired properties regarded to the tissue regeneration, the present work aimed to develop a scaffold to support PRP release for wound treatment and study the influence of different radiation doses on a scaffold formation to apply as support for PRP release for wound treatment. In specific terms, the impact of radiation will be evaluated through physicochemical and morphologic characteristics. In the study, two polymers of different characteristics were applied, and scaffolds were prepared based on two formulations, one composed by gelatin (7%, w/w) and PVA (5%, w/w), and the second by gelatin (10%, w/w) and PVA (5%, w/w). The formulations were solubilized together in distilled water and heated up to 80 ºC under constant stirring for 1 hour. Posteriorly, the blends were disposed in circular glass molds. The samples were cooled at 4 ºC for at least 24 h and then irradiated at 15, 25 and 50 kGy. After irradiation, the samples were frozen and freeze dried. The scaffolds were characterized in terms of structure and morphology by mechanical assays, differential scanning calorimetry, scanning electron microscopy, optical coherence tomography and infrared spectroscopy. In addition, platelet adhesion and release, and cytotoxic assays were also performed. Samples irradiated at 15 kGy presented pore size diameter of around 1.4 μm and porosity of 54%, while samples irradiated at 25 kGy, presented pore size diameter of around 1.1 μm and porosity of 49%. Optical coherence tomography showed that gelatin control samples presented more superficial degradation as irradiation dose increased, while PVA control sample presented higher integrity, indicating that this polymer is less sensitive to gamma radiation. The system presented suitable mechanical properties and the platelet adhesion and release assays showed that the scaffold presented adequate pore size range to host and release the platelets, and non-cytotoxic to platelets, featuring adequate properties to be applied as dressing for wound treatments.Resumo IPEN-doc 23066 Development of advanced scaffolds and polymeric systems for improved cell and tissue growth2017 - KLINGBEIL, M.; ERNESTO, J.; LOPES, P.; ROCHA, D.; OLIVEIRA, J.; VARCA, G.H.C.; SILVA, C.; STEFFENS, D.; LUGAO, A.B.; MATHOR, M.The recent concern related to skin compromised patients, apart from the nature of the condition itself, such as wounds, chronic ulcers, or burns among others, has triggered and highlighted the importance of the development of artificial skins available in allogeneic donor tissue banks and/or scaffolds, composed by a wide variety of biocompatible, biodegradable and bioactive biomaterials. Within this context, tissue engineering has been in expansion as an attempt to overcome difficulties faced in such situations. The application of scaffolds, produced or not by nanotechnology, in the skin of a patient induces cells to proliferate and get organized on extracellular matrix regenerating tissue. Ionizing radiation is a particularly useful technology capable of promoting sterilization and cross-linking of the scaffold structure thus offering several possibilities for the development of advanced systems suitable for cell growth. Taking into account the variety of clinical applications of tissue engineering, the aim of this study was to investigate by means of histological tests, chemistries and non-destructive tests, the interaction of mesenchymal stem cells grown in vitro in conjunction with different frameworks in order to understand how the mesenchymal stem cells behave in different niches. Among those collagen, PVA, chitosan, PDLLA scaffolds were the systems of choice and -irradiation was applied for sterilization of the systems, as well as cross-linking for the PVA based scaffold. Thus this work allowed the achievement of dermo-epidermic matrices populated by epidermal cells that make up the skin and will contribute to the development of a more robust and useful material to be used in several treatments.