REBECA DA SILVA CANTINHA
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Artigo IPEN-doc 23157 HSP70 expression in Biomphalaria glabrata snails exposed to cadmium2017 - CANTINHA, REBECA da S.; BORRELY, SUELI I.; OGUIURA, NANCY; PEREIRA, CARLOS A. de B.; RIGOLON, MARCELA M.; NAKANO, ELIANAIn this study, the effects of the heavy metal cadmium on the stress protein HSP70 are investigated in freshwater mollusks Biomphalaria glabrata. Adult snails were exposed for 96 h to CdCl2 at concentrations ranging from 0.09 to 0.7 mg L-1 (LC50/96 (h) = 0.34 (0.30-0.37). Time and concentration-dependent increases in the expression of HSP70 were observed at sub-lethal levels in the immunoblotting assay. Further, an increased survival to a lethal heat shock was observed in animals pre-exposed to a nonlethal concentration of cadmium, evidencing the induction of acquired tolerance. The present study demonstrated the inducibility of B. glabrata HSP70 by cadmium, a relevant environmental contaminant, at non-lethal levels, providing evidences that the assessment of HSP70 in B. glabrata can be regarded as a suitable biomarker for ecotoxicological studies.Artigo IPEN-doc 21211 Characterization of 'alfa'-fucosidase and other digestive hydrolases from Biomphalaria glabrata2015 - PERRELLA, NATALIA N.; CANTINHA, REBECA S.; NAKANO, ELIANA; LOPES, ADRIANA R.Artigo IPEN-doc 15152 Effects of high dose rate gamma radiation on survival and reproduction of Biomphalaria glabrata2009 - CANTINHA, REBECA S.; AMARAL, ADEMIR; BORRELY, SUELI I.; NAKANO, ELIANA; SILVA, LUANNA R.S.; MELO, ANA M.M.A.Ionizing radiations are known as mutagenic agents, causing lethality and infertility. This characteristic has motivated its application on animal biological control. In this context, the freshwater snail Biomphalaria glabrata can be considered an excellent experimental model to study effects of ionizing radiations on lethality and reproduction. This work was designed to evaluate effects of 60Co gamma radiation at high dose rate (10.04 kGy/h) on B. glabrata. For this purpose, adult snails were selected and exposed to doses ranging from 20 to 100 Gy, with 10 Gy intervals; one group was kept as control. There was not effect of dose rate in the lethality of gamma radiation; the value of 64,3 Gy of LD50 obtained in our study was similar to that obtained by other authors with low dose rates. Nevertheless, our data suggest that there was a dose rate effect in the reproduction. On all dose levels, radiation improved the production of embryos for all exposed individuals. However, viability indexes were below 6% and, even 65 days after irradiation, fertility was not recovered. These results are not in agreement with other studies using low dose rates. Lethality was obtained in all groups irradiated, and the highest doses presented percentiles of dead animals above 50%. The results demonstrated that doses of 20 and 30 Gy were ideal for population control of B. glabrata. Further studies are needed; nevertheless, this research evidenced great potential of high dose rate gamma radiation on B. glabrata reproductive control.