MARGARETH KAZUYO KOBAYASHI DIAS FRANCO
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Resumo IPEN-doc 28612 POLYana2021 - SEPULVEDA, ANDERSON F.; FRANCO, MARGARETH; YOKAICHIYA, FABIANO; ARAUJO, DANIELE deINTRODUCTION POLYAna is a new executable software developed by SISLIBIO group for rheological analysis of hydrogel and organogel systems and other colloidal materials (nanoparticles and micelles). The software development aims to facilitate the analysis of rheology data associated to both temperature- and frequency-dependent analysis, viscosity and curve flow profiles. OBJECTIVES The software development aims to facilitate the analysis of rheology data associated to both temperature- and frequency-dependent analysis, viscosity and curve flow profiles. MATERIALS AND METHODS From raw data, several models are applied like power-law model for frequency response and curve flow, Boltzmann law to calculate gelation temperature and viscosity response under temperature,Maxwell model to study interchain relationships in addition to other models such as Bingham model, Cross model, and Herschel-Bulkley are also available. POLYana outputs calculates rheological parameters like consistency, adhesion, hysteresis, flow index, G’/G” ratio. DISCUSSION AND RESULTS To validate results obtained from POLYana, same data were analyzed by applying other programs and same mathematical models. In this sense, rheological analysis of Poloxamer 407 in water solution (15 %) were performed: from temperature-dependent G’ and G” analysis were obtained gelation temperature of 45.46 ± 0.02 °C, η_0 = 0.08 ± 0.03 mPa*s, η_max = (32.44 ± 0.17) mPa*s and dη/dT = (1.27 ± 0.02) mPa*s/°C by fitting Boltzmann law (R2 = 0.998), which are similar to results obtained by others softwares and found in literature. From temperature-dependent G’ and G” analysis, it gets adhesion value of (1647.15 ± 18.01) mPa*sn calculated from power-law model (R2 = 0.869), also similar to PRISM results. CONCLUSION Also, other Poloxamer concentrations and hydrogels types have been evaluated, showing close numbers to that previously reported. In order to stablish structural relationships, one of POLYana tools is also to analyze small-angle neutron scattering (SANS) and develop Monte Carlo simulation for SANS and rheological analysis, simultaneously.Resumo IPEN-doc 28606 Evaluation of structural changes of benzocaine-loaded, optimized nanostructured lipid carriers using SANS and Raman imaging approaches2021 - MITSUTAKE, HERY; SOUZA, ALESSANDRO D. de; YOKAICHIYA, FABIANO; FRANCO, MARGARETH K.K.D.; CLEMENS, DANIEL; KENT, BEN; BREITKREITZ, MARCIA C.; RIBEIRO, LIGIA N. de M.; PAULA, ENEIDA de; BORDALLO, HELOISA N.INTRODUCTION Local anesthetics are substances that reversibly block the nerve-impulse conduction, alleviating pain without loss of consciousness. Benzocaine, a poorly soluble local anesthetic, is an ester of para-aminobenzoic acid. Several strategies of formulations can be used to improve bioavailability and decrease adverse effects of benzocaine. In this study nanostructured lipid carriers (NLC) were employed. These lipid-based drug delivery carriers have a lipid core composed of a blend of solid and liquid lipids, and a shelf of non-ionic surfactant. OBJECTIVES The main aim of this work was to optimize benzocaine-loaded NLC and to investigate structural changes in these nanoparticles, under different temperatures. MATERIALS AND METHODS The ratio of excipients (cetyl palmitate, Capmul® PG-8 NF and Pluronic®F68) and benzocaine in the NLC was optimized using a 2 3 factorial design with respect to the following parameters: particle size, polydispersity index (PDI) and zeta potentials. DISCUSSION AND RESULTS The interactions between the factors were found relevant to determine particle size and PDI. Using desirability function, the best formulation conditions were found. Structural changes in optimized NLC were observed with Small-Angle Neutron Scattering (SANS) and Raman imaging, in samples at 27, 37 and 40º C. SANS pointed the formation of lamellar structures inside the NLC, which interlamellar distances increase at higher temperature. Raman imaging showed that the incorporation of P68 and benzocaine in-between the lipids increased at higher temperatures, explaining the changes in Q values (SANS). CONCLUSION This work shows how different scattering techniques can provide complementary information and be used together to characterize and understand the physical, chemical, and structural changes on the organization of pharmaceutical carriers in drug delivery system.Resumo IPEN-doc 28604 Improvement of the methodological strategies to product functionalizes antibodies using Small Angle Neutron Scattering (SANS)2021 - TREMARIN, BEATRIZ; YOKAICHIYA, FABIANO; KELLERMANN, GUINTHER; FRANCO, MARGARETH K.K.D.; STORSBERG, JOACHIMINTRODUCTION Antibodies are used by jawed vertebrates for defense against invading pathogens. Usage of those versatile tools in a plethora of settings in clinics and biomedical sciences hinges on functionalization strategies that retain native antibody reactivity. To this date, antibody functionalization is performed by trial and error. OBJECTIVES We aim to reduce costs by providing general principles to allow the full spectrum of antibody functionalization by correlating functionalized antibody reactivity to cognate antigen by small angle neutron scattering, SANS, measurements and mathematical modeling of antibody and antibody-antigen super-complexes, obtained by titration experiments. MATERIALS AND METHODS For this research we have used for as antibody pure goat anti rabbit immunoglobulin, and for the antigen, pure Horseradish Peroxidase Preliminary results show that the systems (antibody and antibody-antigen complexes) do not change in the range of a temperature related to storage temperature (25º C), body temperature (37º C) and 40º C. DISCUSSION AND RESULTS These results will give us the pair distribution function of these systems and the results will be viewed in light of published precedence to highlight areas where future effort is needed to refine such versatile tools and improve their production. However, between the antibody and the complexes structure, different conformations were observed. The antibody has a globular structure with a radius of gyration around 33 Å, and the complexes display an elongated cylindrical shape with radius of gyration around 63 Å. CONCLUSION This study shows how the scattering techniques (SANS) can provide useful information about the conformation of the antibody and antibody-antigen formation and help to shed light in the understanding the physical, chemical, and structural changes on the organization of these important antibody functionalization for the immunological system.