THIAGO MARTINI PEREIRA

THIAGO MARTINI PEREIRA

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Evaluating biochemical differences in thyroglobulin from normal and goiter tissues by infrared spectral imaging
2020 - PEREIRA, THIAGO M.; DIEM, MAX; BACHMANN, LUCIANO; BIRD, BENJAMIN; MILJKOVIC, MILOS; ZEZELL, DENISE M.
Thyroglobulin is a glycoiodoprotein that is produced by thyroid follicular cells; it is stored in follicles in structures known as colloids. The main function of this protein is to stock the hormones triiodothyronine (T3) and thyroxine (T4) until the body requires them. This study aims to demonstrate that infrared spectral imaging with appropriate multivariate analysis can reveal biochemical changes in this glycoprotein. The results achieved herein point out biochemical differences in the colloid samples obtained from normal and goiter patients including glycosylation and changes in the secondary conformational structure. We have presented the first spectral histopathology-based method to detect biochemical differences in thyroid colloids, such as TG iodination, glycosylation, and changes in the secondary structure in normal and goiter patients. The observed changes in the colloids were mainly due to the alterations in amide I and amide II (secondary conformation of proteins) and there is a correlation with different glycosylation between normal and goiter tissues.
ATR-FTIR spectroscopy for the assessment of biochemical changes in skin due to cutaneous squamous cell carcinoma
2015 - LIMA, CASSIO A.; GOULART, VIVIANE P.; CORREA, LUCIANA; PEREIRA, THIAGO M.; ZEZELL, DENISE M.
Nonmelanoma skin cancers represent 95% of cutaneous neoplasms. Among them, squamous cell carcinoma (SCC) is the more aggressive form and shows a pattern of possible metastatic profile. In this work, we used Fourier transform infrared spectroscopy (FTIR) spectroscopy to assess the biochemical changes in normal skin caused by squamous cell carcinoma induced by multi-stage chemical carcinogenesis in mice. Changes in the absorption intensities and shifts were observed in the vibrational modes associated to proteins, indicating changes in secondary conformation in the neoplastic tissue. Hierarchical cluster analysis was performed to evaluate the potential of the technique to differentiate the spectra of neoplastic and normal skin tissue, so that the accuracy obtained for this classification was 86.4%. In this sense, attenuated total reflection (ATR)-FTIR spectroscopy provides a useful tool to complement histopathological analysis in the clinical routine for the diagnosis of cutaneous squamous cell carcinoma.
Temperature increase at the light guide tip of 15 contemporary LED units and thermal variation at the pulpal floor of cavities: an infrered thermographic analysis
2013 - GOMES, M.; VITO MORAES, A. de; FRANCCI, C.; MORAES, R.; PEREIRA, T.; FROES SALGADO, N.; YAMAZAKI, L.; SILVA, L.; ZEZELL, D.
Temperature changes on dental pulpal chamber during led light curing simulation
2010 - PEREIRA, T.M.; ZEZELL, D.M.; GOMES, M.N.; MORAES, A.G. de V. de; MORAES, R.R.; FROES SALGADO, N.R.G.; FRANCI, C.E.
Non linear index refraction of hap solution using z-scan technique
2010 - SANTOS, MOISES O. dos; ZEZELL, DENISE M.; PEREIRA, THIAGO M.; ALBERO, FELIPE G.; FREITAS, ANDERSON Z. de; SAMAD, RICARDO E.; VIEIRA JUNIOR, NILSON D.; VALERIO, MARIO E.G.; GOMES, ANDERSON S.L.
A thermal investigation of dental bleaching in vitro
2008 - KABBACH, WILLIAM; ZEZELL, DENISE M.; PEREIRA, THIAGO M.; ALBERO, FELIPE G.; CLAVIJO, VICTOR R.G.; ANDRADE, MARCELO F. de
Objective: Our goal was to investigate the surface temperature variations in the cervical region via infrared thermography, as well as the temperature within the pulp chamber via thermocouples, of mandibular incisors when subjected to dental bleaching using two different 35% hydrogen peroxide gels, red (HP) and green (HPM), when activated by halogen light (HL) and LED light. Background Data: Temperatures increases of more than 5.5°C are considered to be potentially threatening to pulp vitality, while those higher than 10°C can result in periodontal injury. Materials and Methods: Tooth samples were randomly divided into four groups (n = 10 each), according to the bleaching agent and catalyst light source used. Results: Mean values and standard deviations of the temperature increases inside the pulp chamber in the HL groups were 4.4° ± 2.1°C with HP, and 4.5° ± 1.2°C with HPM; whereas in the groups using LED light, they were 1.4° ± 0.3°C for HP, and 1.5° ± 0.2°C for HPM. For the root surfaces, the maximum temperature increases in the groups irradiated with HL were 6.5° ± 1.5°C for HP, and 7.5° ± 1.1°C with HPM; whereas in the groups irradiated with LED light, they were 2.8° ± 0.7°C with HP, and 3° ± 0.8°C with HPM. There were no statistically significant differences in pulp and surface temperature increases between the groups using different gels, although the mean temperature increases were significantly higher for the groups irradiated with HL when compared with those irradiated with the LED light (p < 0.05 with Tukey's test). Conclusion: LED light may be safe for periodontal and pulp tissue when using this method, but HL should be used with care.
An in vitro thermal analysis during different light-activated hydrogen peroxide bleaching
2010 - KABBACH, W.; ZEZELL, D.M.; BANDECA, M.C.; PEREIRA, T.M.; ANDRADE, M.F.
Determination and optimization of ATR-FTIR parameters for bone tissue analysis
2010 - BENETTI, CAROLINA; SANTOS, MOISES O. dos; PEREIRA, THIAGO M.; ZEZELL, DENISE M.
Thermal diffusivity measurement of enamel and dentin as a function of temperature obtained by infrared camera
2010 - PEREIRA, THIAGO M.; ZAMATARO, CLAUDIA B.; ZEZELL, DENISE M.
Influence of fixation products used in the histological processing in the FTIR Spectra of lung cells
2012 - PEREIRA, THIAGO M.; DAGLI, MARIA L.Z.; MENNECIER, GREGORY; ZEZELL, DENISE M.