CAROLINA GOUVEA DE SOUZA CONTATORI
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Artigo IPEN-doc 29066 Responses of melanoma cells to photobiomodulation depend on cell pigmentation and light parameters2022 - CONTATORI, CAROLINA G. de S.; SILVA, CAMILA R.; PEREIRA, SAULO de T.; RODRIGUES, MARIA F.S.D.; LUNA, ARTHUR C. de L.; MARQUES, MARCIA M.; RIBEIRO, MARTHA S.Melanoma is a highly aggressive skin cancer that requires new approaches for its management. Low-level laser therapy, currently named photobiomodulation therapy (PBM), has been used to improve different conditions but its effects and safe use on melanoma remain unexplored. Herein, we investigated the PBM impact on melanoma cells differing by pigmentation using near-infrared (NIR) and red lasers in vitro. In vivo, we evaluated the effects of the red laser on melanoma-bearing mice. Amelanotic (SK-MEL-37) and melanotic (B16F10) cells were exposed in vitro to a NIR (780 nm, 40 mW) or a red laser (660 nm, 40 mW) in 3 different light doses: 30, 90, and 150 J/cm2 and responses were assessed regarding mitochondrial activity, invasiveness, migration, and VEGF production. In vivo, melanoma-bearing mice received the red laser delivering 150 J/cm2 directly to the tumor on 3 consecutive days. Mice were monitored for 15 days regarding tumor progression and mouse survival. We noticed that amelanotic cells were unresponsive to NIR light. In contrast, NIR irradiation at 30 J/cm2 promoted an increase in the invasiveness of pigmented cells, even though all light doses have inhibited cell migration. Regarding the red laser on pigmented cells, the highest light dose (150 J/cm2) decreased the VEGF production and migration. In vivo, melanoma-bearing mice treated with red laser showed smaller tumor volume and longer survival than controls. We conclude that PBM appears to be safe for amelanotic non-pigmented melanoma but triggers different responses in melanotic pigmented cells depending on light parameters. Additionally, a high dose of red laser impairs the invasive behavior of melanoma cells, probably due to the decrease in VEGF synthesis, which may have contributed to tumor arrest and increased mouse survival. These findings suggest that red laser therapy could be a new ally in the supportive care of melanoma patients.Resumo IPEN-doc 28901 Efeitos da fotobiomodulação pré-exposição à radiação ionizante em células de câncer de mama2021 - SILVA, CAMILA R.; PEREIRA, SAULO T.; CONTATORI, CAROLINA G.S.; PINTO, MAYARA S.; SALVEGO, CAMILA A.; RIBEIRO, MARTHA S.Resumo IPEN-doc 25971 Photobiomodulation can delay tumor progression in breast cancer bearing-mice2018 - SILVA, CAMILA R.; CONTATORI, CAROLINA G. de S.; PEREIRA, SAULO de T.; RIBEIRO, MARTHA S.Cancer is a worldwide health problem and new therapeutic strategies are necessary. Photobiomodulation is a noninvasive and cost-effective therapy, but its use in cancer cells is still controversial. In this study, we explore the effects of PBM on breast tumor bearing-mice.Resumo IPEN-doc 25504 Low level light therapy on breast tumor. In vitro and in vivo studies2018 - PEREIRA, SAULO; CONTATORI, CAROLINA; RIBEIRO, MARTHA S.Low Level Light Therapy (LLLT) has been gaining prominence in health areas due to search for less invasive and cost-effective treatments. However, its use is not indicated to treat patients with cancer due to a few studies in vivo. The aim of this work was to evaluate the effects of LLLT on breast tumor 4T1 cells in vitro and in a murine model. For the in vitro assay, 4T1 cells were submitted to a single session of LLLT with red light (660 ± 20 nm) delivering energies of 1.2 J and 6 J. The cell viability was measured by MTT assay. For in vivo study, nine BALB/c female mice (6 weeks old) received 4T1 cells transfected with luciferase (4T1-Luc) into the mammary fat pad. After two weeks, the animals were divided into control (n=3) and test groups were submitted to LLLT with same parameters described above. The tumor progression was monitored by a caliper and bioluminescence. The anti-fatigue effect of LLLT was assessed by a forced swimming test. Our results showed that LLLT was not able to increase cell viability regardless the energy used. In vivo, the lower energy promoted an increase of the tumor volume compared to control group. On the other hand, the higher energy was able to arrest tumor progression as well as increase the swimming time. Taken together, our results suggest that LLLT triggers opposite effects on breast tumor depending on the type of assay.