SOLANGE KAZUMI SAKATA

(Fonte: Lattes)
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Possui graduaĆ§Ć£o em QuĆ­mica bacharelado e licenciatura pela Universidade de SĆ£o Paulo. Doutorado na Ć”rea de QuĆ­mica OrgĆ¢nica, com ĆŖnfase em Eletrossintese OrgĆ¢nica pelo Instituto de QuĆ­mica da Universidade de SĆ£o Paulo. PĆ³s - doutorados em Biotecnologia no Scripps Institution of Oceanography na University of California - San Diego -USA) e no Instituto de QuĆ­mica da Universidade de SĆ£o Paulo. Foi pesquisadora visitante no Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB-Stuttgart - Alemanha no estudo do metagenoma na produĆ§Ć£o de enzimas para fins catalĆ­ticos e no Centro TecnolĆ³gico da Marinha de SĆ£o Paulo (CTM-SP) no desenvolvimento e caracterizaĆ§Ć£o de polĆ­meros. Atualmente Ć© pesquisadora do Instituto de Pesquisas EnergĆ©ticas e Nucleares (IPEN- SP / CNEN) no Centro de Tecnologia das RadiaƧƵes e estuda o efeitos das radiaƧƵes em nano materiais de carbono. (Texto extraĆ­do do CurrĆ­culo Lattes em 27 dez. 2021).

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    Artigo IPEN-doc 29599
    Sandwich-like electrochemical aptasensing of heat shock protein 70 kDa (HSP70)
    2023 - NEGAHDARY, MASOUD; HIRATA, MARIO H.; SAKATA, SOLANGE K.; CICONELLI, ROZANA M.; BASTOS, GISELE M.; BORGES, JESSICA B.; THUROW, HELENA S.; SILVEIRA JUNIOR, ALCEU T.; SAMPAIO, MARCELO F.; GUIMARAES, LARISSA B.; MAEDA, BRUNO S.; ANGNES, LUCIO
    In this research, by using aptamer-conjugated gold nanoparticles (aptamer-AuNPs) and a modified glassy carbon electrode (GCE) with reduced graphene oxide (rGO) and Acropora-like gold (ALG) nanostructure, a sandwich-like system provided for sensitive detection of heat shock protein 70 kDa (HSP70), which applied as a functional biomarker in diagnosis/prognosis of COVID-19. Initially, the surface of the GCE was improved with rGO and ALG nanostructures, respectively. Then, an aptamer sequence as the first part of the bioreceptor was covalently bound on the surface of the GCE/rGO/ALG nanostructures. After adding the analyte, the second part of the bioreceptor (aptamer-AuNPs) was immobilized on the electrode surface to improve the diagnostic performance. The designed aptasensor detected HSP70 in a wide linear range, from 5 pg mLāˆ’1 to 75 ng mLāˆ’1, with a limit of detection (LOD) of āˆ¼2 pg mLāˆ’1. The aptasensor was stable for 3 weeks and applicable in detecting 40 real plasma samples of COVID-19 patients. The diagnostic sensitivity and specificity were 90% and 85%, respectively, compared with the reverse transcription-polymerase chain reaction (RT-PCR) method.