Correlation between autofluorescence intensity and tumor area in mice bearing renal cell carcinoma
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Journal of Fluorescence
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Protoporphyrin IX (PpIX) is a porphyrin derivative that is accumulated in cancerous tissue in consequence of the tumor-specific metabolic alterations. The aim
of this study was to evaluate the accumulation of PpIX in
mice bearing renal cell carcinoma by spectroscopy analysis.
A total of 24 male Balb/c mice, 6 weeks old, were divided
into six groups: Normal (without inoculation of tumor cells)
and 4, 8, 13, 16, and 20 days after inoculation of tumor
cells. The orthotopic tumor model of renal cancer was used.
Murine renal cell carcinoma (Renca cells) were inoculated
into the subcapsular space of the kidney. Normal and
tumor-bearing kidneys in different progression stages were
removed and analyzed by ex-vivo spectroscopy and by
microscopy, for tumor histometric analysis. Emission
spectra were obtained by exciting the samples at 405 nm.
Significant differences between normal and tumor-bearing
kidneys in autofluorescence shape occurred in the 600–700
nm spectral region. A good correlation was found between
emission band intensity at 635 nm and the tumor area.
Como referenciar
BELLINI, MARIA H.; COUTINHO, ENIA L.; COURROL, LILIA C.; SILVA, FLAVIA R. de O.; VIEIRA JUNIOR, NILSON D.; SCHOR, NESTOR. Correlation between autofluorescence intensity and tumor area in mice bearing renal cell carcinoma. Journal of Fluorescence, v. 18, n. 6, p. 1163-1168, 2008. DOI: 10.1007/s10895-008-0368-4. Disponível em: http://repositorio.ipen.br/handle/123456789/4990. Acesso em: 27 Mar 2026.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.