Radiolabeling of anti-cd20 with Re-188: liquid kit

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dc.contributor.authorDIAS, CARLA R.pt_BR
dc.contributor.authorOSSO JUNIOR, JOAO A.pt_BR
dc.coverageInternacionalpt_BR
dc.creator.eventoINTERNATIONAL NUCLEAR ATLANTIC CONFERENCE; MEETING ON NUCLEAR APPLICATIONS, 8th/ MEETING ON REACTOR PHYSICS AND THERMAL HYDRAULICS, 15thpt_BR
dc.date.accessioned2014-11-17T18:41:42Zpt_BR
dc.date.accessioned2014-11-18T18:16:08Zpt_BR
dc.date.accessioned2015-04-02T05:41:15Z
dc.date.available2014-11-17T18:41:42Zpt_BR
dc.date.available2014-11-18T18:16:08Zpt_BR
dc.date.available2015-04-02T05:41:15Z
dc.date.eventoSept. 30 - Oct. 5, 2007pt_BR
dc.description.abstractRadioimmunotherapy uses the targeting features of monoclonal antibody to deliver radiation from an attached radionuclide. The radionuclide 188Re is currently produced from the father nuclide 188W through a transportable generator system. Because of its easy availability and suitable nuclear properties (EβMAX =2.1 MeV, t1/2=16.9 h, Eγ=155 keV), this radionuclide is considered an attractive candidate for application as therapeutic agents and could be conveniently utilized for imaging and dosimetric purposes. The objective of this work is the optimization of radiolabeling of anti-CD20 with 188Re using a liquid formulation. Anti-CD20 was reduced by incubation with 2-ME and purified over a PD-10 column. The number of resulting free SH was assayed with Ellman’s reagent. Optimization of radiolabeling was achieved by varying parameters: antibody mass, reducing agent, reaction time and 188Re volume in the liquid kit. Radiochemical purity of 188Re-anti-CD20 was evaluated. An average of 12 SH groups per mol in the reductions was found. The best labeling efficiency (> 93%) was achieved in the following conditions: 1 mg anti-CD20; 82.8 mg sodium tartrate; 1 mg SnCl2; 0.25 mg gentisic acid, 1 mL 188Re and reaction time of 1 hour at room temperature.
dc.event.siglaINAC; ENAN; ENFIRpt_BR
dc.identifier.citationDIAS, CARLA R.; OSSO JUNIOR, JOAO A. Radiolabeling of anti-cd20 with Re-188: liquid kit. In: INTERNATIONAL NUCLEAR ATLANTIC CONFERENCE; MEETING ON NUCLEAR APPLICATIONS, 8th/ MEETING ON REACTOR PHYSICS AND THERMAL HYDRAULICS, 15th, Sept. 30 - Oct. 5, 2007, Santos, SP. <b>Proceedings...</b> Disponível em: http://repositorio.ipen.br/handle/123456789/18110.
dc.identifier.orcidhttps://orcid.org/0000-0002-6672-1631
dc.identifier.urihttp://repositorio.ipen.br/handle/123456789/18110pt_BR
dc.local.eventoSantos, SPpt_BR
dc.publisherSao Paulo: ABEN, 2007pt_BR
dc.rightsopenAccesspt_BR
dc.subjectactivity levelspt_BR
dc.subjectelutriationpt_BR
dc.subjectexperimental datapt_BR
dc.subjectimpuritiespt_BR
dc.subjection exchangept_BR
dc.subjectisotope productionpt_BR
dc.subjectlutetium 176pt_BR
dc.subjectlutetium 177pt_BR
dc.subjectnuclear reactionspt_BR
dc.subjectytterbium 175pt_BR
dc.subjectytterbium 176 targetpt_BR
dc.subjectytterbium 177pt_BR
dc.titleRadiolabeling of anti-cd20 with Re-188: liquid kitpt_BR
dc.typeTexto completo de eventopt_BR
dspace.entity.typePublication
ipen.autorJOAO ALBERTO OSSO JUNIOR
ipen.autorCARLA ROBERTA DE BARROS RODRIGUES DIAS
ipen.codigoautor140
ipen.codigoautor2715
ipen.contributor.ipenauthorJOAO ALBERTO OSSO JUNIOR
ipen.contributor.ipenauthorCARLA ROBERTA DE BARROS RODRIGUES DIAS
ipen.date.recebimento07-12pt_BR
ipen.event.datapadronizada2007pt_BR
ipen.identifier.ipendoc12107pt_BR
ipen.notas.internasProceedingspt_BR
ipen.type.genreArtigo
relation.isAuthorOfPublicationb0d5c854-8fcb-4e5f-b1c9-73e82c9ca844
relation.isAuthorOfPublicationc8caef90-0b8a-4c08-bcec-cd3f72419253
relation.isAuthorOfPublication.latestForDiscoveryc8caef90-0b8a-4c08-bcec-cd3f72419253
sigepi.autor.atividadeDIAS, CARLA R.:2715:12:Spt_BR
sigepi.autor.atividadeOSSO JUNIOR, JOAO A.:140:12:Npt_BR
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