Radiolabeling of anti-cd20 with Re-188: liquid kit
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2007
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INTERNATIONAL NUCLEAR ATLANTIC CONFERENCE; MEETING ON NUCLEAR APPLICATIONS, 8th/ MEETING ON REACTOR PHYSICS AND THERMAL HYDRAULICS, 15th
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Resumo
Radioimmunotherapy uses the targeting features of monoclonal antibody to deliver radiation from an attached
radionuclide. The radionuclide 188Re is currently produced from the father nuclide 188W through a transportable
generator system. Because of its easy availability and suitable nuclear properties (EβMAX =2.1 MeV, t1/2=16.9 h,
Eγ=155 keV), this radionuclide is considered an attractive candidate for application as therapeutic agents and
could be conveniently utilized for imaging and dosimetric purposes. The objective of this work is the
optimization of radiolabeling of anti-CD20 with 188Re using a liquid formulation. Anti-CD20 was reduced by
incubation with 2-ME and purified over a PD-10 column. The number of resulting free SH was assayed with
Ellman’s reagent. Optimization of radiolabeling was achieved by varying parameters: antibody mass, reducing
agent, reaction time and 188Re volume in the liquid kit. Radiochemical purity of 188Re-anti-CD20 was evaluated.
An average of 12 SH groups per mol in the reductions was found. The best labeling efficiency (> 93%) was
achieved in the following conditions: 1 mg anti-CD20; 82.8 mg sodium tartrate; 1 mg SnCl2; 0.25 mg gentisic
acid, 1 mL 188Re and reaction time of 1 hour at room temperature.
Como referenciar
DIAS, CARLA R.; OSSO JUNIOR, JOAO A. Radiolabeling of anti-cd20 with Re-188: liquid kit. In: INTERNATIONAL NUCLEAR ATLANTIC CONFERENCE; MEETING ON NUCLEAR APPLICATIONS, 8th/ MEETING ON REACTOR PHYSICS AND THERMAL HYDRAULICS, 15th, Sept. 30 - Oct. 5, 2007, Santos, SP. Proceedings... Disponível em: http://repositorio.ipen.br/handle/123456789/18110. Acesso em: 23 Feb 2025.
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