Synthesis of radioactive gold 198 nanoparticle encapsulated by albumin as cancer theranostics agent

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dc.contributor.authorLUGAO, A.B.
dc.contributor.authorGERALDES, A.N.
dc.contributor.authorLEAL, J.
dc.contributor.authorVARCA, G.H.C.
dc.contributor.authorBATISTA, J.G.S.
dc.contributor.authorGRASSELLI, M.
dc.contributor.authorKATTI, K.
dc.contributor.authorBARROS, J.
dc.coverageInternacionalpt_BR
dc.creator.eventoCONGRESSO LATINO-AMERICANO DE ORGAOS ARTIFICIAIS E BIOMATERIAIS, 9; CONGRESSO DA SOCIEDADE LATINO AMERICANA DE BIOMATERIAIS, ORGAOS ARTIFICIAIS E ENGENHARIA DE TECIDOS, 13.pt_BR
dc.date.accessioned2017-05-19T13:36:00Z
dc.date.available2017-05-19T13:36:00Z
dc.date.evento24-27 de agosto, 2016pt_BR
dc.description.abstractAlbumin is a natural drug nanocarrier as it has equivalent diameter of about 5 nm and has natural affinity for hydrophobic and hydrophilic drugs. They can be bound in a reversible way and the delivery controlled by endogenous mechanism. Albumin can be crosslinked by radiation alternatively to conventional systems, in such way that dialdehydes or toxic chemicals are avoided. On the theranostic side, radioactive ions are commonly employed for diagnostic and therapeutic applications. As an example, radioactive gold nanoparticles are currently employed in radiotherapy whether to increase local dose deposition in tissue during radiotherapy or as a local emitter of gamma and beta rays. The radioactive properties of gold include: 198Au (?max=0.96 MeV; t1/2 = 2.7 days) and 199Au (?max =0.46 MeV; t1/2 = 3.14 days), making it a strong candidate for theranostics . Conjugation of such materials with sugars, peptides, antibodies, proteins among others is routinely used nowadays for targeting. While radioactive nanoparticles can offer a much higher dose payload than ions for therapy and diagnostic, in addition to the the huge surface to bind targeting species presented by the nanoparticles, functionalization with proteins may potentially increases the particle uptake by tumors or tissues. The main purpose of this work was the development of one pot in situ synthesis of radioactive gold 198 nanoparticle encapsulated by albumin for application in cancer Theranostics. While crosslinked albumin may provide a nontoxic coating on AuNPs with a controllable hydrodynamic diameter, conventional AuNP can be activated by nuclear reactor to produce 198AuNP. The gamma or beta radiation originated from the gold nanoparticle was used to crosslink the Albumin layer. The use of a radioactive particle able to emit radiation for crosslinking of the Albumin layer and simultaneous theranostic application was tried for the first time. The elegant procedure and ease of produc¬tion combined with the properties of 198Au and the safety of HSA-198AuNP make this new particle an exciting advancement in cancer therapy and diagnosis. For such purpose, radioactive tetrachloroauric acid H198AuCl4 was produced from gold foils of high purity by neutron irradiation in IPEN research nuclear reactor. 198AuNP/BSA were synthesized by stirring aqueous solutions of BSA and radioactive tetrachloroauric acid H198AuCl4. The reaction mixtures were stirred continuously at 25 °C. The color of the mixture become purple-red from pale yellow within 15 minutes indicating the formation of gold nanoparticles. The reaction mixture was stirred for an additional 20 minutes. The 198AuNP/BSA formed were characterized by UV-Vis spectrophotometry, DLS and TEM analysis. The spectra were recorded at first day of preparation and after 1 month. Radioactive gold nanoparticle encapsulated by crosslinked Albumin was prepared in reproducible way. The gold nanoparticle core size measured by TEM was about 20 nm and about 60 to 70 nm with the albumin layer as measured by DLS. Bityrosine formation was measured by fluorescence and it was an evidence of intramolecular and intermolecular crosslinking. In conclusion the technique was suitable for the in situ production of the crosslinked albumin functionalized radioactive gold nanoparticles. Acknowledgements The authors would like to thank Conselho Nacional de Desenvolvimento Cientifico e Tecnológico (CNPq) project number 402887/2013-1 and 401438/2014-7 and International Atomic Energy Agency (IAEA) (CRP code F22064 ) for financial support.pt_BR
dc.event.siglaSLABOpt_BR
dc.identifier.citationLUGAO, A.B.; GERALDES, A.N.; LEAL, J.; VARCA, G.H.C.; BATISTA, J.G.S.; GRASSELLI, M.; KATTI, K.; BARROS, J. Synthesis of radioactive gold 198 nanoparticle encapsulated by albumin as cancer theranostics agent. In: CONGRESSO LATINO-AMERICANO DE ORGAOS ARTIFICIAIS E BIOMATERIAIS, 9; CONGRESSO DA SOCIEDADE LATINO AMERICANA DE BIOMATERIAIS, ORGAOS ARTIFICIAIS E ENGENHARIA DE TECIDOS, 13., 24-27 de agosto, 2016, Foz do Iguaçu, PR. <b>Abstract...</b> Disponível em: http://repositorio.ipen.br/handle/123456789/27469.
dc.identifier.orcidhttps://orcid.org/0000-0002-1737-3191
dc.identifier.urihttp://repositorio.ipen.br/handle/123456789/27469
dc.local.eventoFoz do Iguaçu, PRpt_BR
dc.rightsopenAccesspt_BR
dc.titleSynthesis of radioactive gold 198 nanoparticle encapsulated by albumin as cancer theranostics agentpt_BR
dc.typeResumo de eventos científicospt_BR
dspace.entity.typePublication
ipen.autorJESSICA LEAL
ipen.autorJORGE GABRIEL DOS SANTOS BATISTA
ipen.autorJANAINA ALINE GALVÃO BARROS
ipen.autorGUSTAVO HENRIQUE COSTAVARCA
ipen.autorADRIANA NAPOLEAO GERALDES
ipen.autorADEMAR BENEVOLO LUGAO
ipen.codigoautor11679
ipen.codigoautor10274
ipen.codigoautor14272
ipen.codigoautor8661
ipen.codigoautor3640
ipen.codigoautor339
ipen.contributor.ipenauthorJESSICA LEAL
ipen.contributor.ipenauthorJORGE GABRIEL DOS SANTOS BATISTA
ipen.contributor.ipenauthorJANAINA ALINE GALVÃO BARROS
ipen.contributor.ipenauthorGUSTAVO HENRIQUE COSTAVARCA
ipen.contributor.ipenauthorADRIANA NAPOLEAO GERALDES
ipen.contributor.ipenauthorADEMAR BENEVOLO LUGAO
ipen.date.recebimento17-05pt_BR
ipen.event.datapadronizada2016pt_BR
ipen.identifier.ipendoc23767pt_BR
ipen.notas.internasAbstractpt_BR
ipen.type.genreResumo
relation.isAuthorOfPublication4718f72f-9574-4e63-8272-371bfddc8de6
relation.isAuthorOfPublicationd71d578f-dbbd-4ff9-adf8-929b13d23037
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relation.isAuthorOfPublication99ac24c5-2ae1-465a-a6f2-40b4d9af6af7
relation.isAuthorOfPublication.latestForDiscovery99ac24c5-2ae1-465a-a6f2-40b4d9af6af7
sigepi.autor.atividadeLUGAO, A.B.:339:740:Spt_BR
sigepi.autor.atividadeGERALDES, A.N.:3640:-1:Npt_BR
sigepi.autor.atividadeLEAL, J.:11679:-1:Npt_BR
sigepi.autor.atividadeVARCA, G.H.C.:8661:750:Npt_BR
sigepi.autor.atividadeBATISTA, J.G.S.:10274:750:Npt_BR
sigepi.autor.atividadeBARROS, J.:14272:-1:Npt_BR
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