Development of radioimmunoconjugate for diagnosis and management of head-and-neck subclinical cancer and colorectal carcinoma

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dc.contributor.authorBENEDETTO, RAQUEL
dc.contributor.authorMASSICANO, ADRIANA V.F.
dc.contributor.authorSILVA, JEFFERSON J.
dc.contributor.authorBOAS, CRISTIAN A.W.V.
dc.contributor.authorMENGATTI, JAIR
dc.contributor.authorARAUJO, ELAINE B. de
dc.coverageInternacionalpt_BR
dc.date.accessioned2018-07-11T19:53:46Z
dc.date.available2018-07-11T19:53:46Z
dc.date.issued2018pt_BR
dc.description.abstractScientific innovations in diagnostic methods are important drivers of cancer control and prevention. Noninvasive imaging of the epidermal growth factor receptor (EGFR) in head-and-neck squamous, cell carcinoma and colorectal cancer could be valuable to select patients for EGFR-targeted therapy, as well as to monitor the efficacy and occurrence of resistance to immunotherapy. In order to develop the first Brazilian radioimmunoconjugate for diagnosis, Cetuximab has been conjugated to p-SCN-Bn-DTPA chelator and radiolabeled with Indium-111. The conjugation methodology was optimized using different mAb:DTPA molar ratios, time was then reduced for immunoconjugate preparation, besides the protein recovery’ percentage increased after purification (m = 83.8 ± 0.91 %). The stability of Cetuximab-DTPA at – 20 oC was evaluated for six months, and its integrity was greater than 90% (m =93.9 ± 1.5%, N = 24). The radioimmunoconjugate with specific activity of 185 MBq/mg showed radiochemical purity above 95% (m=96.8 ± 1.31 %, N = 15). We conclude that the radioimmunoconjugate 111In-DTPA-cetuximab is stable and may be applied to the diagnosis of EGFR-positive tumors.pt_BR
dc.format.extent1-10pt_BR
dc.identifier.citationBENEDETTO, RAQUEL; MASSICANO, ADRIANA V.F.; SILVA, JEFFERSON J.; BOAS, CRISTIAN A.W.V.; MENGATTI, JAIR; ARAUJO, ELAINE B. de. Development of radioimmunoconjugate for diagnosis and management of head-and-neck subclinical cancer and colorectal carcinoma. <b>Brazilian Journal of Pharmaceutical Sciences</b>, v. 53, n. 4, p. 1-10, 2018. DOI: <a href="https://dx.doi.org/10.1590/s2175-97902017000417039">10.1590/s2175-97902017000417039</a>. Disponível em: http://repositorio.ipen.br/handle/123456789/28929.
dc.identifier.doi10.1590/s2175-97902017000417039pt_BR
dc.identifier.fasciculo4pt_BR
dc.identifier.issn1984-8250pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0003-0456-5589
dc.identifier.percentilfi4.31en
dc.identifier.percentilfiCiteScore30.00
dc.identifier.urihttp://repositorio.ipen.br/handle/123456789/28929
dc.identifier.vol53pt_BR
dc.relation.ispartofBrazilian Journal of Pharmaceutical Sciencespt_BR
dc.rightsopenAccesspt_BR
dc.subjectradioimmunodetection
dc.subjectdiagnostic techniques
dc.subjectcarcinomas
dc.subjectlarge intestine
dc.subjecthead
dc.subjectneck
dc.subjectneoplasms
dc.titleDevelopment of radioimmunoconjugate for diagnosis and management of head-and-neck subclinical cancer and colorectal carcinomapt_BR
dc.typeArtigo de periódicopt_BR
dspace.entity.typePublication
ipen.autorRAQUEL BENEDETTO
ipen.autorCRISTIAN ANTONIO WIECZOREK VILLAS BOAS
ipen.autorELAINE BORTOLETI DE ARAUJO
ipen.autorJAIR MENGATTI
ipen.autorJEFFERSON DE JESUS SILVA
ipen.autorADRIANA VIDAL FERNANDES MASSICANO
ipen.codigoautor12108
ipen.codigoautor14503
ipen.codigoautor664
ipen.codigoautor1498
ipen.codigoautor14158
ipen.codigoautor10449
ipen.contributor.ipenauthorRAQUEL BENEDETTO
ipen.contributor.ipenauthorCRISTIAN ANTONIO WIECZOREK VILLAS BOAS
ipen.contributor.ipenauthorELAINE BORTOLETI DE ARAUJO
ipen.contributor.ipenauthorJAIR MENGATTI
ipen.contributor.ipenauthorJEFFERSON DE JESUS SILVA
ipen.contributor.ipenauthorADRIANA VIDAL FERNANDES MASSICANO
ipen.date.recebimento18-07pt_BR
ipen.identifier.fi0.512pt_BR
ipen.identifier.fiCiteScore0.8
ipen.identifier.ipendoc24713pt_BR
ipen.identifier.iwosWoSpt_BR
ipen.identifier.ods3
ipen.range.fi0.001 - 1.499
ipen.range.percentilfi0.00 - 24.99
ipen.type.genreArtigo
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sigepi.autor.atividadeBENEDETTO, RAQUEL:12108:110:Spt_BR
sigepi.autor.atividadeMASSICANO, ADRIANA V.F.:10449:-1:Npt_BR
sigepi.autor.atividadeMENGATTI, JAIR:1498:110:Npt_BR
sigepi.autor.atividadeARAUJO, ELAINE B. DE:664:110:Npt_BR
sigepi.autor.atividadeSILVA, JEFFERSON J.:14158:110:N
sigepi.autor.atividadeBOAS, CRISTIAN A.W.V.:14503:110:N
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