Cytotoxic and genotoxic effects of I-131 and Co-60 in follicular thyroid cancer cell (WRO) with and without recombinant human thyroid-stimulating hormone treatment

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dc.contributor.authorVALGODE, FLAVIA G.S.
dc.contributor.authorSILVA, MARCIA A. da
dc.contributor.authorVIEIRA, DANIEL P.
dc.contributor.authorRIBELA, MARIA T.C.P.
dc.contributor.authorBARTOLINI, PAOLO
dc.contributor.authorOKAZAKI, KAYO
dc.coverageInternacionalpt_BR
dc.date.accessioned2017-10-05T17:13:34Z
dc.date.available2017-10-05T17:13:34Z
dc.date.issued2017pt_BR
dc.description.abstractNormally, differentiated thyroid cancer (DTC) tends to be biologically indolent, highly curable and has an excellent prognosis. However, the treatment may fail when the cancer has lost radioiodine avidity. The present study was carried out in order to evaluate the cytotoxic and genotoxic effects of I-131 and Co-60 and radioiodine uptake in WRO cells, derived from DTC, harboring the BRAF(V600E) mutation. WRO cells showed a relatively slow cell cycle of 96.3 h with an unstable karyotype containing various double minutes. The genotoxicity assay (micronucleus test) showed a relative high radioresistance to I-131 (0.07-3.70 MBq/mL), independent of treatment with recombinant human thyroid-stimulating hormone (rhTSH). For the cytotoxicity assay, WRO cells were also relatively resistant to Co-60 (range: 0.2-8.3 Gy), but with a gradual decrease of viability as a function of time for higher doses (20 and 40 Gy, starting from the fifth to sixth day). For internal irradiation with I-131, WRO cells showed a decline in viability at radioactive concentration higher than 1.85 MBq/mL; this was even more effective at 3.70 MBq/mL, but only when preceded by rhTSH, in coincidence with the highest level of I-131 uptake. These data show promising results, since the loss of the ability of thyroid cells to concentrate radioiodine is considered to be one of the main factors responsible for the failure of I-131 therapy in patients with DTC. The use of tumor-derived cell lines as a model for in vivo tumor requires, however, further investigations and deep evaluation of the corresponding in vivo effects.pt_BR
dc.format.extent451-461pt_BR
dc.identifier.citationVALGODE, FLAVIA G.S.; SILVA, MARCIA A. da; VIEIRA, DANIEL P.; RIBELA, MARIA T.C.P.; BARTOLINI, PAOLO; OKAZAKI, KAYO. Cytotoxic and genotoxic effects of I-131 and Co-60 in follicular thyroid cancer cell (WRO) with and without recombinant human thyroid-stimulating hormone treatment. <b>Environmental and Molecular Mutagenesis</b>, v. 58, n. 6, p. 451-461, 2017. DOI: <a href="https://dx.doi.org/10.1002/em.22099">10.1002/em.22099</a>. Disponível em: http://repositorio.ipen.br/handle/123456789/27843.
dc.identifier.doi10.1002/em.22099pt_BR
dc.identifier.fasciculo6pt_BR
dc.identifier.issn0893-6692pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-0007-534X
dc.identifier.percentilfi69.20en
dc.identifier.urihttp://repositorio.ipen.br/handle/123456789/27843
dc.identifier.vol58pt_BR
dc.relation.ispartofEnvironmental and Molecular Mutagenesispt_BR
dc.rightsclosedAccesspt_BR
dc.subjecttoxicity
dc.subjectthyroid
dc.subjecttherapy
dc.subjectcarcinomas
dc.subjectthyroid hormones
dc.subjectiodine 131
dc.subjectcobalt 60
dc.titleCytotoxic and genotoxic effects of I-131 and Co-60 in follicular thyroid cancer cell (WRO) with and without recombinant human thyroid-stimulating hormone treatmentpt_BR
dc.typeArtigo de periódicopt_BR
dspace.entity.typePublication
ipen.autorPAOLO BARTOLINI
ipen.autorKAYO OKAZAKI
ipen.autorMARIA TERESA DE CARVALHO PINTO RIBELA
ipen.autorDANIEL PEREZ VIEIRA
ipen.autorMARCIA AUGUSTA DA SILVA
ipen.autorFLAVIA GOMES SILVA VALGODE
ipen.codigoautor1503
ipen.codigoautor1220
ipen.codigoautor1197
ipen.codigoautor3158
ipen.codigoautor2589
ipen.codigoautor3927
ipen.contributor.ipenauthorPAOLO BARTOLINI
ipen.contributor.ipenauthorKAYO OKAZAKI
ipen.contributor.ipenauthorMARIA TERESA DE CARVALHO PINTO RIBELA
ipen.contributor.ipenauthorDANIEL PEREZ VIEIRA
ipen.contributor.ipenauthorMARCIA AUGUSTA DA SILVA
ipen.contributor.ipenauthorFLAVIA GOMES SILVA VALGODE
ipen.date.recebimento17-10pt_BR
ipen.identifier.fi3.254pt_BR
ipen.identifier.ipendoc23214pt_BR
ipen.identifier.iwosWoSpt_BR
ipen.identifier.ods3
ipen.range.fi3.000 - 4.499
ipen.range.percentilfi50.00 - 74.99
ipen.type.genreArtigo
relation.isAuthorOfPublication7d228133-8477-43fb-941d-2cfb6a48c46c
relation.isAuthorOfPublication131eab71-499a-48c8-8f16-46ffafd0608f
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sigepi.autor.atividadeVALGODE, FLAVIA G.S.:3927:810:Spt_BR
sigepi.autor.atividadeSILVA, MARCIA A. DA:2589:-1:Npt_BR
sigepi.autor.atividadeVIEIRA, DANIEL P.:3158:810:Npt_BR
sigepi.autor.atividadeRIBELA, MARIA T.C.P.:1197:810:Npt_BR
sigepi.autor.atividadeBARTOLINI, PAOLO:1503:810:Npt_BR
sigepi.autor.atividadeOKAZAKI, KAYO:1220:810:Npt_BR
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