Gold nanoparticles synthetized with aminolevulinic acid for theranostic

Abstract

In this study we propose to synthesize gold nanoparticles with 5-ALA, to study cytotoxicity in cell cultures, to perform photodynamic (PDT) and sonodynamic (SDT) therapies and to compare the efficience of 5-ALA delivery. For 5-ALA:AuNPs synthesis, is used a 300 Watt Xenon lamp for photo reduction. Cytotoxicity tests were performed on 96-well plates with THP-1 human cells, which were differentiated into macrophages. For the PDT, an amber LED at 590 nm (~ 100mW) was used for 2 minutes and SDT was used with pulses of 1 MHz and 1W / cm2, for 2 minutes. Cellular viability assays (MTS) were performed to evaluate therapies. The results have shown improved photosensitizer delivery in cells incubated with 5-ALA:AuNPs, leading to cell death and indicating potential use as a photo and sonosensitizer agent.